anti-Human HER2 antibody for Immunofluorescence (fixed cells)

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • ERBB2
  • Erbb2
Epitope
AA 1-652, Extracellular Domain
101
95
72
68
61
58
55
55
48
35
26
22
21
21
21
20
20
20
20
20
20
20
20
20
19
19
19
17
17
15
15
13
13
12
11
8
8
8
8
8
7
7
6
6
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1386
502
325
36
34
15
13
8
6
5
4
3
3
3
2
1
1
1
1
1
Host
Rabbit
1006
523
38
16
13
5
1
Clonality
Monoclonal
Conjugate
This HER2 antibody is conjugated to PE
76
66
59
54
47
41
21
21
14
14
13
12
12
9
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
721
602
333
321
309
308
269
134
112
102
91
22
21
19
7
6
6
5
5
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN1995358
$ 198.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1995350 FACS IF IF/ICC FITC Mouse IgG1 AA 1-652 Log in to see 07
1 ABIN1995351 ELISA FACS IF IF/ICC IHC (p) Rabbit IgG AA 1-652, Extracellular Domain Log in to see 002
1 ABIN1995353 CC ELISA FACS IF IF/ICC Rabbit IgG AA 1-652, Extracellular Domain Log in to see 511
1 ABIN2681941 FACS IF IF/ICC Mouse IgG1 AA 1-652 Log in to see 03
1 ABIN2988664 FACS IF/ICC IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN3070843 IF/ICC IHC IP WB Rabbit C-Term, pTyr1248 Log in to see Polyclonal
1 ABIN3003360 IF/ICC Rabbit IgG Log in to see Polyclonal

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 1-652, Extracellular Domain
(101), (95), (72), (68), (61), (58), (55), (55), (48), (35), (26), (22), (21), (21), (21), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (17), (17), (15), (15), (13), (13), (12), (11), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1386), (502), (325), (36), (34), (15), (13), (8), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(1006), (523), (38), (16), (13), (5), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HER2 antibody is conjugated to PE
(76), (66), (59), (54), (47), (41), (21), (21), (14), (14), (13), (12), (12), (9), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
(721), (602), (333), (321), (309), (308), (269), (134), (112), (102), (91), (22), (21), (19), (7), (6), (6), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling Images
Specificity Human ErbB2 / HER2 / CD340
No cross-reactivity in ELISA with
Rat ErbB2
No Cross-Reactivity Rat (Rattus)
Characteristics This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human ErbB2 / HER2 extracellular domain (rh ErbB2, ABIN2001861 NP_004439.2, Met 1-Thr 652, and conjugated with PE under optimum conditions, the unreacted PE was removed.
Immunogen Recombinant Human ErbB2 protein (ABIN2001861)
Clone 511
Isotype IgG

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ERBB2 (ERBB2 Antibody Abstract)
Background Receptor tyrosine-protein kinase erbB-2, also known as epidermal growth factor receptor- related protein, proto-oncogene Neu, proto-oncogene c-ErbB-2, CD340 and ErbB2, is a single-pass type I membrane protein, cytoplasm protein and nucleus protein which belongs to the protein kinase superfamily, Tyr protein kinase family and EGF receptor subfamily. The ErbB protein family, also known as epidermal growth factor receptor (EGFR) family, is a family of four structurally related receptor tyrosine kinases (ErbB1/EGFR, ErbB2/HER2, ErbB3/HER3, ErbB4/HER4). The gene symbol, ErbB, is derived from the name of a viral oncogene to which these receptors are homologous: Erythroblastic Leukemia Viral Oncogene. Excessive ErbB signaling is associated with the development of a wide variety of types of solid tumor. Insufficient ErbB signaling in humans is associated with the development of neurodegenerative diseases, such as multiple sclerosis and Alzheimer's Disease. Mouse ErbB2/HER2 is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for rat ErbB2/HER2. It binds to the 5'-TCAAATTC-3' sequence in the MT-CO2 promoter and activates its transcription. ErbB2/HER2 is widely expressed in epithelial cells, and amplification and/or overexpression of ErbB2/HER2 has been reported associated with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate and ovarian cancers. ErbB2/HER2 cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. This receptor forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. ErbB2/HER2 mediates signalling pathways which involve mitogen-activated protein kinase and phosphatidylinositol- 3 kinase, this receptor plays an key role in development, cell proliferation and differentiation.
NCBI Accession NP_004439.2
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling Images back to top
Application Notes ICC/IF: 5-20 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details Images back to top
Format Liquid
Concentration 0.1 mg/mL
Buffer Aqueous solution containing 0.5 % BSA and 0.1 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal
Storage 4 °C
Storage Comment This antibody is stable for 12 months from date of receipt when stored at 2°C-8°C. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Expiry Date 12 months

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 1-652), (Extracellular Domain) antibody (PE) (ABIN1995358) Analysis of anti-human ErbB2 on MCF-7 cells. MCF-7 cells were detached using 1X tryps...
Immunofluorescence (IF) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 1-652), (Extracellular Domain) antibody (PE) (ABIN1995358) Immunofluorescence staining of Human ErbB2 in MCF7 cells. Cells were fixed with 4% PF...