You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human HER2 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • c-erbB2
  • c-neu
  • CD340
  • Erbb-2
  • HER-2
  • HER-2/neu
  • HER2
  • mKIAA3023
  • MLN 19
  • NEU
  • Neu
  • NGL
  • TKR1
Epitope
Extracellular Domain
120
83
64
54
53
53
51
44
37
36
32
29
21
20
20
20
20
19
19
19
19
19
19
18
18
18
15
14
14
14
13
13
13
12
10
10
9
8
8
8
8
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1322
418
261
24
13
4
3
3
3
3
2
2
1
1
1
Host
Rabbit
968
496
25
18
5
5
Clonality
Monoclonal
Conjugate
This HER2 antibody is un-conjugated
74
65
60
52
45
41
17
17
10
10
9
8
8
8
8
8
8
7
7
7
7
7
7
7
7
7
7
2
2
2
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
681
574
331
323
302
281
234
174
104
90
86
21
17
6
6
5
5
4
3
3
2
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN1019910
$ 292.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008700421 ABIN1019911 IHC (f) IHC (p) Rabbit IgG Extracellular Domain Log in to see SP3
0.0008700421 ABIN2688859 IHC (f) IHC (fro) FACS IP Mouse IgG1 Log in to see 9G6 7

Top referenced anti-HER2 antibody for Immunohistochemistry (Formalin-fixed Sections)

Similar anti-HER2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 192 Antibodies
ELISA 439 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 275 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 86 Antibodies
Immunoelectron Microscopy (IEM) 6 Antibodies
Immunofluorescence (IF) 302 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 90 Antibodies
Immunohistochemistry (IHC) 329 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 5 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 104 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 235 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope Extracellular Domain
(120), (83), (64), (54), (53), (53), (51), (44), (37), (36), (32), (29), (21), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (18), (18), (18), (15), (14), (14), (14), (13), (13), (13), (12), (10), (10), (9), (8), (8), (8), (8), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1322), (418), (261), (24), (13), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(968), (496), (25), (18), (5), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HER2 antibody is un-conjugated
(74), (65), (60), (52), (45), (41), (17), (17), (10), (10), (9), (8), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (2), (2), (2)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(681), (574), (331), (323), (302), (281), (234), (174), (104), (90), (86), (21), (17), (6), (6), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling Images
Purpose This antibody is designed for the specific localization of human c-erbB-2 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections. This antibody is recommended to determine the levels of expression of the oncoprotein c-erbB-2 in adenocarcinomas of breast, lung and other locations and transitional cell carcinomas of the urinary tract. Immunohistochemical staining of this oncoprotein is associated with gene amplification. In the case of breast cancer, overexpression of this oncoprotein has been shown to be associated with a worse prognosis.
Characteristics The gene product HER-2/neu (also known as c-erbB-2 gene product, p185 or CD380) belongs to the protein family of epidermal growth factor receptors. It is a 185 kDa transmembrane glycoprotein with tyrosine kinase activity. Some adenocarcinomas including carcinomas of the gastrointestinal tract and ovarian carcinomas as well as up to 30% of all breast carcinomas are showing an overexpression of HER2. It was shown that overexpression of HER2 is correlated with bad prognosis. Similar observations were made for osteosarcomas as well as stomach and bladder carcinomas. Proof of HER2 overexpression in breast carcinoma is regarded to be necessary for a therapy with Trastuzumab (Herceptin™). The antibody clone SP3 is directed against the extracellular domain of the HER2 protein. Immunohistochemistry (IHC) is a complex technique in which immunological and histological detection methods are combined. In general, the manipulation and processing of tissues before immunostaining, especially different types of tissue fixation and embedding, as well as the nature of the tissues themselves may cause inconsistent results (Nadji and Morales, 1983). Endogenous pseudoperoxidase and peroxidase activity or endogenous biotin and alkaline phosphatase activity can cause non-specific staining results depending on the detection system used. Tissues that contain Hepatitis B surface antigen (HBsAg) can produce false positives when using HRP detection systems (Omata et al, 1980). Insufficient contrast staining and/or improper mounting of the sample may influence the interpretation of results.
Purification Ready-to-use, Prediluted, obtained from culture supernatant
Material not included All reagents, materials, and laboratory equipment for IHC procedures are not provided with this antibody. This includes adhesive slides and cover slips, positive and negative control tissues, Xylene or adequate substitute, ethanol, distilled H2O, heat pretreatment equipment (pressure cooker, steamer, microwave), pipettes, Coplin jars, glass jars, moist chamber, histological baths, negative control reagents, counter-staining solution, mounting materials, and microscope. Buffered solutions for antigen retrieval, enzyme treatments, highly sensitive detection systems, and other auxiliary Anti- c-erbB-2/HER2/neu (
Immunogen Recombinant protein corresponding to the extracellular domain of human HER2 protein.
Clone SP3
Isotype IgG

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name c-erbB-2 (ERBB2 Antibody Abstract)
Research Area Receptors, Growth Factors
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling Images back to top
Comment

Staining pattern: Cell membrane. The interpretation of the stain results is the full responsibility of the user. Any experimental result must be confirmed by a medically established diagnostic product or procedure.
Positive control: Tissue sample from breast carcinoma with overexpression of the oncoprotein.
External Negative Control: Tissue sample homologous to the test sample incubated with an antibody isotype not specific for c-erbB-2.

Protocol Principles of the procedure: The demonstrations of antigens by IHC is a sequential procedure with several steps involving first the application of a specific antibody for the antigen of interest (primary antibody), then a secondary antibody which joins to the first, an enzyme complex, and the addition of a chromogenic substrate. The sample is washed between each step. Enzymatic activation of the chromogenic substrate creates a visible product where the antigen is located. The results are interpreted using a light microscope. The primary antibody can be used both in manual IHC and with automated immunostainers.

Paraffin-embedded tissue samples should be used. The antibody is also useful for immunostaining frozen tissue samples. Western blot techniques are not recommended.
Assay Procedure Antigen retrieval: HIER Citrate Buffer pH 6.5
Working dilution: (only for concentrates) 1:50 – 1:200
Incubation: 30 min, RT
Control Tissue: Breast carcinoma with overexpression of c-erbB-2
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details Images back to top
Format Liquid
Buffer The preparation contains saline buffer, stabilising and carriers proteins. (pH 7.4)
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2-8 °C until the expiration date printed on product label. Do not use after the expiration date. If fresh solutions are required, these must be prepared immediately prior to use, and will be stable for at least one day at room temperature (20-25°C). Unused portion of antibody preparation should be discarded after one day.If the product is stored under different conditions from those stipulated in these technical indications, the new conditions must be verified by the user. The validity period of the ready to use products when opened, is the same as the expiration date indicated on the label of intact product.