You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human HER2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • ERBB2
  • Erbb2
Epitope
AA 30-59, N-Term
100
82
70
63
56
53
52
46
44
35
27
23
21
21
21
21
21
20
20
20
20
20
20
19
19
19
18
16
15
14
13
13
12
11
10
8
8
8
8
8
7
7
7
6
6
6
5
5
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1354
499
333
35
34
15
13
8
6
5
4
3
3
3
2
1
1
1
1
1
Host
Rabbit
995
503
29
17
10
5
1
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
73
64
60
54
45
41
18
18
10
10
10
9
9
8
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
705
609
327
322
308
297
239
128
111
102
87
21
16
14
6
5
5
5
5
3
3
3
3
2
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN359840
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.931439 ABIN359843 EIA IHC (p) Rabbit Ig Log in to see Polyclonal 4
12.931439 ABIN499802 EIA IHC (p) WB Chicken IgG C-Term Log in to see Polyclonal 5
12.931439 ABIN967459 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 1242-1255 Log in to see 3B5 9
12.931439 ABIN4309121 ICC IF IHC IHC (p) WB Rabbit pTyr1248 Log in to see Polyclonal 1
12.931439 ABIN151232 IHC (p) IP IHC WB Rabbit AA 975-1025 Log in to see Polyclonal 1
12.931439 ABIN4309125 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
12.931439 ABIN498015 IHC (p) Rabbit Extracellular Domain, N-Term Log in to see Polyclonal
12.931439 ABIN305740 IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal
12.931439 ABIN498415 IF IHC (p) WB Rabbit Log in to see Polyclonal
12.931439 ABIN4309139 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HRB2-451
12.931439 ABIN295912 ICC IHC (p) WB Chicken IgY AA 153-433 Log in to see Polyclonal
12.931439 ABIN498847 IHC (p) Rabbit pTyr1248 Log in to see Polyclonal
12.931439 ABIN498846 IHC (p) Rabbit pTyr877 Log in to see Polyclonal
12.931439 ABIN725345 IF (p) IHC (p) Rabbit IgG AA 1210-1255 Log in to see Polyclonal
12.931439 ABIN351500 IHC (p) WB Mouse IgG1, kappa C-Term Log in to see SPM495
12.931439 ABIN213613 IHC (p) Rabbit Cytoplasmic Domain Log in to see Polyclonal
12.931439 ABIN4309126 FACS ICC IF IHC IHC (p) WB Mouse IgG2a Log in to see 4F10
12.931439 ABIN498935 IHC (p) Rabbit Log in to see Polyclonal
12.931439 ABIN498936 IHC (p) Rabbit Log in to see Polyclonal
1 ABIN391945 FACS IHC (p) WB Rabbit Ig AA 21-52, N-Term Log in to see Polyclonal 4

Top referenced anti-HER2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-HER2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 14 Antibodies
Dot Blot (DB) 191 Antibodies
ELISA 469 Antibodies
ELISA (Capture) 3 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 288 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 87 Antibodies
Immunoelectron Microscopy (IEM) 5 Antibodies
Immunofluorescence (IF) 308 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 102 Antibodies
Immunohistochemistry (IHC) 324 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 5 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 30-59, N-Term
(100), (82), (70), (63), (56), (53), (52), (46), (44), (35), (27), (23), (21), (21), (21), (21), (21), (20), (20), (20), (20), (20), (20), (19), (19), (19), (18), (16), (15), (14), (13), (13), (12), (11), (10), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1354), (499), (333), (35), (34), (15), (13), (8), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(995), (503), (29), (17), (10), (5), (1)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(73), (64), (60), (54), (45), (41), (18), (18), (10), (10), (10), (9), (9), (8), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(705), (609), (327), (322), (308), (297), (239), (128), (111), (102), (87), (21), (16), (14), (6), (5), (5), (5), (5), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN359840) Images
Specificity This antibody recognizes Human HER2/ErbB2. Other species not tested.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen KLH conjugated synthetic peptide between 30~59 amino acids from the N-terminal region of Human HER2/ErbB2 . Genename: ERBB2
Isotype Ig

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 Antibody (ABIN359840) Images back to top
Antigen
Alternative Name CD340 / ERBB2 / HER2 (ERBB2 Antibody Abstract)
Background ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An Interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residuesSynonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2
Gene ID 2064, 9606
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 Antibody (ABIN359840) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 Antibody (ABIN359840) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % (W/V) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-HER2 Antibody (ABIN359840)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Background publications

Ehsani, Low, Wallace, Wu: "Characterization of a new allele of the human ERBB2 gene by allele-specific competition hybridization." in: Genomics, Vol. 15, Issue 2, pp. 426-9, 1993

Yamamoto, Ikawa, Akiyama, Semba, Nomura, Miyajima, Saito, Toyoshima: "Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor." in: Nature, Vol. 319, Issue 6050, pp. 230-4, 1986

Coussens, Yang-Feng, Liao, Chen, Gray, McGrath, Seeburg, Libermann, Schlessinger, Francke: "Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene." in: Science (New York, N.Y.), Vol. 230, Issue 4730, pp. 1132-9, 1985

Semba, Kamata, Toyoshima, Yamamoto: "A v-erbB-related protooncogene, c-erbB-2, is distinct from the c-erbB-1/epidermal growth factor-receptor gene and is amplified in a human salivary gland adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 19, pp. 6497-501, 1985

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN359840) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 30-59) (ABIN359840) Flow Cytometric analysis of MCF-7 cells using HER2/ErbB2 Antibody (N-term) Cat.-No AP...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 30-59) (ABIN359840) (LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the prima...
Western Blotting (WB) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 30-59) (ABIN359840) (LEFT)Western blot analysis of anti-ErbB2 Pab in SKBR-3 cell lysate. ErbB2 (arrow) wa...