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anti-Human HER2 antibody for Western Blotting

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • c-erbB2
  • c-neu
  • CD340
  • Erbb-2
  • HER-2
  • HER-2/neu
  • HER2
  • mKIAA3023
  • MLN 19
  • NEU
  • Neu
  • NGL
  • TKR1
Epitope
AA 21-52, N-Term
116
82
64
54
54
51
51
44
36
32
32
28
21
20
20
20
20
19
19
19
19
19
19
18
18
18
15
14
14
14
13
13
13
12
10
10
9
8
8
8
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1145
407
234
24
12
4
3
3
3
3
2
2
1
1
1
Host
Rabbit
936
351
19
18
5
5
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
67
62
53
45
45
41
9
9
8
8
8
8
8
8
8
2
2
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
623
514
323
319
215
181
132
112
90
45
21
17
12
6
6
5
5
5
3
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN391945
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN391947 IHC (p) WB Rabbit Ig AA 1227-1255, C-Term, Tyr1248 Log in to see Polyclonal 4
0.12303223 ABIN359842 EIA WB Rabbit Ig Tyr1248 Log in to see Polyclonal 3
0.12303223 ABIN359840 EIA FACS IHC (p) WB Rabbit Ig AA 30-59, N-Term Log in to see Polyclonal 4
0.12303223 ABIN1532014 ELISA WB Rabbit IgG pTyr1112 Log in to see Polyclonal 2
0.12303223 ABIN1534423 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12303223 ABIN1533053 ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.12303223 ABIN1532623 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.12303223 ABIN391948 FACS IF IHC (p) WB Rabbit Ig Log in to see Polyclonal 4
0.12303223 ABIN499802 EIA IHC (p) WB Chicken IgG C-Term Log in to see Polyclonal 5
0.12303223 ABIN969111 FACS IHC ELISA WB Mouse IgG1 AA 750-987 Log in to see 6C2 2
0.12303223 ABIN967787 IF IP IHC WB Mouse IgG2b AA 182-373 Log in to see 42-c-erbB 5
0.12303223 ABIN967786 IF IP IHC WB Mouse IgG2b AA 182-373 Log in to see 42-c-erbB 5
0.12303223 ABIN1724703 IHC ELISA WB Mouse IgG1, IgG2b AA 750-987 Log in to see 6C2B12 1
0.12303223 ABIN969191 IHC ELISA WB Mouse IgG2b Log in to see 9B9D8 3
0.12303223 ABIN126789 IF WB Mouse IgG1 pTyr1112 Log in to see 19G5
0.12303223 ABIN126788 WB Mouse IgG1 AA 860-880 Log in to see 24B5
0.12303223 ABIN126790 EIA IF WB Mouse IgG1 pSer1113 Log in to see 9-00E-010
0.12303223 ABIN126791 WB Mouse IgG1 AA 1240-1260 Log in to see 19D2
0.12303223 ABIN1881313 DB IF WB Rabbit pTyr1196 Log in to see Polyclonal 3
0.12303223 ABIN1881312 DB IF WB Rabbit pTyr1005 Log in to see Polyclonal 3

Top referenced anti-HER2 antibody for Western Blotting

Similar anti-HER2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 192 Antibodies
ELISA 379 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 133 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 12 Antibodies
Immunoelectron Microscopy (IEM) 6 Antibodies
Immunofluorescence (IF) 215 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 90 Antibodies
Immunohistochemistry (IHC) 317 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 5 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 45 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 182 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 21-52, N-Term
(116), (82), (64), (54), (54), (51), (51), (44), (36), (32), (32), (28), (21), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (18), (18), (18), (15), (14), (14), (14), (13), (13), (13), (12), (10), (10), (9), (8), (8), (8), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1145), (407), (234), (24), (12), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(936), (351), (19), (18), (5), (5)
Clonality (Clone)
Polyclonal   ( )
Conjugate This HER2 antibody is un-conjugated
(67), (62), (53), (45), (45), (41), (9), (9), (8), (8), (8), (8), (8), (8), (8), (2), (2), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(623), (514), (323), (319), (215), (181), (132), (112), (90), (45), (21), (17), (12), (6), (6), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN391945) Images
Specificity This ErbB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30~59 amino acids from the N-terminal region of human HER2/ErbB2.
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This ERBB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-52 AA from the N-terminal region of human ERBB2.
Clone RB01464
Isotype Ig

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 Antibody (ABIN391945) Images back to top
Antigen
Alternative Name ErbB2 (ERBB2 Antibody Abstract)
Background ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An Interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residues
Synonyms: Tyrosine kinase-type cell surface receptor HER2, ERBB2, MLN 19
Pathways: Pathways in cancer, Non-small cell lung cancer, Calcium signaling pathway, Focal adhesion, Prostate cancer, Adherens junction, Bladder cancer, Pancreatic cancer, ErbB signaling pathway, Endometrial cancer
Molecular Weight 137909 Da
Gene ID 2064
UniProt P04626
Research Area Phospho-specific antibodies, Cell Signaling, Stem Cells, Cell Structure, Protein Modifications, Neurology
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 Antibody (ABIN391945) Images back to top
Application Notes WB = 1:1000, FACS = 1:10-50, IHC (p) = 1:50-100
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 Antibody (ABIN391945) Images back to top
Format Liquid
Concentration 1.28 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-HER2 Antibody (ABIN391945)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Background publications

Ehsani, Low, Wallace, Wu: "Characterization of a new allele of the human ERBB2 gene by allele-specific competition hybridization." in: Genomics, Vol. 15, Issue 2, pp. 426-9, 1993

Yamamoto, Ikawa, Akiyama, Semba, Nomura, Miyajima, Saito, Toyoshima: "Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor." in: Nature, Vol. 319, Issue 6050, pp. 230-4, 1986

Coussens, Yang-Feng, Liao, Chen, Gray, McGrath, Seeburg, Libermann, Schlessinger, Francke: "Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene." in: Science (New York, N.Y.), Vol. 230, Issue 4730, pp. 1132-9, 1985

Semba, Kamata, Toyoshima, Yamamoto: "A v-erbB-related protooncogene, c-erbB-2, is distinct from the c-erbB-1/epidermal growth factor-receptor gene and is amplified in a human salivary gland adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 19, pp. 6497-501, 1985

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN391945) back to top
Supplier Images
Western Blotting (WB) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 21-52) (ABIN391945) Western blot analysis of ErbB2 Pab (ABIN391945) in SKBR-3 cell lysate. ErbB2 (arrow) ...
Western Blotting (WB) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 21-52) (ABIN391945) Western blot analysis of ErbB2 (arrow) using HER2/ErbB2 Antibody (N-term) (ABIN391945...
Western Blotting (WB) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 21-52) (ABIN391945) Western blot analysis of ErbB2 (arrow) in T47D cell lysates, either noninduced (Lane ...