You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human HER2 antibody for Western Blotting

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • c-erbB2
  • c-neu
  • CD340
  • Erbb-2
  • HER-2
  • HER-2/neu
  • HER2
  • mKIAA3023
  • MLN 19
  • NEU
  • Neu
  • NGL
  • TKR1
Epitope
AA 1227-1255, C-Term, Tyr1248
115
84
66
56
53
51
49
45
44
34
33
29
23
20
20
20
20
19
19
19
19
19
19
18
18
18
16
16
14
14
14
14
12
12
12
10
9
9
8
8
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1327
424
261
24
13
4
3
3
3
3
2
2
1
1
1
Host
Rabbit
965
502
29
17
8
5
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
74
65
60
54
45
41
18
18
17
10
10
9
9
8
8
8
8
8
7
7
7
7
7
7
7
7
3
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
679
577
334
323
297
290
227
169
100
97
84
21
17
6
6
5
5
5
3
3
3
2
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN391947
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.11646428 ABIN391945 FACS IHC (p) WB Rabbit Ig AA 21-52, N-Term Log in to see Polyclonal 4
0.11646428 ABIN359840 EIA FACS IHC (p) WB Rabbit Ig AA 30-59, N-Term Log in to see Polyclonal 4
0.11646428 ABIN359842 EIA WB Rabbit Ig Tyr1248 Log in to see Polyclonal 3
0.11646428 ABIN1532014 ELISA WB Rabbit IgG pTyr1112 Log in to see Polyclonal 2
0.11646428 ABIN1534423 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.11646428 ABIN1532623 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.11646428 ABIN1533053 ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.11646428 ABIN391948 FACS IF IHC (p) WB Rabbit Ig Log in to see Polyclonal 4
0.11646428 ABIN499802 EIA IHC (p) WB Chicken IgG C-Term Log in to see Polyclonal 5
0.11646428 ABIN969111 FACS IHC ELISA WB Mouse IgG1 AA 750-987 Log in to see 6C2 2
0.11646428 ABIN967786 IF IP IHC WB Mouse IgG2b AA 182-373 Log in to see 42-c-erbB 5
0.11646428 ABIN967787 IF IP IHC WB Mouse IgG2b AA 182-373 Log in to see 42-c-erbB 5
0.11646428 ABIN126788 WB Mouse IgG1 AA 860-880 Log in to see 24B5
0.11646428 ABIN126789 IF WB Mouse IgG1 pTyr1112 Log in to see 19G5
0.11646428 ABIN126790 EIA IF WB Mouse IgG1 pSer1113 Log in to see 9-00E-010
0.11646428 ABIN126791 WB Mouse IgG1 AA 1240-1260 Log in to see 19D2
0.11646428 ABIN1881408 DB WB Rabbit pTyr1112 Log in to see Polyclonal 5
0.11646428 ABIN1881304 DB WB Rabbit pTyr1112 Log in to see Polyclonal 5
0.11646428 ABIN1881312 DB IF WB Rabbit pTyr1005 Log in to see Polyclonal 3
0.11646428 ABIN1881305 DB WB Rabbit pTyr1248 Log in to see Polyclonal 5

Top referenced anti-HER2 antibody for Western Blotting

Similar anti-HER2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 192 Antibodies
ELISA 442 Antibodies
ELISA (Capture) 3 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 280 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 84 Antibodies
Immunoelectron Microscopy (IEM) 6 Antibodies
Immunofluorescence (IF) 297 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 97 Antibodies
Immunohistochemistry (IHC) 331 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 5 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 100 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 226 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 1227-1255, C-Term, Tyr1248
(115), (84), (66), (56), (53), (51), (49), (45), (44), (34), (33), (29), (23), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (18), (18), (18), (16), (16), (14), (14), (14), (14), (12), (12), (12), (10), (9), (9), (8), (8), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1327), (424), (261), (24), (13), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(965), (502), (29), (17), (8), (5)
Clonality (Clone)
Polyclonal   ( )
Conjugate This HER2 antibody is un-conjugated
(74), (65), (60), (54), (45), (41), (18), (18), (17), (10), (10), (9), (9), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (3), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(679), (577), (334), (323), (297), (290), (227), (169), (100), (97), (84), (21), (17), (6), (6), (5), (5), (5), (3), (3), (3), (2), (2), (1), (1), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN391947) Images
Specificity This ErbB2 (Y1248) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y1248 of human ERBB2.
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This ERBB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1227-1255 AA from the C-terminal region of human ERBB2.
Clone RB15814
Isotype Ig

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 Antibody (ABIN391947) Images back to top
Antigen
Alternative Name ERBB2 (ERBB2 Antibody Abstract)
Background ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residues.
Synonyms: Tyrosine kinase-type cell surface receptor HER2, ERBB2
Molecular Weight 137909 DA
Gene ID 2064
UniProt P04626
Research Area Phospho-specific antibodies, Stem Cells, Protein Modifications, Neurology, Cell Structure
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 Antibody (ABIN391947) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 Antibody (ABIN391947) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-HER2 Antibody (ABIN391947)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Background publications

Ehsani, Low, Wallace, Wu: "Characterization of a new allele of the human ERBB2 gene by allele-specific competition hybridization." in: Genomics, Vol. 15, Issue 2, pp. 426-9, 1993

Yamamoto, Ikawa, Akiyama, Semba, Nomura, Miyajima, Saito, Toyoshima: "Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor." in: Nature, Vol. 319, Issue 6050, pp. 230-4, 1986

Coussens, Yang-Feng, Liao, Chen, Gray, McGrath, Seeburg, Libermann, Schlessinger, Francke: "Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene." in: Science (New York, N.Y.), Vol. 230, Issue 4730, pp. 1132-9, 1985

Semba, Kamata, Toyoshima, Yamamoto: "A v-erbB-related protooncogene, c-erbB-2, is distinct from the c-erbB-1/epidermal growth factor-receptor gene and is amplified in a human salivary gland adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 19, pp. 6497-501, 1985

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN391947) back to top
Supplier Images
Western Blotting (WB) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 1227-1255) (ABIN391947) Western blot analysis of ERBB2(arrow) using rabbit polyclonal ERBB2 Antibody (Y1248) ...
Western Blotting (WB) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 1227-1255) (ABIN391947) Western blot analysis of ERBB2 (arrow) in T47D cell lysates, either noninduced (Lane ...
Immunohistochemistry (IHC) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 1227-1255) (ABIN391947) Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Pho...