anti-Human HER2 antibody for Western Blotting

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • ERBB2
  • Erbb2
Epitope
AA 1227-1255, C-Term, Tyr1248
103
85
72
71
57
54
54
48
46
35
26
23
22
22
20
20
20
20
20
20
20
20
20
20
19
19
19
18
17
15
13
13
13
12
11
8
8
8
8
8
7
7
6
6
6
5
5
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1374
497
330
36
34
15
13
8
6
5
4
3
3
3
2
1
1
1
1
1
Host
Rabbit
1000
518
30
16
12
5
1
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
76
66
59
54
46
41
19
19
12
12
11
10
10
9
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
712
602
314
311
309
306
269
138
113
102
89
21
19
14
8
6
6
5
5
3
3
3
2
1
1
1
1
1
1
Options
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Catalog No. ABIN391947
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3487515 ABIN391945 FACS IHC (p) WB Rabbit Ig AA 21-52, N-Term Log in to see Polyclonal 4
3.3487515 ABIN359842 EIA WB Rabbit Ig Tyr1248 Log in to see Polyclonal 3
3.3487515 ABIN1532014 ELISA WB Rabbit IgG pTyr1112 Log in to see Polyclonal 2
3.3487515 ABIN391948 FACS IF IHC (p) WB Rabbit Ig Log in to see Polyclonal 4
3.3487515 ABIN1533053 ELISA WB Rabbit IgG Log in to see Polyclonal 2
3.3487515 ABIN1532623 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 2
3.3487515 ABIN1534423 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
3.3487515 ABIN967787 IF IP IHC WB Mouse IgG2b AA 182-373 Log in to see 42-c-erbB 5
3.3487515 ABIN967786 IF IP IHC WB Mouse IgG2b AA 182-373 Log in to see 42-c-erbB 5
3.3487515 ABIN4309121 ICC IF IHC IHC (p) WB Rabbit pTyr1248 Log in to see Polyclonal 2
3.3487515 ABIN969111 FACS IHC ELISA WB Mouse IgG1 AA 750-987 Log in to see 6C2 2
3.3487515 ABIN4309120 IHC IHC (p) IP WB Rabbit pTyr877 Log in to see Polyclonal 1
3.3487515 ABIN1881308 WB Rabbit pTyr1221 Log in to see Polyclonal 3
3.3487515 ABIN1881305 DB WB Rabbit pTyr1248 Log in to see Polyclonal 5
3.3487515 ABIN1881312 DB IF WB Rabbit pTyr1005 Log in to see Polyclonal 3
3.3487515 ABIN1881313 DB IF WB Rabbit pTyr1196 Log in to see Polyclonal 3
3.3487515 ABIN1881304 DB WB Rabbit pTyr1112 Log in to see Polyclonal 5
3.3487515 ABIN1881408 DB WB Rabbit pTyr1112 Log in to see Polyclonal 5
3.3487515 ABIN126791 WB Mouse IgG1 AA 1240-1260 Log in to see 19D2
3.3487515 ABIN126789 IF WB Mouse IgG1 pTyr1112 Log in to see 19G5

Top referenced anti-HER2 antibody for Western Blotting

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Application / Reactivity Human
Cell Culture (CC) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 14 Antibodies
Dot Blot (DB) 185 Antibodies
ELISA 459 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 19 Antibodies
Flow Cytometry (FACS) 296 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 89 Antibodies
Immunoelectron Microscopy (IEM) 6 Antibodies
Immunofluorescence (IF) 306 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 102 Antibodies
Immunohistochemistry (IHC) 308 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 1227-1255, C-Term, Tyr1248
(103), (85), (72), (71), (57), (54), (54), (48), (46), (35), (26), (23), (22), (22), (20), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (18), (17), (15), (13), (13), (13), (12), (11), (8), (8), (8), (8), (8), (7), (7), (6), (6), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1374), (497), (330), (36), (34), (15), (13), (8), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(1000), (518), (30), (16), (12), (5), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This HER2 antibody is un-conjugated
(76), (66), (59), (54), (46), (41), (19), (19), (12), (12), (11), (10), (10), (9), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(712), (602), (314), (311), (309), (306), (269), (138), (113), (102), (89), (21), (19), (14), (8), (6), (6), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling ProductDetails: References for anti-HER2 antibody (ABIN391947) Images
Specificity This ErbB2 (Y1248) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y1248 of human ERBB2.
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This ERBB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1227-1255 AA from the C-terminal region of human ERBB2.
Clone RB15814
Isotype Ig

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling ProductDetails: References for anti-HER2 antibody (ABIN391947) Images back to top
Antigen
Alternative Name ERBB2 (ERBB2 Antibody Abstract)
Background ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residues.
Synonyms: Tyrosine kinase-type cell surface receptor HER2, ERBB2
Molecular Weight 137909 DA
Gene ID 2064
UniProt P04626
Research Area Phospho-specific antibodies, Stem Cells, Protein Modifications, Neurology, Cell Structure
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling ProductDetails: References for anti-HER2 antibody (ABIN391947) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details ProductDetails: References for anti-HER2 antibody (ABIN391947) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

ProductDetails: References for anti-HER2 antibody (ABIN391947)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Background publications

Ehsani, Low, Wallace, Wu: "Characterization of a new allele of the human ERBB2 gene by allele-specific competition hybridization." in: Genomics, Vol. 15, Issue 2, pp. 426-9, 1993

Yamamoto, Ikawa, Akiyama, Semba, Nomura, Miyajima, Saito, Toyoshima: "Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor." in: Nature, Vol. 319, Issue 6050, pp. 230-4, 1986

Coussens, Yang-Feng, Liao, Chen, Gray, McGrath, Seeburg, Libermann, Schlessinger, Francke: "Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene." in: Science (New York, N.Y.), Vol. 230, Issue 4730, pp. 1132-9, 1985

Semba, Kamata, Toyoshima, Yamamoto: "A v-erbB-related protooncogene, c-erbB-2, is distinct from the c-erbB-1/epidermal growth factor-receptor gene and is amplified in a human salivary gland adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 19, pp. 6497-501, 1985

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling ProductDetails: References for anti-HER2 antibody (ABIN391947) back to top
Supplier Images
Western Blotting (WB) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 1227-1255) (ABIN391947) Western blot analysis of ERBB2(arrow) using rabbit polyclonal ERBB2 Antibody (Y1248) ...
Western Blotting (WB) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 1227-1255) (ABIN391947) Western blot analysis of ERBB2 (arrow) in T47D cell lysates, either noninduced (Lane ...
Immunohistochemistry (IHC) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 1227-1255) (ABIN391947) Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Pho...