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anti-Mouse (Murine) HER2 antibody for Immunoprecipitation

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • c-erbB2
  • c-neu
  • CD340
  • Erbb-2
  • HER-2
  • HER-2/neu
  • HER2
  • mKIAA3023
  • MLN 19
  • NEU
  • Neu
  • NGL
  • TKR1
Epitope
C-Term, pTyr1129
115
82
64
54
54
51
51
44
36
32
32
29
21
20
20
20
20
19
19
19
19
19
19
18
18
18
15
14
14
14
13
13
13
12
10
10
9
8
8
8
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1145
406
233
24
12
4
3
3
3
3
2
2
1
1
1
Host
Rabbit
936
351
19
18
5
5
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
67
62
53
45
45
41
9
9
8
8
8
8
8
8
8
2
2
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
623
514
323
319
215
182
133
111
90
45
21
17
12
6
6
5
5
5
3
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN493356
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008919076 ABIN181400 IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 1
0.0008919076 ABIN493049 IP WB Rabbit Log in to see Polyclonal 3
0.0008919076 ABIN372478 IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal
0.0008919076 ABIN318038 EIA IHC (p) IP WB Rabbit pTyr877 Log in to see Polyclonal
0.0008919076 ABIN318035 IHC (p) IP WB Rabbit pTyr1221, pTyr1222 Log in to see Polyclonal
0.0008919076 ABIN2704879 IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.0008919076 ABIN1584123 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0008919076 ABIN2470582 IP IHC WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal
0.0008919076 ABIN2164697 IP IHC ELISA WB Rabbit IgG Tyr877 Log in to see Polyclonal
0.0008919076 ABIN2755561 IC IF IP IHC WB Rabbit C-Term, pTyr1248 Log in to see Polyclonal
0.0008919076 ABIN1734112 IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal
0.0008919076 ABIN184456 IHC (fro) IHC (p) IP WB Rabbit Extracellular Domain Log in to see Polyclonal
0.0008919076 ABIN1846011 IP IHC WB Rabbit pTyr877 Log in to see Polyclonal
0.0008919076 ABIN2962674 FACS IHC (fro) IHC (p) IP WB Mouse IgG1 C-Term Log in to see 3B5
0.0008919076 ABIN1002431 IP WB Rabbit IgG Log in to see Polyclonal
0.0008919076 ABIN2749944 IP ELISA WB FITC Rabbit IgG AA 1100-1150, phosphorylated Log in to see Polyclonal
0.0008919076 ABIN2746730 IP ELISA WB FITC Rabbit IgG Log in to see Polyclonal
0.0008919076 ABIN1576967 IP IHC ELISA WB Rabbit pTyr877 Log in to see Polyclonal
0.0008919076 ABIN1847484 IP IHC ELISA WB Rabbit Internal Region, pTyr877, pTyr878, pTyr879 Log in to see Polyclonal
0.0008919076 ABIN2749943 IP ELISA WB Rabbit IgG AA 1100-1150, phosphorylated Log in to see Polyclonal

Top referenced anti-HER2 antibody for Immunoprecipitation

Similar anti-HER2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 603 Antibodies 192 Antibodies 142 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Luminex Assay (LMNX) 21 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 114 Antibodies 26 Antibodies 24 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 182 Antibodies 43 Antibodies 51 Antibodies
Immunohistochemistry (IHC) 317 Antibodies 88 Antibodies 84 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 45 Antibodies 4 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope C-Term, pTyr1129
(115), (82), (64), (54), (54), (51), (51), (44), (36), (32), (32), (29), (21), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (18), (18), (18), (15), (14), (14), (14), (13), (13), (13), (12), (10), (10), (9), (8), (8), (8), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1145), (406), (233), (24), (12), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(936), (351), (19), (18), (5), (5)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(67), (62), (53), (45), (45), (41), (9), (9), (8), (8), (8), (8), (8), (8), (8), (2), (2), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(623), (514), (323), (319), (215), (182), (133), (111), (90), (45), (21), (17), (12), (6), (6), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN493356) Images
Sequence ETD GY(p)V APL AC
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse and Rat.
Purification Affinity Chromatography.
Immunogen This antibodies were generated against a conserved sequence near the C-terminal that is unique to HER2 protein.

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 Antibody (ABIN493356) Images back to top
Antigen
Alternative Name CD340 / ERBB2 / HER2 (ERBB2 Antibody Abstract)
Background The tyrosine kinase receptor erbB2, also known in humans as Her2, is a member of the epidermal growth factor receptor (EGFR or erbB1) family, which also includes erbB3 and erbB4 that are gene products of proto-oncogenes. Protocogenes play an important role in normal proliferation of cells. The interaction of proto-oncogenes with other tumor suppressor proteins (P53, P14ARF etc.) determine the normal or abnormal course of cell cycle and cell division leading to uncontrolled cell proliferation (1). Her 2 is found on chromosome 17 and codes for a protein that serves as a cell surface receptor. Her protein family of receptor has 4 structurally similar members of membrane receptors that have intrinsic tyrosine kinase activity. These Her receptors working in concert, determine the regulation of cell growth and differentiation in a complex manner. Her-2 receptor is still considered to be an orphan receptor with no identifiable ligand. Her family receptors can form homo or hetero dimers with other compounds. The activation of these homo-heterodimmeric complexes by ligand binding led to an activation of intrinsic tyrosine kinase activity that triggers a cascade of signal transduction activity in cell. Some of these signals are transduced across the cell membranes in to cytoplasm and triggers the gene activation. A better understanding of these receptors and identification of their respective ligands will greatly enhance our understanding of cell cycle and uncontrolled cell division as seen in cancerous tissues. Several steps in these pathways are currently being targeted for therapeutic intervention, including tyrosine kinase inhibitors. There are at least 2 humanized recombinant monoclonal antibodies (Trastuzumab and pertuzumab) that target extra cellular regions of the Her 2 tyrosine kinase receptor and have been used in human trials for the treatment of breast cancer (2). Herceptin-2 is a 1342 amino acid protein (approximate MW 170-172 kDa) when analyzed on reduced SDS-PAGE gels. Her2 is over expressed in cells of many cancer types. It's over expression is nicely correlated with a poor prognosis of breast and ovarian cancer in patients. ErbB receptors bind to a family of growth factors, termed neuregulins/heregulin (NRG/HRG), which comprise NRG-1, -2, -3, and -4 and include multiple variants. ErbB2/Her2 is an orphan receptor that does not bind ligand alone but heterodimerizes with the other erbB receptors for NRG signaling. Her 2 is a receptor tyrosine kinase with intrinsic tyrosine kinase domain.Synonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2
Gene ID 2064, 10116
UniProt P06494
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 Antibody (ABIN493356) Images back to top
Application Notes This antibody is ideal for IMM and Western Blot. Recommended Dilutions: Western Blot: 1/500IMM and i. p pull-down assays: > 1/200 (1 μL/250 μg protein extracts).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 Antibody (ABIN493356) Images back to top
Concentration 0.65-0.75 mg/mL
Buffer Stabilization buffer with 0.02 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-HER2 Antibody (ABIN493356)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Background publications

Negro, Brar, Lee: "Essential roles of Her2/erbB2 in cardiac development and function." in: Recent progress in hormone research, Vol. 59, pp. 1-12, 2004

Nahta, Hung, Esteva: "The HER-2-targeting antibodies trastuzumab and pertuzumab synergistically inhibit the survival of breast cancer cells." in: Cancer research, Vol. 64, Issue 7, pp. 2343-6, 2004

DCruz, Yasumura, Weir, Matthes, Abderrahim, LaVail, Vollrath: "Mutation of the receptor tyrosine kinase gene Mertk in the retinal dystrophic RCS rat." in: Human molecular genetics, Vol. 9, Issue 4, pp. 645-51, 2000

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN493356) back to top
Supplier Images
 image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (C-Term) (ABIN493356) anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (C-Term), (pTyr1129) antibody