anti-Mouse (Murine) HER2 antibody for Immunoprecipitation

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • ERBB2
  • Erbb2
Epitope
C-Term, pTyr1129
103
82
72
70
56
54
53
48
46
35
26
24
22
22
20
20
20
20
20
20
20
20
20
19
19
19
19
18
17
15
13
13
13
12
11
8
8
8
8
8
7
7
6
6
6
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1348
495
329
36
34
15
13
8
6
5
4
3
3
3
2
1
1
1
1
1
Host
Rabbit
980
511
30
16
12
5
1
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
76
66
59
54
46
41
19
19
12
12
11
10
10
9
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Immunoprecipitation (IP), Western Blotting (WB)
701
596
311
310
309
297
248
137
110
102
89
21
19
14
8
6
6
5
5
3
3
3
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN493356
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN181400 IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 1
1 ABIN4309120 IHC IHC (p) IP WB Rabbit pTyr877 Log in to see Polyclonal 1
1 ABIN493049 IP WB Rabbit Log in to see Polyclonal 3
1 ABIN372478 IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal
1 ABIN305740 IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal
1 ABIN318038 EIA IHC (p) IP WB Rabbit pTyr877 Log in to see Polyclonal
1 ABIN318035 IHC (p) IP WB Rabbit pTyr1221, pTyr1222 Log in to see Polyclonal
1 ABIN2704879 IP IHC WB Rabbit C-Term Log in to see Polyclonal
1 ABIN959522 IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see 3B5
1 ABIN2450532 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2470582 IP IHC WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal
1 ABIN2888432 IHC (p) IP ELISA WB Rabbit IgG pTyr877 Log in to see Polyclonal
1 ABIN2888431 IHC (p) IP ELISA WB Rabbit IgG pTyr1221 Log in to see Polyclonal
1 ABIN1584123 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN385644 IHC (p) IP WB Mouse IgG1 Log in to see e2-4001
1 ABIN387008 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2962674 FACS IHC (fro) IHC (p) IP WB Mouse IgG1 C-Term Log in to see 3B5
1 ABIN1734112 IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal
1 ABIN386124 IHC IP WB Biotin Mouse IgG1 C-Term Log in to see 3B5
1 ABIN2755561 IC IF IP IHC WB Rabbit C-Term, pTyr1248 Log in to see Polyclonal

Top referenced anti-HER2 antibody for Immunoprecipitation

Similar anti-HER2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 8 Antibodies 2 Antibodies 2 Antibodies
Cytometry by Time of Flight (CyTOF) 14 Antibodies 2 Antibodies
Western Blotting (WB) 669 Antibodies 248 Antibodies 225 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies 1 Antibodies 1 Antibodies
Neutralization (Neut) 1 Antibodies
Luminex Assay (LMNX) 21 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 137 Antibodies 64 Antibodies 55 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope C-Term, pTyr1129
(103), (82), (72), (70), (56), (54), (53), (48), (46), (35), (26), (24), (22), (22), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (18), (17), (15), (13), (13), (13), (12), (11), (8), (8), (8), (8), (8), (7), (7), (6), (6), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1348), (495), (329), (36), (34), (15), (13), (8), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(980), (511), (30), (16), (12), (5), (1)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(76), (66), (59), (54), (46), (41), (19), (19), (12), (12), (11), (10), (10), (9), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application Immunoprecipitation (IP), Western Blotting (WB)
(701), (596), (311), (310), (309), (297), (248), (137), (110), (102), (89), (21), (19), (14), (8), (6), (6), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling ProductDetails: References for anti-HER2 antibody (ABIN493356) Images
Sequence ETD GY(p)V APL AC
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse and Rat.
Purification Affinity Chromatography.
Immunogen This antibodies were generated against a conserved sequence near the C-terminal that is unique to HER2 protein.

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling ProductDetails: References for anti-HER2 antibody (ABIN493356) Images back to top
Antigen
Alternative Name CD340 / ERBB2 / HER2 (ERBB2 Antibody Abstract)
Background The tyrosine kinase receptor erbB2, also known in humans as Her2, is a member of the epidermal growth factor receptor (EGFR or erbB1) family, which also includes erbB3 and erbB4 that are gene products of proto-oncogenes. Protocogenes play an important role in normal proliferation of cells. The interaction of proto-oncogenes with other tumor suppressor proteins (P53, P14ARF etc.) determine the normal or abnormal course of cell cycle and cell division leading to uncontrolled cell proliferation (1). Her 2 is found on chromosome 17 and codes for a protein that serves as a cell surface receptor. Her protein family of receptor has 4 structurally similar members of membrane receptors that have intrinsic tyrosine kinase activity. These Her receptors working in concert, determine the regulation of cell growth and differentiation in a complex manner. Her-2 receptor is still considered to be an orphan receptor with no identifiable ligand. Her family receptors can form homo or hetero dimers with other compounds. The activation of these homo-heterodimmeric complexes by ligand binding led to an activation of intrinsic tyrosine kinase activity that triggers a cascade of signal transduction activity in cell. Some of these signals are transduced across the cell membranes in to cytoplasm and triggers the gene activation. A better understanding of these receptors and identification of their respective ligands will greatly enhance our understanding of cell cycle and uncontrolled cell division as seen in cancerous tissues. Several steps in these pathways are currently being targeted for therapeutic intervention, including tyrosine kinase inhibitors. There are at least 2 humanized recombinant monoclonal antibodies (Trastuzumab and pertuzumab) that target extra cellular regions of the Her 2 tyrosine kinase receptor and have been used in human trials for the treatment of breast cancer (2). Herceptin-2 is a 1342 amino acid protein (approximate MW 170-172 kDa) when analyzed on reduced SDS-PAGE gels. Her2 is over expressed in cells of many cancer types. It's over expression is nicely correlated with a poor prognosis of breast and ovarian cancer in patients. ErbB receptors bind to a family of growth factors, termed neuregulins/heregulin (NRG/HRG), which comprise NRG-1, -2, -3, and -4 and include multiple variants. ErbB2/Her2 is an orphan receptor that does not bind ligand alone but heterodimerizes with the other erbB receptors for NRG signaling. Her 2 is a receptor tyrosine kinase with intrinsic tyrosine kinase domain.Synonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2
Gene ID 2064, 10116
UniProt P06494
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling ProductDetails: References for anti-HER2 antibody (ABIN493356) Images back to top
Application Notes This antibody is ideal for IMM and Western Blot. Recommended Dilutions: Western Blot: 1/500IMM and i. p pull-down assays: > 1/200 (1 μL/250 μg protein extracts).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details ProductDetails: References for anti-HER2 antibody (ABIN493356) Images back to top
Concentration 0.65-0.75 mg/mL
Buffer Stabilization buffer with 0.02 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.

ProductDetails: References for anti-HER2 antibody (ABIN493356)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Background publications

Negro, Brar, Lee: "Essential roles of Her2/erbB2 in cardiac development and function." in: Recent progress in hormone research, Vol. 59, pp. 1-12, 2004

Nahta, Hung, Esteva: "The HER-2-targeting antibodies trastuzumab and pertuzumab synergistically inhibit the survival of breast cancer cells." in: Cancer research, Vol. 64, Issue 7, pp. 2343-6, 2004

DCruz, Yasumura, Weir, Matthes, Abderrahim, LaVail, Vollrath: "Mutation of the receptor tyrosine kinase gene Mertk in the retinal dystrophic RCS rat." in: Human molecular genetics, Vol. 9, Issue 4, pp. 645-51, 2000

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling ProductDetails: References for anti-HER2 antibody (ABIN493356) back to top
Supplier Images
 image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (C-Term) (ABIN493356) anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (C-Term), (pTyr1129) antibody