anti-Rat (Rattus) HER2 antibody for Immunohistochemistry (Frozen Sections)

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • ERBB2
  • Erbb2
Epitope
AA 1243-1255
101
95
72
69
61
58
55
55
48
35
26
22
22
21
21
20
20
20
20
20
20
20
20
20
19
19
19
17
17
15
15
13
13
12
11
8
8
8
8
8
7
7
6
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
1386
501
324
35
33
15
12
7
5
5
4
3
3
3
2
1
1
1
1
1
Host
Rabbit
1006
523
38
16
13
5
1
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
76
66
59
55
47
41
21
21
14
14
13
12
12
9
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
720
602
334
321
309
309
268
133
111
102
91
22
21
19
8
6
6
5
5
3
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN305740
$ 451.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN372478 IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal
1 ABIN959522 IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see 3B5
1 ABIN1734112 IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal
1 ABIN576254 IHC (fro) ELISA WB Rabbit IgG pThr686 Log in to see Polyclonal
1 ABIN4309180 ELISA FACS ICC IF IHC (fro) WB Alexa Fluor 405 Mouse IgG2a kappa Log in to see HRB2-258
1 ABIN4309183 ELISA FACS ICC IF IHC (fro) WB Alexa Fluor 700 Mouse IgG2a kappa Log in to see HRB2-258
1 ABIN4309181 ELISA FACS ICC IF IHC (fro) WB Alexa Fluor 488 Mouse IgG2a kappa Log in to see HRB2-258
1 ABIN4309182 ELISA FACS ICC IF IHC (fro) WB Alexa Fluor 647 Mouse IgG2a kappa Log in to see HRB2-258
1 ABIN4309186 ELISA FACS ICC IF IHC (fro) WB DyLight 650 Mouse IgG2a kappa Log in to see HRB2-258
1 ABIN4309185 ELISA ICC IF IHC (fro) WB Biotin Mouse IgG2a kappa Log in to see HRB2-258
1 ABIN4309193 ELISA FACS ICC IF IHC (fro) WB DyLight 550 Mouse IgG2a kappa Log in to see HRB2-258
1 ABIN4309194 ELISA FACS ICC IF IHC (fro) WB DyLight 350 Mouse IgG2a kappa Log in to see HRB2-258
1 ABIN4309190 ELISA FACS ICC IF IHC (fro) WB DyLight 755 Mouse IgG2a kappa Log in to see HRB2-258
1 ABIN4309187 ELISA FACS ICC IF IHC (fro) WB DyLight 680 Mouse IgG2a kappa Log in to see HRB2-258
1 ABIN4309191 FACS ICC IF IHC (fro) IP WB PerCP Mouse IgG2a kappa Log in to see HRB2-258
1 ABIN4309195 ELISA FACS ICC IF IHC (fro) WB DyLight 405 Mouse IgG2a kappa Log in to see HRB2-258
1 ABIN2962674 FACS IHC (fro) IHC (p) IP WB Mouse IgG1 C-Term Log in to see 3B5
1 ABIN4309188 ELISA FACS ICC IF IHC (fro) WB DyLight 488 Mouse IgG2a kappa Log in to see HRB2-258
1 ABIN4309175 CyTOF ELISA FACS ICC IF IHC (fro) IP WB Mouse IgG2a kappa Log in to see HRB2-258
1 ABIN4309174 CyTOF ELISA FACS ICC IF IHC (fro) IP WB Mouse IgG2a kappa Log in to see HRB2-258

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 1243-1255
(101), (95), (72), (69), (61), (58), (55), (55), (48), (35), (26), (22), (22), (21), (21), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (17), (17), (15), (15), (13), (13), (12), (11), (8), (8), (8), (8), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(1386), (501), (324), (35), (33), (15), (12), (7), (5), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(1006), (523), (38), (16), (13), (5), (1)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(76), (66), (59), (55), (47), (41), (21), (21), (14), (14), (13), (12), (12), (9), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(720), (602), (334), (321), (309), (309), (268), (133), (111), (102), (91), (22), (21), (19), (8), (6), (6), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling Images
Brand IHC-plus™
Specificity Recognizes c-erbB-2 (185kD), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (100%) Elephant, Bat, Bovine (92%) Opossum (85%).
Purification Protein A purified
Immunogen A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa1243-1255 from the human c-erbB-2/HER-2/neu oncoprotein. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (100%), Elephant, Bat, Bovine (92%), Opossum (85%).
Isotype IgG

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ERBB2 / HER2 (ERBB2 Antibody Abstract)
Background Name/Gene ID: ERBB2
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: ERBB2, C-erb B2/neu protein, CD340, HER2, Herstatin, MLN 19, HER-2, Neu, NGL, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, CD340 antigen, MLN19, p185erbB2, TKR1
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling Images back to top
Application Notes Approved: IHC-Fr, IHC-P (5 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL detects c-erbB-2 in RIPA lysates from A431, MDA, MCF-7, LNCap, and c-erbB-2 transfected 3T3/NIH cells. Immunoprecipitation: 4 μg immunoprecipitated c-erbB-2 from 500 μg of A431, LNCap, and c-erbB- 2 transfected 3T3/NIH RIPA lysate.
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 10 mM PBS, pH 7.4, 0.2 % BSA, 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 1243-1255) antibody (ABIN305740) Human Breast, Carcinoma (formalin-fixed, paraffin-embedded) stained with ERBB2 antibo...