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Human HER2 ELISA Kit

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Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) ELISA Kits
  • c-erbB2
  • c-neu
  • CD340
  • Erbb-2
  • HER-2
  • HER-2/neu
  • HER2
  • mKIAA3023
  • MLN 19
  • NEU
  • Neu
  • NGL
  • TKR1
Epitope
AA 23-652
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
33
15
15
1
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
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Catalog No. ABIN921095
$ 400.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.3092964 ABIN579058 Cell Culture Supernatant, Serum, Plasma, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Log in to see
0.3092964 ABIN2708239 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.3092964 ABIN2113832 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin), Tissue Lysate Log in to see
0.001212079 ABIN3032917 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 8.2-2000 pg/mL 8.2 pg/mL Log in to see
0.001212079 ABIN625296 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 1
0.001212079 ABIN1380599 Cell ELISA Log in to see
0.001212079 ABIN1380598 Cell ELISA Log in to see
0.001212079 ABIN1380600 Cell ELISA Log in to see
0.001212079 ABIN1380748 Cell ELISA Log in to see
0.001212079 ABIN1112598 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.001212079 ABIN1381199 Cell ELISA Log in to see
0.001212079 ABIN2042457 Competition ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.001212079 ABIN2684155 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Amniotic Fluid, Cell Culture Supernatant, Serum 0.16-10 ng/mL 0.16 ng/mL Log in to see
0.001212079 ABIN2684157 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.001212079 ABIN2684156 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum, Tissue Lysate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.001212079 ABIN2114835 Cell ELISA Log in to see
0.001212079 ABIN2114832 Cell ELISA Log in to see
0.001212079 ABIN2114833 Cell ELISA Log in to see
0.001212079 ABIN2114834 Cell ELISA Log in to see
0.001212079 ABIN2113218 Cell ELISA Log in to see

Top referenced HER2 ELISA Kit for ELISA

Similar HER2 ELISA Kits

Application / Reactivity Human
ELISA 33 ELISA Kits

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) ELISA Kits
Epitope AA 23-652
Reactivity Human
Kits with alternative reactivity to:
(33), (15), (15), (1)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details HER2 ELISA Kit

Target details Application Details Handling References for HER2 Kit (ABIN921095) Images
Purpose For quantitative detection of human ErbB-2 in cell culture supernates, serum, plasma(heparin, EDTA) and tissue homogenates.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate
Detection Method Colorimetric
Specificity Natural and recombinant human ErbB-2
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human ErbB-2 antibod - 1
  • Lyophilized recombinant human ErbB-2 standar - 10ng/tubex2
  • Biotinylated anti- human ErbB-2 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: T23-T652

Target details

Product Details HER2 ELISA Kit Application Details Handling References for HER2 Kit (ABIN921095) Images back to top
Antigen
Alternative Name ERBB2 (ERBB2 ELISA Kit Abstract)
Background HER2/neu(also known as ErbB-2) stands for "Human Epidermal growth factor Receptor 2" and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2/neu has also been designated as CD340(cluster of differentiation 340) and p185. It is encoded by the ERBB2 gene.HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. It is encoded within the genome by HER2/neu, a known proto-oncogene. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. However, ErbB receptors dimerise on ligand binding, and HER2 is the preferential dimerisation partner of other members of the ErbB family.1 The HER2 gene is a proto-oncogene located at the long arm of human chromosome 17(17q21-q22)2.
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details HER2 ELISA Kit Target details Handling References for HER2 Kit (ABIN921095) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human ErbB-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ErbB-2 has been precoated onto 96-well plates. Standards(NSO, T23-T652) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ErbB-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ErbB-2 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human ErbB-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum, plasma(heparin, EDTA) or tissue lysates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ErbB-2 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 348, Standard deviation: 14.62, CV(%): 4.2
  • Sample 2: n=16, Mean(pg/ml): 1057, Standard deviation: 45.45, CV(%): 4.3
  • Sample 3: n=16, Mean(pg/ml): 2334, Standard deviation: 91.03, CV(%): 3.9,
  • Sample 1: n=24, Mean(pg/ml): 361, Standard deviation: 21.3, CV(%): 5.9
  • Sample 2: n=24, Mean(pg/ml): 1049, Standard deviation: 67.14, CV(%): 6.4
  • Sample 3: n=24, Mean(pg/ml): 2645, Standard deviation: 140.2, CV(%): 5.3
Restrictions For Research Use only

Handling

Product Details HER2 ELISA Kit Target details Application Details References for HER2 Kit (ABIN921095) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for HER2 Kit (ABIN921095)

Product Details HER2 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Tao, Suhua, Juanjuan, Zongzhi, Juan, Dandan: "In vitro study on human cytomegalovirus affecting early pregnancy villous EVT's invasion function." in: Virology journal, Vol. 8, pp. 114, 2011

Images

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Supplier Images
ELISA image for HER2 ELISA Kit (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (ABIN921095) Human ErbB-2 PicoKine ELISA Kit standard curve