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Human HER2 ELISA Kit

Recommended HER2 Product (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) ELISA Kits
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • ERBB2
  • Erbb2
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
32
15
15
1
1
Methode Type
Sandwich ELISA
Detection Range
8-2000 pg/mL
Minimum Detection Limit
8 pg/mL
Application
ELISA
Options
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Catalog No. ABIN625296
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.840559 ABIN921095 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see 1
10.840559 ABIN579058 Cell Culture Supernatant, Serum, Plasma, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Log in to see
10.840559 ABIN2708239 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
10.840559 ABIN2113832 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin), Tissue Lysate Log in to see
1 ABIN3032917 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 8.2-2000 pg/mL 8.2 pg/mL Log in to see
1 ABIN1112598 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN1380599 Cell ELISA Log in to see
1 ABIN1380598 Cell ELISA Log in to see
1 ABIN1380600 Cell ELISA Log in to see
1 ABIN1380748 Cell ELISA Log in to see
1 ABIN1381199 Cell ELISA Log in to see
1 ABIN4886083 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN2042457 Competition ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN2684157 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN2684156 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum, Tissue Lysate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN2114833 Cell ELISA Log in to see
1 ABIN2114832 Cell ELISA Log in to see
1 ABIN2114834 Cell ELISA Log in to see
1 ABIN2114835 Cell ELISA Log in to see
1 ABIN1846908 Tissue Homogenate, Cell Lysate, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see

Top referenced HER2 ELISA Kit for ELISA

Similar HER2 ELISA Kits

Application / Reactivity Human
ELISA 33 ELISA Kits

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(32), (15), (15), (1), (1)
Methode Type Sandwich ELISA
Detection Range 8-2000 pg/mL
Minimum Detection Limit 8 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details HER2 ELISA Kit

Target details Application Details Handling References for HER2 Kit (ABIN625296) Images
Purpose Human ErbB2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivity < 8 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details HER2 ELISA Kit Application Details Handling References for HER2 Kit (ABIN625296) Images back to top
Antigen
Alternative Name ErbB2 (ERBB2 ELISA Kit Abstract)
Background The Human ErbB2 (Epidermal Factor Growth Factor Receptor 2) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ErbB2 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human ErbB2 coated on a 96-well plate. Standards and samples are pipetted into the wells and ErbB2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ErbB2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ErbB2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details HER2 ELISA Kit Target details Handling References for HER2 Kit (ABIN625296) Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernantants and urine. Suggested dilution for normal serum/plasma: 2 fold*. *Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent B should be diluted 5-fold with deionized or distilled water before use.
4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture supernates/urine) into Item C vial to prepare a 50 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 20 µL ErbB2 standard (50 ng/mL) from the vial of Item C, into a tube with 480 µL Assay Diluent A or 1x Assay Diluent B to prepare a 2,000 pg/mL standard solution. Pipette 300 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the 2,000 pg/mL standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 20 µL standard + 480 µL 200 µL 200 µL 200 µL 200 µL 200 µL 200myl 2,000 800 320 12.0 51.2 20.48 8.19 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 300-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 40 µL of HRP-Streptavidin concentrate into a tube with 12 ml 1x Assay Diluent B to prepare a final 300 fold diluted HRP- Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent B Human ErbB2 concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.001 0.01 0.1 1 10 Assay Diluent A Human ErbB2 concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.001 0.01 0.1 1 10
Sensitivity: The minimum detectable dose of ErbB2 is typically less than 8 pg/mL.
Recovery: Recovery was determined by spiking various levels human ErbB2 into human serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 91.93 76-106 Plasma 81.32 69-97 Cell culture media 82.77 70-96
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 112.9 119.9 113.8 Range ( %) 102-121 112-127 104-122 1:4 Average % of Expected 120.4 119.7 125.5 Range ( %) 111-130 113-130 118-132
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only

Handling

Product Details HER2 ELISA Kit Target details Application Details References for HER2 Kit (ABIN625296) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

References for HER2 Kit (ABIN625296)

Product Details HER2 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Laidi, Bouziane, Lakhdar, Khabouze, Rhrab, Zaoui: "Salivary expression of soluble HER2 in breast cancer patients with positive and negative HER2 status." in: OncoTargets and therapy, Vol. 7, pp. 1285-9, 2014

Images

Product Details HER2 ELISA Kit Target details Application Details Handling References for HER2 Kit (ABIN625296) back to top
Supplier Images
ELISA image for HER2 ELISA Kit (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (ABIN625296) V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) ELISA Kit