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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||5 Antibodies|
|Flow Cytometry (FACS)||10 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||7 Antibodies|
|Immunodiffusion (ID)||1 Antibodies|
|Immunoelectrophoresis (IEP)||1 Antibodies|
|Immunofluorescence (IF)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||51 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||25 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Neutralization (Neut)||4 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Western Blotting (WB)||119 Antibodies|
|Antigen||Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) Antibodies|
|Conjugate||This HGF antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-HGF AntibodyTarget Details HGF Application Details Handling References for anti-HGF Antibody (ABIN357192) Images
|Specificity||This antibody detects HGF at C-term.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Human HGF.|
Target Details HGFProduct Details anti-HGF Antibody Application Details Handling References for anti-HGF Antibody (ABIN357192) Images back to top
|Alternative Name||Hepatocyte Growth Factor / HGF (HGF Antibody Abstract)|
|Background||Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69- kDa alpha-chain and 34- kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. Transcript Variant: This variant (1) encodes the longest isoform (1). To date, experimental evidence for cleavage of the proprotein into two mature chains has been shown only for isoform 1.Synonyms: HPTA, Hepatopoeitin-A, Scatter factor|
|Molecular Weight||83134 Da|
|Gene ID||3082, 9606|
Application DetailsProduct Details anti-HGF Antibody Target Details HGF Handling References for anti-HGF Antibody (ABIN357192) Images back to top
ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-HGF Antibody Target Details HGF Application Details References for anti-HGF Antibody (ABIN357192) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.|
References for anti-HGF Antibody (ABIN357192)Product Details anti-HGF Antibody Target Details HGF Application Details Handling Images back to top
Matsuda-Hashii, Takai, Ohta et al.: "Hepatocyte growth factor plays roles in the induction and autocrine maintenance of bone marrow stromal cell IL-11, SDF-1 alpha, and stem cell factor." in: Experimental hematology, Vol. 32, Issue 10, pp. 955-61, 2004 (PubMed).
He, Zhou, Dou et al.: "Autocrine expression of hepatocyte growth factor and its cytoprotective effect on hepatocyte poisoning." in: World journal of gastroenterology : WJG, Vol. 10, Issue 19, pp. 2827-30, 2004 (PubMed).
Lyon, Deakin, Lietha et al.: "The interactions of hepatocyte growth factor/scatter factor and its NK1 and NK2 variants with glycosaminoglycans using a modified gel mobility shift assay. Elucidation of the minimal size of binding ..." in: The Journal of biological chemistry, Vol. 279, Issue 42, pp. 43560-7, 2004 (PubMed).
Solntsev, Sieler, Krautscheid et al.: "Fused pyridazines: rigid multidentates for designing and fine-tuning the structure of hybrid organic/inorganic frameworks." in: Dalton transactions (Cambridge, England : 2003), Issue 8, pp. 1153-8, 2004 (PubMed).
Tjin, Derksen, Kataoka et al.: "Multiple myeloma cells catalyze hepatocyte growth factor (HGF) activation by secreting the serine protease HGF-activator." in: Blood, Vol. 104, Issue 7, pp. 2172-5, 2004 (PubMed).
ImagesProduct Details anti-HGF Antibody Target Details HGF Application Details Handling References for anti-HGF Antibody (ABIN357192) back to top