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anti-Human HGF antibody for Enzyme Immunoassay

Recommended HGF Antibody (supplied by: Log in to see )

Antigen
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) Antibodies
  • AI838057
  • c-Met
  • C230052L06Rik
  • DFNB39
  • F-TCF
  • HGF
  • HGF/SF
  • HGFB
  • HGFR
  • HPTA
  • NK1
  • NK2
  • Par4
  • SF
  • SF/HGF
Epitope
C-Term
24
17
16
14
13
9
8
7
6
5
5
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
210
44
39
9
2
2
2
1
Host
Rabbit
136
93
4
4
Clonality
Polyclonal
Conjugate
This HGF antibody is un-conjugated
18
14
6
5
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
175
113
61
29
11
11
10
8
6
5
4
2
2
1
1
1
1
1
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Catalog No. ABIN357192
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN783138 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008867729 ABIN115703 EIA WB Rabbit Log in to see Polyclonal
0.0008867729 ABIN115704 EIA WB Rabbit Log in to see Polyclonal
0.0008867729 ABIN115705 EIA WB Rabbit Log in to see Polyclonal

Top referenced anti-HGF antibody for Enzyme Immunoassay

  • Human Polyclonal HGF Primary Antibody for EIA, IHC (p) - ABIN357192 (5 Publications): Lyon, Deakin, Lietha, Gherardi, Gallagher et al.: The interactions of hepatocyte growth factor/scatter factor and its NK1 and NK2 variants with glycosaminoglycans using a modified gel mobility shift assay. Elucidation of the minimal size of binding ... in The Journal of biological chemistry 2004 (PubMed)

Similar anti-HGF Antibodies

Application / Reactivity Human
ELISA 98 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunodiffusion (ID) 1 Antibodies
Immunoelectrophoresis (IEP) 1 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 55 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Neutralization (Neut) 5 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) Antibodies
Epitope C-Term
(24), (17), (16), (14), (13), (9), (8), (7), (6), (5), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(210), (44), (39), (9), (2), (2), (2), (1)
Host Rabbit
(136), (93), (4), (4)
Clonality
Polyclonal
Conjugate This HGF antibody is un-conjugated
(18), (14), (6), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(175), (113), (61), (29), (11), (11), (10), (8), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-HGF Antibody

Target Details HGF Application Details Handling References for anti-HGF Antibody (ABIN357192) Images
Specificity This antibody detects HGF at C-term.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Human HGF.
Isotype Ig

Target Details HGF

Product Details anti-HGF Antibody Application Details Handling References for anti-HGF Antibody (ABIN357192) Images back to top
Antigen
Alternative Name Hepatocyte Growth Factor / HGF (HGF Antibody Abstract)
Background Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69- kDa alpha-chain and 34- kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. Transcript Variant: This variant (1) encodes the longest isoform (1). To date, experimental evidence for cleavage of the proprotein into two mature chains has been shown only for isoform 1.Synonyms: HPTA, Hepatopoeitin-A, Scatter factor
Molecular Weight 83134 Da
Gene ID 3082, 9606
UniProt P14210
Pathways RTK Signaling

Application Details

Product Details anti-HGF Antibody Target Details HGF Handling References for anti-HGF Antibody (ABIN357192) Images back to top
Application Notes ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HGF Antibody Target Details HGF Application Details References for anti-HGF Antibody (ABIN357192) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-HGF Antibody (ABIN357192)

Product Details anti-HGF Antibody Target Details HGF Application Details Handling Images back to top
Background publications

Lyon, Deakin, Lietha, Gherardi, Gallagher et al.: "The interactions of hepatocyte growth factor/scatter factor and its NK1 and NK2 variants with glycosaminoglycans using a modified gel mobility shift assay. Elucidation of the minimal size of binding ..." in: The Journal of biological chemistry, Vol. 279, Issue 42, pp. 43560-7, 2004

He, Zhou, Dou, Chen, Yan, Li: "Autocrine expression of hepatocyte growth factor and its cytoprotective effect on hepatocyte poisoning." in: World journal of gastroenterology : WJG, Vol. 10, Issue 19, pp. 2827-30, 2004

Tjin, Derksen, Kataoka, Spaargaren, Pals: "Multiple myeloma cells catalyze hepatocyte growth factor (HGF) activation by secreting the serine protease HGF-activator." in: Blood, Vol. 104, Issue 7, pp. 2172-5, 2004

Matsuda-Hashii, Takai, Ohta, Fujisaki, Tokimasa, Osugi, Ozono, Matsumoto, Nakamura, Hara: "Hepatocyte growth factor plays roles in the induction and autocrine maintenance of bone marrow stromal cell IL-11, SDF-1 alpha, and stem cell factor." in: Experimental hematology, Vol. 32, Issue 10, pp. 955-61, 2004

Solntsev, Sieler, Krautscheid, Domasevitch: "Fused pyridazines: rigid multidentates for designing and fine-tuning the structure of hybrid organic/inorganic frameworks." in: Dalton transactions (Cambridge, England : 2003), Issue 8, pp. 1153-8, 2004

Images

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Supplier Images
 image for anti-HGF antibody (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor)) (C-Term) (ABIN357192) anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (C-Term) antibody
 image for anti-HGF antibody (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor)) (C-Term) (ABIN357192) anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (C-Term) antibody (Image 2)