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|Antigen||Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) Antibodies|
|Epitope||AA 1-728 Alternatives|
|Conjugate||This HGF antibody is un-conjugated Alternatives|
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|1 reference available|
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Product Details anti-HGF AntibodyTarget Details HGF Application Details Handling References for anti-HGF antibody (ABIN1996875) Images
|Characteristics||Produced in rabbits immunized with purified, recombinant Human HGF (rh HGF, ABIN2215697 NP_000592.3, Met 1-Ser 728,. HGF specific IgG was purified by Human HGF affinity chromatography.|
|Purification||purified by affinity chromatography|
|Sterility||0.2 μm filtered|
|Immunogen||Recombinant Human HGF protein (ABIN2215697)|
Target Details HGFProduct Details anti-HGF Antibody Application Details Handling References for anti-HGF antibody (ABIN1996875) Images back to top
|Alternative Name||HGF (HGF Antibody Abstract)|
|Background||Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. Hepatocyte growth factor is secreted by mesenchymal cellsas a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.|
|Research Area||Cancer, Angiogenesis, Hormones, Growth Factors|
|Pathways||RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-HGF Antibody Target Details HGF Handling References for anti-HGF antibody (ABIN1996875) Images back to top
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human HGF. The detection limit for Human HGF is approximately 0.00245 ng/well.
IHC-P: 1-10 μg/mL
|Restrictions||For Research Use only|
HandlingProduct Details anti-HGF Antibody Target Details HGF Application Details References for anti-HGF antibody (ABIN1996875) Images back to top
|Buffer||0.2 μm filtered solution in PBS with 5 % trehalose|
|Handling Advice||Avoid repeated freeze-thaw cycles.|
|Storage||4 °C,-20 °C,-80 °C|
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
|Expiry Date||12 months|
References for anti-HGF antibody (ABIN1996875)Product Details anti-HGF Antibody Target Details HGF Application Details Handling Images back to top
Comoglio: "Structure, biosynthesis and biochemical properties of the HGF receptor in normal and malignant cells." in: EXS, Vol. 65, pp. 131-65, 1993
ImagesProduct Details anti-HGF Antibody Target Details HGF Application Details Handling References for anti-HGF antibody (ABIN1996875) back to top