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|Antigen||Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) Antibodies|
|Conjugate||This HGF antibody is un-conjugated Alternatives|
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Product Details anti-HGF AntibodyTarget Details HGF Application Details Handling Images
Human HGF / Hepatocyte Growth Factor
No cross-reactivity with Mouse HGF (Catalog # 50038-M08H) and Rat HGF (Catalog # 80429-RNAH) in ELISA assay
|No Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
|Characteristics||It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 Molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified, recombinant Human HGF / Hepatocyte Growth Factor. The antibody was produced using recombinant antibody technology.|
|Sterility||0.2 μm filtered|
|Immunogen||Recombinant Human HGF / Hepatocyte Growth Factor protein (ABIN2215697)|
Target Details HGFProduct Details anti-HGF Antibody Application Details Handling Images back to top
|Alternative Name||HGF (HGF Antibody Abstract)|
|Research Area||Cancer, Angiogenesis, Hormones, Growth Factors|
|Pathways||RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-HGF Antibody Target Details HGF Handling Images back to top
In a functional ELISA which immobilized Human HGF (Catalog # 10377-H03H) at 2 μg/mL (100 μL/well) in the plate, the Mouse and Human Chimeric anti-Human Monoclonal Antibody (Catalog # 10463-mh010) can block the binding of 4 μg/mL of recombinant Human HGFR (Catalog # 10084-H02H) to human HGF,the EC50 is 0.75 μg/mL.
The neutralization activity of HGF Neutralizing Antibody is Measured by its ability to neutralize autocrine HGF-induced proliferation in the U87 MG cell line. Proliferation of U87 MG cells elicited by autocrine HGF was inhibited by increasing concentrations of Human HGF Monoclonal Antibody. The IC50 is typically 1-4 μg/mL.
|Restrictions||For Research Use only|
HandlingProduct Details anti-HGF Antibody Target Details HGF Application Details Images back to top
|Buffer||0.2 μm filtered solution in PBS|
|Handling Advice||Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.|
|Storage||4 °C,-20 °C,-80 °C|
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
|Expiry Date||12 months|
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