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anti-Mouse (Murine) HGF antibody for Immunocytochemistry

Recommended HGF Antibody (supplied by: Log in to see )

Antigen
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) Antibodies
  • AI838057
  • c-Met
  • C230052L06Rik
  • DFNB39
  • F-TCF
  • HGF
  • HGF/SF
  • HGFB
  • HGFR
  • HPTA
  • NK1
  • NK2
  • Par4
  • SF
  • SF/HGF
Epitope
AA 482-710
25
16
16
14
13
9
8
7
5
5
5
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
166
42
39
9
2
2
2
1
Host
Rabbit
133
51
4
4
Clonality
Polyclonal
Conjugate
This HGF antibody is un-conjugated
16
12
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
131
74
56
27
11
10
10
7
5
5
4
2
2
1
1
1
1
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Catalog No. ABIN1859144
$ 356.40
Plus shipping costs $45.00

Similar anti-HGF Antibodies

Application / Reactivity Mouse (Murine)
ELISA 10 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 27 Antibodies

General

Antigen Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) Antibodies
Epitope AA 482-710
(25), (16), (16), (14), (13), (9), (8), (7), (5), (5), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(166), (42), (39), (9), (2), (2), (2), (1)
Host Rabbit
(133), (51), (4), (4)
Clonality
Polyclonal
Conjugate This HGF antibody is un-conjugated
(16), (12), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(131), (74), (56), (27), (11), (10), (10), (7), (5), (5), (4), (2), (2), (1), (1), (1), (1)
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Product Details anti-HGF Antibody

Target Details HGF Application Details Handling Images
Sequence MGHHHHHHSG SEF-DHPVISCAK TKQLRVVNGI PTQTTVGWMV SLKYRNKHIC GGSLIKESWV LTARQCFPAR NKDLKDYEAW LGIHDVHERG EEKRKQILNI SQLVYGPEGS DLVLLKLARP AILDNFVSTI DLPSYGCTIP EKTTCSIYGW GYTGLINADG LLRVAHLYIM GNEKCSQHHQ GKVTLNESEL CAGAEKIGSG PCEGDYGGPL ICEQHKMRMV LGVIVPGRGC AIPNRPGIFV
Specificity It has been selected for its ability to recognize HGF in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen HGF (AA 482-710)
Isotype IgG

Target Details HGF

Product Details anti-HGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name Hepatocyte Growth Factor (HGF) (HGF Antibody Abstract)
Pathways RTK Signaling

Application Details

Product Details anti-HGF Antibody Target Details HGF Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant HGF (Asp482~Val710) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-HGF Antibody Target Details HGF Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-HGF Antibody Target Details HGF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-HGF antibody (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor)) (AA 482-710) (ABIN1859144) anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 482-710) antibody