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Mouse (Murine) HGF ELISA Kit

Recommended HGF Product (supplied by: Log in to see )

Antigen
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) ELISA Kits
  • AI838057
  • c-Met
  • C230052L06Rik
  • DFNB39
  • F-TCF
  • HGF
  • HGF/SF
  • HGFB
  • HGFR
  • HPTA
  • NK1
  • NK2
  • Par4
  • SF
  • SF/HGF
Epitope
AA 33-495, AA 496-728
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
32
16
16
11
10
8
4
3
3
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN2859322
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN415597 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see 2
0.0012117105 ABIN365445 Sandwich ELISA Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see 1
0.0012117105 ABIN625126 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant Log in to see 13
0.0012117105 ABIN1115407 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.16 ng/mL Log in to see
0.0012117105 ABIN625127 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see
0.0012117105 ABIN2684466 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.614-60 ng/mL 0.614 ng/mL Log in to see 1
0.0012117105 ABIN1570289 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012117105 ABIN512719 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012117105 ABIN2684467 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
0.0012117105 ABIN2534432 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012117105 ABIN455340 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012117105 ABIN2086491 Sandwich ELISA Log in to see
0.0012117105 ABIN2948251 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012117105 ABIN2116009 Log in to see

Top referenced HGF ELISA Kit for ELISA

Similar HGF ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 15 ELISA Kits

General

Antigen Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) ELISA Kits
Epitope AA 33-495, AA 496-728
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(32), (16), (16), (11), (10), (8), (4), (3), (3), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details HGF ELISA Kit

Target details Application Details Handling References for HGF Kit (ABIN2859322) Images
Purpose For quantitative detection of mouse HGF in cell culture supernates, serum and plasma(EDTA, citrate).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate)
Detection Method Colorimetric
Specificity Natural and recombinant mouse HGF
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse HGF antibod - 1
  • Lyophilized recombinant mouse HGF standar - 10ng/tubex2
  • Biotinylated anti- mouse HGF antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Immunogen Expression system for standard: NSO
Immunogen sequence: Q33-R495 (alpha)&V496-L728 (beta)

Target details

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Antigen
Alternative Name HGF (HGF ELISA Kit Abstract)
Background Hepatocyte growth factor(HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass(Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.
Gene ID 15234
UniProt Q08048
Research Area Cancer, Angiogenesis, Hormones, Growth Factors
Pathways RTK Signaling

Application Details

Product Details HGF ELISA Kit Target details Handling References for HGF Kit (ABIN2859322) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse HGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for HGF has been precoated onto 96-well plates. Standards(NSO,Q33-R495 (α) & V496 - L728 (β)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse HGF amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse HGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse HGF standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 427, Standard deviation: 17.93, CV(%): 4.2
  • Sample 2: n=16, Mean(pg/ml): 1523, Standard deviation: 76.15, CV(%): 5.0
  • Sample 3: n=16, Mean(pg/ml): 3342, Standard deviation: 187.15, CV(%): 5.6,
  • Sample 1: n=24, Mean(pg/ml): 501, Standard deviation: 37.58, CV(%): 7.5
  • Sample 2: n=24, Mean(pg/ml): 1756, Standard deviation: 136.97, CV(%): 7.8
  • Sample 3: n=24, Mean(pg/ml): 3471, Standard deviation: 284.62, CV(%): 8.2
Restrictions For Research Use only

Handling

Product Details HGF ELISA Kit Target details Application Details References for HGF Kit (ABIN2859322) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for HGF Kit (ABIN2859322)

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Product cited in:

Secchiero, Corallini, Zavan, Tripodo, Vindigni, Zauli: "Mesenchymal stem cells display hepato-protective activity in lymphoma bearing xenografts." in: Investigational new drugs, Vol. 30, Issue 2, pp. 803-7, 2012

Images

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Supplier Images
ELISA image for HGF ELISA Kit (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor)) (ABIN2859322) Mouse HGF PicoKine ELISA Kit standard curve