We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Pig (Porcine)|
|ELISA||4 ELISA Kits|
|Antigen||Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) ELISA Kits|
|Reactivity||Pig (Porcine) Alternatives|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||62.5-4000 pg/mL|
|Minimum Detection Limit||62.5 pg/mL|
|2 references available|
|Supplier||Log in to see|
Product Details HGF ELISA KitTarget details Application Details Handling References for HGF Kit (ABIN367412) Images
|Purpose||For the quantitative determination of pig hepatocyte growth factor (HGF) concentrations in serum, plasma.|
|Sample Type||Serum, Plasma, Tissue Homogenate|
|Specificity||This assay has high sensitivity and excellent specificity for detection of pig HGF.|
|Cross-Reactivity (Details)||Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.|
|Material not included||
Target detailsProduct Details HGF ELISA Kit Application Details Handling References for HGF Kit (ABIN367412) Images back to top
|Alternative Name||Hepatocyte growth factor (HGF) (HGF ELISA Kit Abstract)|
Application DetailsProduct Details HGF ELISA Kit Target details Handling References for HGF Kit (ABIN367412) Images back to top
Detection wavelength: 450 nm
|Sample Volume||50 μL|
|Assay Time||1 - 4.5 h|
|Plate||Pre-coated,Strips (12 x 8)|
|Protocol||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HGF bound in the initial step. The color development is stopped and the intensity of the color is measured.|
Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
|Restrictions||For Research Use only|
HandlingProduct Details HGF ELISA Kit Target details Application Details References for HGF Kit (ABIN367412) Images back to top
|Precaution of Use||The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.|
|Storage||4 °C/-20 °C|
|Storage Comment||May be stored at 2-8°C for up to 1 month. For long term storage, please store at -20°C. Try to keep assay plate in a sealed aluminium foil bag and avoid dampness.|
|Expiry Date||6 months|
References for HGF Kit (ABIN367412)Product Details HGF ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Zhou, Li, Zhang et al.: "Efficacy of coupled low-volume plasma exchange with plasma filtration adsorption in treating pigs with acute liver failure: A randomised study." in: Journal of hepatology, Vol. 63, Issue 2, pp. 378-87, 2015 (PubMed).
Shahid, Iwamuro, Sasamoto et al.: "Establishment of an immortalized porcine liver cell line JSNK-1 with retroviral transduction of SV40T." in: Cell transplantation, Vol. 19, Issue 6, pp. 849-56, 2010 (PubMed).