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anti-Human IGF1R antibody for Western Blotting

Recommended IGF1R Antibody (supplied by: Log in to see )

Antigen
Insulin-Like Growth Factor 1 Receptor (IGF1R) Antibodies
  • A330103N21Rik
  • CD221
  • D930020L01
  • DKFZp469I0618
  • hyft
  • IGF-1R
  • igf-1r
  • IGF1R
  • IGFIR
  • IGFIRC
  • IGFR
  • IGFR1
  • JTK13
  • xIGF-1R
  • xigf1r
  • xIGFR
Epitope
AA 260-290
31
30
28
26
24
21
19
14
13
13
13
13
13
13
12
8
7
6
5
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
429
171
169
2
2
2
2
1
1
Host
Rabbit
295
129
2
2
Clonality
Polyclonal
Conjugate
This IGF1R antibody is un-conjugated
20
19
18
16
10
9
8
8
8
8
8
8
8
8
6
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
263
107
100
87
70
58
38
29
9
8
7
4
3
3
2
1
1
1
1
Supplier
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Available images

Catalog No. ABIN726575
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN359877 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 5
0.13341247 ABIN391990 IHC (p) WB Rabbit Ig AA 19-51, N-Term Log in to see Polyclonal 5
0.13341247 ABIN652374 FACS IF IHC (p) WB Rabbit Ig AA 51-77, N-Term Log in to see Polyclonal 3
0.13341247 ABIN2792087 IHC WB Rabbit Middle Region Log in to see Polyclonal 2
0.13341247 ABIN616037 WB Rabbit pTyr1280 Log in to see Polyclonal 2
0.13341247 ABIN969207 IHC ELISA WB Mouse IgG2a AA 1101-1367 Log in to see 3G5C1 2
0.13341247 ABIN126815 WB Mouse IgG1 C-Term Log in to see 7G11
0.13341247 ABIN126813 EIA WB Mouse IgG1 pTyr1316 Log in to see 2B9
0.13341247 ABIN1531870 IHC ELISA WB Rabbit IgG pTyr1161 Log in to see Polyclonal
0.13341247 ABIN1531871 ELISA WB Rabbit IgG pTyr1165, pTyr1166 Log in to see Polyclonal
0.13341247 ABIN1533056 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN1532901 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN1532902 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN3044138 IHC (p) WB Rabbit IgG AA 1353-1367, C-Term Log in to see Polyclonal
0.13341247 ABIN516939 PLA WB Rabbit AA 1-1367, full length Log in to see Polyclonal 1
0.13341247 ABIN1106090 IHC (p) WB Mouse IgG2b Log in to see 3C8B1 3
0.13341247 ABIN1870257 IF IHC WB Rabbit IgG pTyr1161 Log in to see Polyclonal
0.13341247 ABIN1870256 IF WB Rabbit IgG pTyr1165, pTyr1166 Log in to see Polyclonal
0.13341247 ABIN2464067 IHC ELISA WB Mouse IgG2b truncated Log in to see 3C8B1
0.13341247 ABIN2464068 ELISA WB Mouse IgG2b truncated Log in to see 3G5C1

Top referenced anti-IGF1R antibody for Western Blotting

Similar anti-IGF1R Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 19 Antibodies 19 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 2 Antibodies
Immunofluorescence (IF) 6 Antibodies 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 88 Antibodies 88 Antibodies
Immunohistochemistry (IHC) 29 Antibodies 30 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Western Blotting (WB) 74 Antibodies

General

Antigen Insulin-Like Growth Factor 1 Receptor (IGF1R) Antibodies
Epitope AA 260-290
(31), (30), (28), (26), (24), (21), (19), (14), (13), (13), (13), (13), (13), (13), (12), (8), (7), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(429), (171), (169), (2), (2), (2), (2), (1), (1)
Host Rabbit
(295), (129), (2), (2)
Clonality
Polyclonal
Conjugate This IGF1R antibody is un-conjugated
(20), (19), (18), (16), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (6)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(263), (107), (100), (87), (70), (58), (38), (29), (9), (8), (7), (4), (3), (3), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IGF1R Antibody

Target Details IGF1R Application Details Handling References for anti-IGF1R Antibody (ABIN726575) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human IGF-1R alpha chain
Isotype IgG

Target Details IGF1R

Product Details anti-IGF1R Antibody Application Details Handling References for anti-IGF1R Antibody (ABIN726575) Images back to top
Antigen
Alternative Name IGF1R (IGF1R Antibody Abstract)
Background

When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.

Subcellular location: Extracellular


Synonyms: IGFR, CD221, IGFIR, JTK13, Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, IGF1R
Gene ID 3480
UniProt P08069
Pathways RTK Signaling

Application Details

Product Details anti-IGF1R Antibody Target Details IGF1R Handling References for anti-IGF1R Antibody (ABIN726575) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-IGF1R Antibody Target Details IGF1R Application Details References for anti-IGF1R Antibody (ABIN726575) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-IGF1R Antibody (ABIN726575)

Product Details anti-IGF1R Antibody Target Details IGF1R Application Details Handling Images back to top
Product cited in:

Hu, Li, Hu, Wu, Li, Meng: "MicroRNA let-7a and let-7f as novel regulatory factors of the sika deer (Cervus nippon) IGF-1R gene." in: Growth factors (Chur, Switzerland), Vol. 32, Issue 1, pp. 27-33, 2014

Yang, Liu, Li, Wen, Zhu, Xu: "Insulin-Like Growth Factor-1 Modulates Polycomb Cbx8 Expression and Inhibits Colon Cancer Cell Apoptosis." in: Cell biochemistry and biophysics, 2014

Images

Product Details anti-IGF1R Antibody Target Details IGF1R Application Details Handling References for anti-IGF1R Antibody (ABIN726575) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-IGF1R antibody (Insulin-Like Growth Factor 1 Receptor) (AA 260-290) (ABIN726575) Formalin-fixed and paraffin-embedded human lung carcinoma tissue labeled with Rabbit ...
Immunohistochemistry (IHC) image for anti-IGF1R antibody (Insulin-Like Growth Factor 1 Receptor) (AA 260-290) (ABIN726575) Formalin-fixed and paraffin embedded: human lung carcinoma labeled with Anti-IGF1R/CD...
Flow Cytometry (FACS) image for anti-IGF1R antibody (Insulin-Like Growth Factor 1 Receptor) (AA 260-290) (ABIN726575) Mouse splenocytes probed with Rabbit Anti-IGF1R Polyclonal Antibody .
'Independent Validation' Badge
Lot Number 130322
Method validated Western Blotting
Positive Control MCF-7 cells – high expression
Negative Control PC3 cells – low expression
Notes Two strong bands were observed in the positive control, one at the expected molecular weight, and one slightly higher (which may represent a glycosylated or precursor form). Weaker bands of the same molecular weight appear in the negative control, which is consistent with the expected lower expression in PC3 cells. No other major bands are present in either positive or negative controls.
Primary Antibody
  • Antigen: Insulin-Like Growth Factor 1 Receptor (IGF1R) (1:200 dilution)
  • Catalog number: ABIN726575
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 130322
Secondary Antibody
  • Antibody: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: L170-6515
Controls
  • Positive control: MCF-7 cell extract
  • Negative control: PC-3 cell extract
Protocol
  • Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
  • 46 μg of boiled extracts were loaded and resolved on a 8-16% SDS-polyacrylamide gel.
  • The Spectra Multicolor Broad Range (Thermo Scientific, Cat # 26634) were used as molecular mass markers.
  • Proteins were transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) non-fat dry milk at room temperature for 1 h.
  • The membrane was rinsed with TBST once.
  • The membrane was immersed with the protein side up in the primary antibody solution (anti-IGF1R; 1:200) in TBST containing 5% (W/V) non-fat dry milk and incubated for 16 hours at 4°C.
  • The membrane was rinsed in TBST three times for 5 min each.
  • The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) non-fat dry milk and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • The membrane was rinsed three times with TBST for 5 min each.
  • The membrane was rinsed in TBS twice for 30 s each.
  • Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
  • The membrane was rinsed three times with TBST, then incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
  • The membrane was washed in TBST 2 times for 10 min each.
  • Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
Experimental Notes - No challenges noted.
Validation Images
Western Blotting validation image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 260-290) antibody (ABIN726575) Figure 1. Western blot of lysates from MCF7 cells (Lane 1) and PC-3 cells (Lane 2) pr...