anti-Mouse (Murine) IL34 antibody for ELISA

Recommended IL34 Antibody (supplied by: Log in to see )

Antigen
Interleukin 34 (IL34) Antibodies
  • C16orf77
  • IL-34
  • 2010004A03Rik
  • AI593503
  • C18H16ORF77
  • interleukin 34
  • interleukin-34-like
  • IL34
  • Il34
  • LOC100347811
  • LOC100737468
Epitope
N-Term
28
18
8
8
8
5
3
3
2
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
113
31
21
1
1
Host
Rabbit
62
60
7
3
1
Clonality
Polyclonal
Conjugate
This IL34 antibody is un-conjugated
15
5
5
4
4
4
4
3
3
3
3
3
2
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
75
74
44
11
11
10
4
4
3
2
2
2
2
2
1
1
Supplier
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Catalog No. ABIN1002640
$ 466.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1002641 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 1
1 ABIN4327331 ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN4327330 ELISA WB Rabbit Center Log in to see Polyclonal
1 ABIN2609915 ELISA WB Biotin Rabbit IgG AA 21-235 Log in to see Polyclonal
1 ABIN2609916 ELISA WB FITC Rabbit IgG AA 21-235 Log in to see Polyclonal
1 ABIN603379 ELISA WB Sheep IgG Log in to see Polyclonal
1 ABIN1030960 ELISA WB Rabbit Middle Region Log in to see Polyclonal
1 ABIN1031416 ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2609918 ELISA WB Mouse IgG AA 21-235 Log in to see
1 ABIN2609912 ELISA WB Rabbit IgG AA 21-235 Log in to see Polyclonal
1 ABIN1175350 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN1887964 ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1887482 ELISA WB Rabbit Center Log in to see Polyclonal
1 ABIN2289073 ELISA WB Sheep IgG Log in to see Polyclonal
1 ABIN1584603 ELISA WB Rabbit Log in to see Polyclonal
1 ABIN1584602 ELISA WB Rabbit Log in to see Polyclonal
1 ABIN1980444 ICC IHC (fro) IHC (p) ELISA WB Mouse AA 21-235 Log in to see
1 ABIN2935509 ELISA IF/ICC IHC WB Rabbit AA 21-235 Log in to see Polyclonal

General

Antigen Interleukin 34 (IL34) Antibodies
Epitope N-Term
(28), (18), (8), (8), (8), (5), (3), (3), (2), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(113), (31), (21), (1), (1)
Host Rabbit
(62), (60), (7), (3), (1)
Clonality
Polyclonal
Conjugate This IL34 antibody is un-conjugated
(15), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(75), (74), (44), (11), (11), (10), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL34 Antibody

Target Details IL34 Application Details Handling References for anti-IL34 antibody (ABIN1002640) Images
Blocking Peptide Blocking peptide for this product available: ABIN1004152
Purification Affinity chromatography purified via peptide column.
Immunogen IL-34 antibody was raised in rabbits against a 16 amino acid peptide from near the amino terminus of human IL-34.
Isotype IgG

Target Details IL34

Product Details anti-IL34 Antibody Application Details Handling References for anti-IL34 antibody (ABIN1002640) Images back to top
Antigen
Alternative Name Interleukin 34 (IL34 Antibody Abstract)
Background Interleukin-34 (IL-34) was identified through a functional screening of a library of secreted proteins after transfection of an embryonic kidney cell line with recombinant cDNAs. Flow cytometry and monocyte viability assays demonstrated increased survival and/or proliferation of monocytes induced by IL-34 and specific binding of IL-34 to CD14+ monocytes. IL-34 is a second ligand to the colony stimulating factor (CSF)-1 receptor in addition to CSF1. Like CSF1, IL-34 stimulates phosphorylation of ERK1 and ERK2 in human monocytes and induces differentiation of bone marrow cells into macrophages. Despite its predicted molecular weight, IL-34 will often run at higher molecular weight in SDS-PAGE.
Research Area Immunology, Innate Immunity, Cytokines
Pathways RTK Signaling

Application Details

Product Details anti-IL34 Antibody Target Details IL34 Handling References for anti-IL34 antibody (ABIN1002640) Images back to top
Application Notes IL-34 antibody can be used for the detection of IL-34 by Western blot at 0.25 – 0.5 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only

Handling

Product Details anti-IL34 Antibody Target Details IL34 Application Details References for anti-IL34 antibody (ABIN1002640) Images back to top
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months

References for anti-IL34 antibody (ABIN1002640)

Product Details anti-IL34 Antibody Target Details IL34 Application Details Handling Images back to top
Product cited in:

Lin, Lee, Hestir, Leo, Huang, Bosch, Halenbeck, Wu, Zhou, Behrens, Hollenbaugh, Linnemann, Qin, Wong, Chu, Doberstein, Williams: "Discovery of a cytokine and its receptor by functional screening of the extracellular proteome." in: Science (New York, N.Y.), Vol. 320, Issue 5877, pp. 807-11, 2008