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anti-Mouse (Murine) KIT Ligand antibody for Flow Cytometry

Recommended KIT Ligand Antibody (supplied by: Log in to see )

Antigen
KIT Ligand (KITLG) Antibodies
  • 710-712
  • blz
  • Clo
  • Con
  • contrasted
  • CSF
  • FPH2
  • Gb
  • Kitl
  • kitl
  • KITL
  • Kitlg
  • KITLG
  • KL-1
  • kl-1
  • Mgf
  • MGF
  • mgf
  • scf
  • SCF
  • SF
  • SHEP7
  • Sl
  • SLF
  • steel
  • Xkl-1
  • Xsl
  • Xsl-1
  • Xsl-2
Epitope
N-Term
21
14
9
8
7
6
5
4
4
3
3
3
3
3
3
3
2
2
2
1
1
1
1
Reactivity
Rat (Rattus), Mouse (Murine), Human
182
67
58
4
1
1
1
1
1
Host
Rabbit
188
45
22
1
Clonality
Polyclonal
Conjugate
This KIT Ligand antibody is un-conjugated
36
11
8
8
7
7
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
211
151
37
34
26
21
19
16
11
10
9
9
6
4
1
1
1
1
1
Supplier
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Catalog No. ABIN2017605
$ 257.40
Plus shipping costs $45.00

Similar anti-KIT Ligand Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 149 Antibodies 49 Antibodies 46 Antibodies
Radioimmunoassay (RIA) 2 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Neutralization (Neut) 10 Antibodies 9 Antibodies 15 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies 8 Antibodies 8 Antibodies
Immunohistochemistry (IHC) 36 Antibodies 9 Antibodies 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 4 Antibodies 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies

General

Antigen KIT Ligand (KITLG) Antibodies
Epitope N-Term
(21), (14), (9), (8), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Rat (Rattus), Mouse (Murine), Human
(182), (67), (58), (4), (1), (1), (1), (1), (1)
Host Rabbit
(188), (45), (22), (1)
Clonality
Polyclonal
Conjugate This KIT Ligand antibody is un-conjugated
(36), (11), (8), (8), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Western Blotting (WB)
(211), (151), (37), (34), (26), (21), (19), (16), (11), (10), (9), (9), (6), (4), (1), (1), (1), (1), (1)
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Product Details anti-KIT Ligand Antibody

Target Details KIT Ligand Application Details Handling Images
Specificity SCF Antibody detects endogenous levels of human and mouse SCF. It is predicted to react with rat SCF protein according to sequence homology.Positive Control: Ramos cell, mouse kidney tissue lysate
Cross-Reactivity (Details) SCF Antibody detects endogenous levels of human and mouse SCF. It is predicted to react with rat SCF protein according to sequence homology.Positive Control: Ramos cell, mouse kidney tissue lysate
Purification Immunoaffinity chromatography
Immunogen KLH-coupled synthetic peptide from N-terminal of human SCF
Isotype IgG2b

Target Details KIT Ligand

Product Details anti-KIT Ligand Antibody Application Details Handling Images back to top
Antigen
Alternative Name SCF (KITLG Antibody Abstract)
Background Stem cell factor (SCF) is a growth factor that is essential for hematopoiesis, melanogenesis, and fertility. It exerts its activity at the early stages of hematopoiesis. Specifically, SCF stimulates the proliferation of mast cell and mediates cell to cell adhesion and acts synergistically with other cytokines, probably interleukins. In addition, SCF induces dimerization of c-Kit followed by trans-autophosphorylation of the cytoplasmic protein tyrosine kinase domain, leading to subsequent recruitment of signaling proteins, tyrosine phosphorylation of substrates, and activation of multiple signaling pathways.
SCF Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human SCF (Swiss Prot: P21583).
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-KIT Ligand Antibody Target Details KIT Ligand Handling Images back to top
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther applications: user optimized
Restrictions For Research Use only

Handling

Product Details anti-KIT Ligand Antibody Target Details KIT Ligand Application Details Images back to top
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Images

Product Details anti-KIT Ligand Antibody Target Details KIT Ligand Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-KIT Ligand antibody (KITLG) (N-Term) (ABIN2017605) Western blot analysis of tissue lysates using SCF Antibody (ABIN399098, 1 µg/mL) The ...
Flow Cytometry (FACS) image for anti-KIT Ligand antibody (KITLG) (N-Term) (ABIN2017605) Flow cytometric analysis of Ramos cells using SCF Antibody (ABIN399098, shaded histog...