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|Antigen||KIT Ligand (KITLG) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This KIT Ligand antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
|1 reference available|
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Product Details anti-KIT Ligand AntibodyTarget Details KIT Ligand Application Details Handling References for anti-KIT Ligand antibody (ABIN115909) Images
|Specificity||This antibody recognises Murine Stem Cell Factor.|
|Immunogen||Highly pure (>98%) recombinant mSCF.|
Target Details KIT LigandProduct Details anti-KIT Ligand Antibody Application Details Handling References for anti-KIT Ligand antibody (ABIN115909) Images back to top
|Alternative Name||KITLG / SCF (KITLG Antibody Abstract)|
|Background||Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-KIT Ligand Antibody Target Details KIT Ligand Handling References for anti-KIT Ligand antibody (ABIN115909) Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (25.0 ng/mL),a concentration of 0.60-0.65 μg/mL of this antibody is required. ELISA: To detect mSCF by direct ELISA (using 100 l/well antibody solution) a concentration of atleast 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant mSCF. Western Blot: To detect mSCF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-KIT Ligand Antibody Target Details KIT Ligand Application Details References for anti-KIT Ligand antibody (ABIN115909) Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.|
References for anti-KIT Ligand antibody (ABIN115909)Product Details anti-KIT Ligand Antibody Target Details KIT Ligand Application Details Handling Images back to top
Chandra, Kapur, Chuzhanova, Summey, Prentice, Barker, Cooper, Williams: "A rare complex DNA rearrangement in the murine Steel gene results in exon duplication and a lethal phenotype." in: Blood, Vol. 102, Issue 10, pp. 3548-55, 2003