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|Antigen||C-Mer Proto-Oncogene Tyrosine Kinase (MERTK) ELISA Kits|
|Reactivity||Mouse (Murine) Alternatives|
Kits with alternative reactivity to:
|Method Type||Sandwich ELISA|
|Detection Range||78-5000 pg/mL|
|Minimum Detection Limit||78 pg/mL|
|1 reference available|
|Supplier||Log in to see|
Product Details MERTK ELISA KitTarget details Application Details Handling ProductDetails: References for MERTK Kit (ABIN1672806) Images
|Purpose||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MER/MERTK|
|Sample Type||Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum|
Expression system for standard: sf21
Immunogen sequence: E23-F498
|Cross-Reactivity (Details)||There is no detectable cross-reactivity with other relevant proteins.|
|Material not included||Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl|
Expression system for standard: sf21
Immunogen sequence: E23-F498
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|Alternative Name||MERTK (MERTK ELISA Kit Abstract)|
Protein Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll- like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. .
Background: Proto-oncogene tyrosine-protein kinase MER, also called MERTK is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type(immunoglobulin-like) domains, and one tyrosine kinase domain. This gene is mapped to 2q13. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium(RPE) as a regulator of rod outer segments fragments phagocytosis. This gene also plays an important role in inhibition of Toll-like receptors(TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
Synonyms: Tyrosine-protein kinase Mer,22.214.171.124,Proto-oncogene c-Mer,Receptor tyrosine kinase MerTK,Mertk,Mer,
Full Gene Name: Tyrosine-protein kinase MerCellular Localisation: Membrane, Single-pass type I membrane protein.
|Research Area||Neurology, Tyrosine Kinases|
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|Application Notes||Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.|
Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Tissue Specificity: Expressed predominantly in the hematopoietic lineages: macrophages, NK cells, NKT cells, dendritic cells and platelets. .
|Protocol||mouse MER ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MER has been precoated onto 96-well plates. Standards(sf21, E23-F498) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MER is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MER amount of sample captured in plate.|
|Assay Procedure||Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse MER standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, cell lysates, tissue homogenates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse MER standard solution and each sample is measured in duplicate.|
|Restrictions||For Research Use only|
HandlingProduct Details MERTK ELISA Kit Target details Application Details ProductDetails: References for MERTK Kit (ABIN1672806) Images back to top
|Handling Advice||Avoid multiple freeze-thaw cycles.|
|Storage||-20 °C,4 °C|
|Storage Comment||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles|
|Expiry Date||12 months|
ProductDetails: References for MERTK Kit (ABIN1672806)Product Details MERTK ELISA Kit Target details Application Details Handling Images back to top
Ebermann, Walger, Scholl, Charbel Issa, Lüke, Nürnberg, Lang-Roth, Becker, Nürnberg, Bolz: "Truncating mutation of the DFNB59 gene causes cochlear hearing impairment and central vestibular dysfunction." in: Human mutation, Vol. 28, Issue 6, pp. 571-7, 2007
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