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anti-Rat (Rattus) MME antibody for Enzyme Immunoassay

Recommended MME Antibody (supplied by: Log in to see )

Antigen
Membrane Metallo-Endopeptidase (MME) Antibodies
  • 6030454K05Rik
  • AV378681
  • C85356
  • CAK
  • CALLA
  • CD10
  • CD167
  • DDR
  • EDDR1
  • HGK2
  • MCK10
  • MME
  • Mmel
  • Nep
  • NEP
  • NTRK4
  • PTK3
  • PTK3A
  • RTK6
  • SFE
  • TRKE
Epitope
AA 123-145
34
21
19
16
14
12
7
6
6
6
6
6
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
568
64
62
15
15
1
Host
Rabbit
406
177
3
Clonality
Polyclonal
Conjugate
This MME antibody is un-conjugated
61
49
35
24
9
7
7
7
7
5
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
294
256
134
132
100
75
67
52
46
14
11
8
4
4
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN493163
$ 456.50
Plus shipping costs $45.00

Top referenced anti-MME antibody for Enzyme Immunoassay

Similar anti-MME Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine)
Western Blotting (WB) 244 Antibodies 42 Antibodies 47 Antibodies
Multiplex Assay (MA) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Immunoprecipitation (IP) 69 Antibodies 16 Antibodies 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 100 Antibodies 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 130 Antibodies 28 Antibodies 26 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies 3 Antibodies 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies

General

Antigen Membrane Metallo-Endopeptidase (MME) Antibodies
Epitope AA 123-145
(34), (21), (19), (16), (14), (12), (7), (6), (6), (6), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(568), (64), (62), (15), (15), (1)
Host Rabbit
(406), (177), (3)
Clonality
Polyclonal
Conjugate This MME antibody is un-conjugated
(61), (49), (35), (24), (9), (7), (7), (7), (7), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(294), (256), (134), (132), (100), (75), (67), (52), (46), (14), (11), (8), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-MME Antibody

Target Details MME Application Details Handling References for anti-MME Antibody (ABIN493163) Images
Sequence QEPKTEDIVA VQKAKALYRS CIN
Specificity This antibody detects CD10 / Neprilysin at aa 123-145). It does not cross react to other proteases.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Affinity chromatography
Immunogen Synthetic peptides corresponding to aa 123-145 of Neprilysin

Target Details MME

Product Details anti-MME Antibody Application Details Handling References for anti-MME Antibody (ABIN493163) Images back to top
Antigen
Alternative Name CD10 / Neprilysin (MME Antibody Abstract)
Background The mammalian neprilysin (NEP) family members are typically type II membrane endopeptidases responsible for the activation/inactivation of neuropeptides and peptide hormones. The substrate specificity and subcellualar localization of the 7 mammalian NEPs contribute to their functional heterogeneity. Proteolytic enzymes constitute around 2 % of the human genome and are several stages of cell development from fertilization to cell death as a result of apoptosis. The identification of many novel proteases from genome-sequencing programs has suggested them as potential new therapeutic targets. In addition, several well-characterized metallopeptidases were recently shown to possess new biological roles in neuroinflammation and neurodegeneration. Neprilysin, a zinc-metalloendopeptidase, located on chromosome 3q21-q27 contains 24 exons and plays important role in the physiology and pathology of many diseases such as hypertension, cancer and Alzheimer's disease (AD). In AD the deposition of amyloid beta peptides (Abeta) in brain form plaques that are hallmark of AD codition. Neprilysin is the major Abeta degrading enzyme and a reduction in Neprilysin activity contribute to the extent of plaque formation in AD by increasing the steady state levels of Abeta (1). The levels of Abeta in the central nervous system are regulated by several catabolic proteases, including insulysin (IDE), BACE1, BACE2, presenilin1, presenilin2, gamma secretase and neprilysin (NEP). Gleevec, a known tyrosine kinase inhibitor, has been shown to lower Abeta secretion, and it is considered a potential basis for novel therapies for Alzheimer's disease. In vitro treatment of cells with Gleevec elevates neprilysin levels that caused an increased Abeta degradation (2). Neutral endopeptiddase, Neprilysin is a major enzyme involved in the degradation and metablic inactivation of a number of biological peptides including enkephlins, substance P, bradykinin, incretin hormone glucagon like peptide 1 and arterial natriuretic peptides. Neprilysin hydrolyze the brain derived natriuretic peptide (BNP) but not the structurally related arterial or and C-type natriuretic peptides which are important cardioprotective hormones with essential function in sodium excretion, water balance, and blood pressure regulation. The Neprilysin is a 750 amino acid protein with predicted molecular weight of 86 kDa but as a result of abundant post translational modifications, specially glycosylation, the brain Neprilysin migrates between 98-110 kDa on denaturing SDS-PAGE. The Neprilysin is a membrane bound glycoprotein with a short N-termainl cytoplasmic tail, and TMD and a large C-terminal extracellular catalytic domain comprise of a zinc binding motif that also facilitates the small hydrophobic peptides. The Neprilysin is ubiquitously expressed in many tissues including intestinal BBM, kidney epithelial cells, neutrophils, thymocytes, lung, testis and in neuronal plasma brain (3).Synonyms: Atriopeptidase, CALLA, Common acute lymphocytic leukemia antigen, EPN, Enkephalinase, MME, NEP, Neutral endopeptidase, Neutral endopeptidase 24.11
Gene ID 4311
NCBI Accession NP_000893
UniProt P08473
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways RTK Signaling

Application Details

Product Details anti-MME Antibody Target Details MME Handling References for anti-MME Antibody (ABIN493163) Images back to top
Application Notes ELISA: < 1: 10,000. Western blot: > 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MME Antibody Target Details MME Application Details References for anti-MME Antibody (ABIN493163) Images back to top
Concentration 0.72 - 0.78 mg/mL
Buffer Stabilization buffer
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.

References for anti-MME Antibody (ABIN493163)

Product Details anti-MME Antibody Target Details MME Application Details Handling Images back to top
Background publications

Eisele, Baumann, Klebl, Nordhammer, Jucker, Kilger: "Gleevec increases levels of the amyloid precursor protein intracellular domain and of the amyloid-beta degrading enzyme neprilysin." in: Molecular biology of the cell, Vol. 18, Issue 9, pp. 3591-600, 2007

Wood, Pickering, McHale, Dechairo: "Association between neprilysin polymorphisms and sporadic Alzheimer's disease." in: Neuroscience letters, Vol. 427, Issue 2, pp. 103-6, 2007