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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||7 Antibodies||2 Antibodies||2 Antibodies|
|Cytometry by Time of Flight (CyTOF)||3 Antibodies|
|Western Blotting (WB)||251 Antibodies||56 Antibodies||69 Antibodies|
|Multiplex Assay (MA)||1 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Immunoprecipitation (IP)||71 Antibodies||20 Antibodies||19 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||108 Antibodies||13 Antibodies||16 Antibodies|
|Immunohistochemistry (IHC)||168 Antibodies||36 Antibodies||40 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||46 Antibodies||1 Antibodies|
|Antigen||Membrane Metallo-Endopeptidase (MME) Antibodies|
|Epitope||AA 52-750 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MME antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-MME AntibodyTarget Details MME Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Neprilysin(MME) detection. Tested with WB, IHC-P in Human,Mouse,Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Neprilysin(MME) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: membrane metallo-endopeptidase
Protein Name: Neprilysin
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human CD10 recombinant protein (Position: Y52-W750). Human CD10 shares 94% amino acid (aa) sequences identity with both mouse and rat CD10.|
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|Alternative Name||MME (MME Antibody Abstract)|
CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.
Synonyms: Atriopeptidase antibody|CALLA antibody|CD10 antibody|CD10 antigen antibody|Common acute lymphocytic leukemia antigen antibody|DKFZp686O16152 antibody|EC 220.127.116.11 antibody|Enkephalinase antibody|EPN antibody|gp100 antibody|Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody|Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody|Membrane metallo endopeptidase antibody|Membrane metallo endopeptidase variant 1 antibody|Membrane metallo endopeptidase variant 2 antibody|Membrane metalloendopeptidase antibody|Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody|Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody|Membrane metalloendopeptidase variant 1 antibody|Membrane metalloendopeptidase variant 2 antibody|MGC126681 antibody|MGC126707 antibody|MME antibody|NEP antibody|NEP_HUMAN antibody|Neprilysin antibody|Neutral endopeptidase 24.11 antibody|Neutral endopeptidase antibody|SFE antibody|Skin fibroblast elastase antibody
|Pathways||RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration|
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for CD10 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
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|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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