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Mouse (Murine) MME ELISA Kit

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Antigen
Membrane Metallo-Endopeptidase (MME) ELISA Kits
  • 6030454K05Rik
  • AV378681
  • C85356
  • CAK
  • CALLA
  • CD10
  • CD167
  • DDR
  • EDDR1
  • HGK2
  • MCK10
  • MME
  • Mmel
  • Nep
  • NEP
  • NTRK4
  • PTK3
  • PTK3A
  • RTK6
  • SFE
  • TRKE
Epitope
AA 52-750
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
14
11
8
1
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
93.7-6000 pg/mL
Minimum Detection Limit
93.7 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1672816
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32666028 ABIN425104 Sandwich ELISA Serum, Plasma, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.32666028 ABIN1571455 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.813-500 pg/mL 7.813 pg/mL Log in to see
0.32666028 ABIN2708438 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 93.7-6000 pg/mL 93.7 pg/mL Log in to see
0.0012121415 ABIN844316 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012121415 ABIN625421 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 61-15000 pg/mL 61 pg/mL Log in to see
0.0012121415 ABIN2042615 Sandwich ELISA 93.7-6000 pg/mL 93.7 pg/mL Log in to see
0.0012121415 ABIN2684980 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 93.7-6000 pg/mL 93.7 pg/mL Log in to see
0.0012121415 ABIN2044059 Sandwich ELISA 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.0012121415 ABIN2537045 Sandwich ELISA Serum, Plasma, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.0012121415 ABIN1144494 Log in to see
0.0012121415 ABIN2076498 Sandwich ELISA Log in to see

Top referenced MME ELISA Kit for ELISA

Similar MME ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 12 ELISA Kits

General

Antigen Membrane Metallo-Endopeptidase (MME) ELISA Kits
Epitope AA 52-750
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(14), (11), (8), (1), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 93.7-6000 pg/mL
Minimum Detection Limit 93.7 pg/mL
Application ELISA
Pubmed 3 references available
Supplier Log in to see

Product Details MME ELISA Kit

Target details Application Details Handling References for MME Kit (ABIN1672816) Images
Purpose For quantitative detection of mouse CD10 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse CD10
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse CD10 antibod - 1
  • Lyophilized recombinant mouse CD10 standar - 10ng/tubex2
  • Biotinylated anti- mouse CD10 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: Y52-W750

Target details

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Antigen
Alternative Name MME (MME ELISA Kit Abstract)
Background Neprilysin, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), CD10, and common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease, this gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, Neprilysin is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. Neprilysin has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.
Gene ID 17380
UniProt Q61391
Pathways RTK Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse CD10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD10 has been precoated onto 96-well plates. Standards(sf21,Y52-W750) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD10 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m mouse CD10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD10 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 0.59, Standard deviation: 0.037, CV(%): 6.3
  • Sample 2: n=16, Mean(ng/ml): 1.25, Standard deviation: 0.071, CV(%): 5.7
  • Sample 3: n=16, Mean(ng/ml): 3.79, Standard deviation: 0.197, CV(%): 5.2,
  • Sample 1: n=24, Mean(ng/ml): 0.53, Standard deviation: 0.046, CV(%): 8.7
  • Sample 2: n=24, Mean(ng/ml): 1.13, Standard deviation: 0.094, CV(%): 8.3
  • Sample 3: n=24, Mean(ng/ml): 3.85, Standard deviation: 0.277, CV(%): 7.2
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for MME Kit (ABIN1672816)

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Background publications

Tran-Paterson, Willard, Letarte: "The common acute lymphoblastic leukemia antigen (neutral endopeptidase-3.4.24.11) gene is located on human chromosome 3." in: Cancer genetics and cytogenetics, Vol. 42, Issue 1, pp. 129-34, 1989

Barker, Shipp, DAdamio, Masteller, Reinherz: "The common acute lymphoblastic leukemia antigen gene maps to chromosomal region 3 (q21-q27)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 1, pp. 283-7, 1989

Stephenson, Kenny: "The hydrolysis of alpha-human atrial natriuretic peptide by pig kidney microvillar membranes is initiated by endopeptidase-24.11." in: The Biochemical journal, Vol. 243, Issue 1, pp. 183-7, 1987

Images

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Supplier Images
ELISA image for MME ELISA Kit (Membrane Metallo-Endopeptidase) (ABIN1672816) Mouse Neprilysin/CD10 PicoKine ELISA Kit standard curve