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|Application / Reactivity||Human|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (IF)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||24 Antibodies|
|Immunohistochemistry (IHC)||12 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||30 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Western Blotting (WB)||82 Antibodies|
|Antigen||Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R) Antibodies|
|Conjugate||This MST1R antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-MST1R AntibodyTarget Details MST1R Application Details Handling Images
|No Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
|Purification||Tissue culture supernatant|
|Immunogen||A synthetic peptide corresponding to residues near the N-terminus of human Ron was used as an immunogen.|
Target Details MST1RProduct Details anti-MST1R Antibody Application Details Handling Images back to top
|Alternative Name||CD136 / MST1R (MST1R Antibody Abstract)|
Ron, the tyrosine kinase receptor for the Macrophage-stimulating protein, is involved incell dissociation, motility, and matrix invasion (1). Ron, a cDNA homologous to thehepatocyte growth factor (HGF) receptor gene (MET), encodes a putative tyrosinekinase. It has been shown that the Ron gene is expressed in several epithelial tissuesas well as in granulocytes and monocytes. The major RON transcript is translated into aglycosylated single chain precursor, cleaved into a 185 kDa heterodimer (p185Ron) of35 (alpha) and 150 kDa (beta) disulfide-linked chains, before exposure at the cellsurface (2). Alternate Names: anti-c met related tyrosine kinase antibody, anti-CD136 antigen antibody, anti-CDw136antibody, anti-Macrophage stimulating 1 receptor antibody, anti-Macrophage stimulatingprotein receptor alpha chain antibody, anti-MACROPHAGE STIMULATING PROTEINRECEPTOR antibody, anti-Macrophage stimulating protein receptor beta chainantibody, anti-MSP receptor antibody, anti-MST1R antibody, anti-p185 RON antibody,anti-PTK 8 antibody, anti-ptk8 antibody, anti-PTK8 protein tyrosine kinase 8 antibody,anti-RON antibody, anti-RON protein tyrosine kinase antibody, anti-Stem cell derivedtyrosine kinase antibody.
Gene Symbol: MST1R
|Gene ID||4486, 19882, 300999|
Application DetailsProduct Details anti-MST1R Antibody Target Details MST1R Handling Images back to top
|Application Notes||Recommended dilutions: Flow Cytometry 1:40, Immunocytochemistry/Immunofluorescence 1:50-100, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:30, Western Blot 1:1000-10000|
Protocol specific for RON Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-MST1R Antibody Target Details MST1R Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-MST1R Antibody Target Details MST1R Application Details Handling back to top