anti-Cow (Bovine) Neurotrophin 3 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Neurotrophin 3 Antibody (supplied by: Log in to see )

Antigen
Neurotrophin 3 (NTF3) Antibodies
  • NT-3
  • wu:fl98d04
  • hdnf
  • ngf-2
  • ngf2
  • nt-3
  • nt3
  • NTF3
  • HDNF
  • NGF-2
  • NGF2
  • NT3
  • AI316846
  • AI835689
  • Nt3
  • Ntf-3
  • Nts3
  • neurotrophin 3
  • 3'-nucleotidase
  • neurotrophin-3
  • sortilin 1
  • ntf3
  • NTF3
  • NT3
  • Ntf3
  • LOC100345073
  • Sort1
Epitope
N-Term
15
15
13
6
5
5
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus)
172
67
64
8
3
2
2
2
2
1
1
1
Host
Rabbit
109
34
20
8
3
Clonality
Polyclonal
Conjugate
This Neurotrophin 3 antibody is un-conjugated
10
7
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
124
77
53
26
25
18
6
4
4
4
2
1
Supplier
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Catalog No. ABIN1049152
$ 451.00
Plus shipping costs $45.00

General

Antigen Neurotrophin 3 (NTF3) Antibodies
Epitope N-Term
(15), (15), (13), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus)
(172), (67), (64), (8), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(109), (34), (20), (8), (3)
Clonality
Polyclonal
Conjugate This Neurotrophin 3 antibody is un-conjugated
(10), (7), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(124), (77), (53), (26), (25), (18), (6), (4), (4), (4), (2), (1)
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Product Details anti-Neurotrophin 3 Antibody

Target Details Neurotrophin 3 Application Details Handling Images
Brand IHC-plus™
Specificity Human NTF3 / Neurotrophin 3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Opossum (100%) Turkey (93%).
Purification Immunoaffinity purified
Immunogen Synthetic 15 amino acid peptide from N-Terminus of human NTF3 / Neurotrophin 3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Opossum (100%), Turkey (93%).

Type of Immunogen: Synthetic peptide

Target Details Neurotrophin 3

Product Details anti-Neurotrophin 3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name NTF3 / Neurotrophin 3 (NTF3 Antibody Abstract)
Background Name/Gene ID: NTF3
Family: NGF Beta

Synonyms: NTF3, HDNF, NGF2, Nerve growth factor 2, NGF-2, NT-3, Neurotrophic factor, Neurotrophin 3, Neurotrophin-3
Gene ID 4908
Pathways RTK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Neurotrophin 3 Antibody Target Details Neurotrophin 3 Handling Images back to top
Application Notes Approved: IHC-P (5 μg/mL)
Comment

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-Neurotrophin 3 Antibody Target Details Neurotrophin 3 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Expiry Date 12 months

Images

Product Details anti-Neurotrophin 3 Antibody Target Details Neurotrophin 3 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Neurotrophin 3 (NTF3) (N-Term) antibody (ABIN1049152) Anti-NTF3 / Neurotrophin 3 antibody ABIN1049152 IHC staining of human adrenal. Immun...