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|Application / Reactivity||Human|
|Blocking Antibody (Inhibition)||5 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Functional Studies (Func)||2 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (IF)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||2 Antibodies|
|Immunohistochemistry (IHC)||34 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||4 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Neutralization (Neut)||23 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Transfected Cell Culture (TCC)||1 Antibodies|
|Western Blotting (WB)||104 Antibodies|
|Antigen||Neurotrophin 4 (NTF4) Antibodies|
|Conjugate||This Neurotrophin 4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|2 references available|
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Product Details anti-Neurotrophin 4 AntibodyTarget Details Neurotrophin 4 Application Details Handling References for anti-Neurotrophin 4 Antibody (ABIN116200) Images
|Immunogen||Highly purified recombinant human Neutrophin-4.|
Target Details Neurotrophin 4Product Details anti-Neurotrophin 4 Antibody Application Details Handling References for anti-Neurotrophin 4 Antibody (ABIN116200) Images back to top
|Alternative Name||Neurotrophin 4 / NTF4 (NTF4 Antibody Abstract)|
|Background||Neurotrophin 4 (NT4, synonymous with NT5) belongs to the NGF beta family and is a survival factor for peripheral sensory sympathetic neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. NT4 levels are highest in the prostate, with lower levels in thymus, placenta, and skeletal muscle. NT4 is also expressed in embryonic and adult tissues.Synonyms: NT-4, NT-5, NTF5, Neurotrophin 5, Neurotrophin-4, Neurotrophin-5, Neutrophic factor 4|
Application DetailsProduct Details anti-Neurotrophin 4 Antibody Target Details Neurotrophin 4 Handling References for anti-Neurotrophin 4 Antibody (ABIN116200) Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhNT-4 (50.00 ng/mL), a concentration of 2.0-4.0 μg/mL of this antibody is required. ELISA: (Indirect): To detect hNT-4 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hNT-4. (Sandwich): To detect hNT-4 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human NT-4 (PP1059B) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNT-4. Western Blot: To detect hNT-4 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hNT-4 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry (0.5 - 1 μg/mL). (See Protocols)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Protocol||NEUROTROPHIN IMMUNOHISTOCHEMISTRY: The neurotrophins have proved difficult to localize which may be due to masking by, forexample, their association with the trk receptors or very low concentrations. Wheresuccess has been achieved, the conditions required vary greatly for different tissues. Aprotocol has been included for central nervous system tissue and, while it is similar tocommonly used methods, it is important to give strict attention to details such as thoroughwashing, fixative and detergent concentrations, concentration and quality of the primaryantibodies and length of incubations, etc. You may find it possible to use alternateprotocols, however we have experienced many failures using variations of the currentprotocol (and some failures when strict adherence to the procedure is maintained). Asuitable procedure to stain nerve terminals is still being developed. Neurotrophinreceptors have proved much easier to localize. It is recommended that you include a few sections of adult rat cerebellum, spinal cord orkidney in each experiment since these are tissues which are the easiest to stain. Protocol for Immunohistochemistry in the Central Nervous System. At all steps thorough washing is necessary to reduce background. Fixation: Animals are perfused with 1% sodium nitrite in phosphate buffered saline (PBS) (about 50ml) followed by 1 liter of Zamboni's fixative (4% formaldehyde, 15% picric acid in 0. 1Mphosphate buffer). Post fix for no longer than 2 hours. Tissue Preparation: Tissues are removed and washed briefly with PBS followed by cryoprotection in 30%sucrose in PBS overnight at 4°C. 30 µm cryostat sections are cut and washed with agitationinPBS (1x15min), 50% ethanol (3x15min) and PBS (1x15min). Sections can be stored at 4°C in TBS for several weeks in the presence of 0. 2% sodiumazide. Blocking and Primary Antibody Incubations: A 24 well tissue culture plate works well for incubations. Sections are blocked with 20%normal horse serum in PBS for at least 1 hour followed by incubation with primary antibodydiluted in 2xPBS, 0. 3% Triton X-100 containing 0. 02% sodium azide. Incubation can rangefrom 24 hrs to 1 week. Room temperature (RT) is used for 24 hr incubations but 4°C is usedfor longer incubations. Neurotrophin antibodies are normally used after affinity purification at a concentration ofapproximately 0. 5-1. 0 µg/mL. Prepare sufficient antibody only to cover the sections. Secondary Antibodies: Primary antibodies are removed and sections are washed in PBS-T (PBS + 0. 1% Tween),3x15min. Biotinylated affinity purified IgG antibody is then applied to the sections for 2hours at RT followed by another 3x15 min washes in PBS-T. ABC Reagent: The ABC reagent is prepared 30 minutes prior to use and applied to sectionsfor 2 hours at RT according to the manufactures recommendation. ABC reagent is then removed and sections are washed with Tris buffered saline (TBS), 3x15|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Neurotrophin 4 Antibody Target Details Neurotrophin 4 Application Details References for anti-Neurotrophin 4 Antibody (ABIN116200) Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing. Centrifuge vial prior to opening!|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody prior to reconstitution at -20 °C. Following reconstitution store undiluted at 2-8 °C for one month or at -20 °C for longer. Should this product contain a precipitate we recommend microcentrifugation before use.|
References for anti-Neurotrophin 4 Antibody (ABIN116200)Product Details anti-Neurotrophin 4 Antibody Target Details Neurotrophin 4 Application Details Handling Images back to top
Wirth, Patz, Wahle: "Transcellular induction of neuropeptide Y expression by NT4 and BDNF." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 8, pp. 3064-9, 2005 (PubMed).
Miknyoczki, Wan, Chang et al.: "The neurotrophin-trk receptor axes are critical for the growth and progression of human prostatic carcinoma and pancreatic ductal adenocarcinoma xenografts in nude mice." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 8, Issue 6, pp. 1924-31, 2002 (PubMed).