anti-Rat (Rattus) NFAT5 antibody for Western Blotting

Recommended NFAT5 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5) Antibodies
  • zgc:63502
  • wu:fi46f11
  • TonEBP
  • NFAT5
  • nfatz
  • orebp
  • nf-at5
  • nfatl1
  • tonebp
  • DKFZp469P1514
  • AI225870
  • B130038B15Rik
  • CAG-8
  • CAG80
  • NFATL1
  • OREBP
  • mKIAA0827
  • NF-AT5
  • NFATZ
  • TONEBP
  • Nfat
  • nuclear factor of activated T-cells 5, tonicity-responsive
  • nuclear factor of activated T cells 5
  • NFAT5
  • nfat5
  • Nfat5
Epitope
Middle Region
19
7
6
6
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Rabbit, Rat (Rattus)
74
52
36
4
3
3
2
2
2
2
1
Host
Rabbit
64
12
Clonality
Polyclonal
Conjugate
This NFAT5 antibody is un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Western Blotting (WB)
36
30
26
13
12
9
4
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN2780794
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1438015 ABIN2780105 WB Rabbit Middle Region Log in to see Polyclonal 1
3.1438015 ABIN267152 ChIP GS ICC IF IHC IHC (p) IP WB Rabbit IgG Log in to see Polyclonal 2
3.1438015 ABIN3222948 IHC WB Rabbit N-Term Log in to see Polyclonal
3.1438015 ABIN927663 WB Rabbit Middle Region Log in to see Polyclonal
1 ABIN1420798 IHC (p) WB HRP Rabbit IgG pSer1197 Log in to see Polyclonal
1 ABIN1402927 IHC (p) WB Biotin Rabbit IgG pSer1197 Log in to see Polyclonal
1 ABIN1420792 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN1387082 IF (p) IHC (p) WB Rabbit IgG pSer1197 Log in to see Polyclonal
1 ABIN4902005 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1402921 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN1387453 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal

General

Antigen Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5) Antibodies
Epitope Middle Region
(19), (7), (6), (6), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Rabbit, Rat (Rattus)
(74), (52), (36), (4), (3), (3), (2), (2), (2), (2), (1)
Host Rabbit
(64), (12)
Clonality
Polyclonal
Conjugate This NFAT5 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Western Blotting (WB)
(36), (30), (26), (13), (12), (9), (4), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-NFAT5 Antibody

Target Details NFAT5 Application Details Handling References for anti-NFAT5 antibody (ABIN2780794) Images
Blocking Peptide Blocking peptide for this product available: ABIN980637
Sequence PGQPQNEGQP PVTTLLSQQM PENSPLASSI NTNQNIEKID LLVSLQNQGN
Predicted Reactivity Cow: 93%, Dog: 86%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Rabbit: 93%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against NFAT5. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human NFAT5

Target Details NFAT5

Product Details anti-NFAT5 Antibody Application Details Handling References for anti-NFAT5 antibody (ABIN2780794) Images back to top
Antigen
Alternative Name NFAT5 (NFAT5 Antibody Abstract)
Background NFAT5 is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements.The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: KIAA0827, NF-AT5, NFATL1, NFATZ, OREBP, TONEBP
Protein Interaction Partner: gag-pol, SUMO2, TBC1D4, NFAT5, FOXP3,
Protein Size: 1531
Molecular Weight 166 kDa
Gene ID 10725
NCBI Accession NM_006599, NP_006590
UniProt O94916
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Adaptive Immunity
Pathways RTK Signaling, WNT Signaling

Application Details

Product Details anti-NFAT5 Antibody Target Details NFAT5 Handling References for anti-NFAT5 antibody (ABIN2780794) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1531 AA

Restrictions For Research Use only

Handling

Product Details anti-NFAT5 Antibody Target Details NFAT5 Application Details References for anti-NFAT5 antibody (ABIN2780794) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NFAT5 antibody (ABIN2780794)

Product Details anti-NFAT5 Antibody Target Details NFAT5 Application Details Handling Images back to top
Background publications

Zhou, Ferraris, Dmitrieva, Liu, Burg: "MKP-1 inhibits high NaCl-induced activation of p38 but does not inhibit the activation of TonEBP/OREBP: opposite roles of p38alpha and p38delta." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 14, pp. 5620-5, 2008

Images

Product Details anti-NFAT5 Antibody Target Details NFAT5 Application Details Handling References for anti-NFAT5 antibody (ABIN2780794) back to top
Supplier Images
Western Blotting (WB) image for anti-Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5) (Middle Region) antibody (ABIN2780794) WB Suggested Anti-NFAT5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: