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anti-Rat (Rattus) NFAT5 antibody for Western Blotting

Recommended NFAT5 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5) Antibodies
  • AI225870
  • B130038B15Rik
  • CAG-8
  • CAG80
  • DKFZp469P1514
  • mKIAA0827
  • nf-at5
  • NF-AT5
  • Nfat
  • NFAT5
  • NFATL1
  • nfatl1
  • NFATZ
  • nfatz
  • OREBP
  • orebp
  • tonebp
  • TONEBP
  • TonEBP
  • wu:fi46f11
  • zgc:63502
Epitope
Middle Region
16
7
7
6
3
2
2
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
65
43
29
Host
Rabbit
55
12
Clonality
Polyclonal
Conjugate
This NFAT5 antibody is un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Western Blotting (WB)
34
29
22
11
10
4
2
Supplier
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Catalog No. ABIN2780105
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN927663 WB Rabbit Middle Region Log in to see Polyclonal
0.0011806369 ABIN1420798 IHC (p) WB HRP Rabbit IgG pSer1197 Log in to see Polyclonal
0.0011806369 ABIN1402927 IHC (p) WB Biotin Rabbit IgG pSer1197 Log in to see Polyclonal
0.0011806369 ABIN1420792 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
0.0011806369 ABIN1387082 IF (p) IHC (p) WB Rabbit IgG pSer1197 Log in to see Polyclonal
0.0011806369 ABIN1402921 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
0.0011806369 ABIN1387453 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0011806369 ABIN3222948 IHC WB Rabbit N-Term Log in to see Polyclonal

Top referenced anti-NFAT5 antibody for Western Blotting

Similar anti-NFAT5 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 34 Antibodies 16 Antibodies 9 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies 7 Antibodies 6 Antibodies
Immunohistochemistry (IHC) 12 Antibodies 6 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies 22 Antibodies
Immunofluorescence (IF) 5 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies

General

Antigen Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5) Antibodies
Epitope Middle Region
(16), (7), (7), (6), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(65), (43), (29)
Host Rabbit
(55), (12)
Clonality
Polyclonal
Conjugate This NFAT5 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Western Blotting (WB)
(34), (29), (22), (11), (10), (4), (2)
Pubmed 1 reference available
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Product Details anti-NFAT5 Antibody

Target Details NFAT5 Application Details Handling References for anti-NFAT5 Antibody (ABIN2780105) Images
Blocking Peptide Blocking peptide for this product available: ABIN980636
Sequence TIQTQDISQP GTFPAVSAAS QLPSSDALLQ QATQFQTREA QSRDTIQSDT
Predicted Reactivity Dog : 100 %, Mouse : 100 %, Bovine : 90 %, Horse : 90 %, Human : 90 %, Pig : 90 %, Rat : 90 %, Rabbit : 80 %
Characteristics This is a rabbit polyclonal antibody against NFAT5. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of mouse NFAT5

Target Details NFAT5

Product Details anti-NFAT5 Antibody Application Details Handling References for anti-NFAT5 Antibody (ABIN2780105) Images back to top
Antigen
Alternative Name NFAT5 (NFAT5 Antibody Abstract)
Background Nfat5 contains 1 RHD (Rel-like) domain, and plays a role in the inducible expression of genes. Nfat5 regulates hypertonicity-induced cellular accumulation of osmolytes. Nfat5 might be capable of forming stable dimers with DNA elements
Molecular Weight 166 kDa
Gene ID 54446
NCBI Accession NM_018823
UniProt Q9JIH4
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Adaptive Immunity
Pathways RTK Signaling, WNT Signaling

Application Details

Product Details anti-NFAT5 Antibody Target Details NFAT5 Handling References for anti-NFAT5 Antibody (ABIN2780105) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1534 AA

Restrictions For Research Use only

Handling

Product Details anti-NFAT5 Antibody Target Details NFAT5 Application Details References for anti-NFAT5 Antibody (ABIN2780105) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-NFAT5 Antibody (ABIN2780105)

Product Details anti-NFAT5 Antibody Target Details NFAT5 Application Details Handling Images back to top
Background publications

Pan, Tsuruta, Masuda, Imamura, Bazan, Arai, Arai, Miyatake: "NFATz: a novel rel similarity domain containing protein." in: Biochemical and biophysical research communications, Vol. 272, Issue 3, pp. 765-76, 2000

Images

Product Details anti-NFAT5 Antibody Target Details NFAT5 Application Details Handling References for anti-NFAT5 Antibody (ABIN2780105) back to top
Supplier Images
Western Blotting (WB) image for anti-NFAT5 antibody (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive) (Middle Region) (ABIN2780105) WB Suggested Anti-NFAT5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Western Blotting (WB) image for anti-NFAT5 antibody (Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive) (Middle Region) (ABIN2780105) Lanes: Lane 1:241 µg HT 29 lysate blocked with 5 % FBS Lane 2: 041 µg HT 29 lysate bl...