You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human NFATC1 antibody for Enzyme Immunoassay

Recommended NFATC1 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) Antibodies
  • 2210017P03Rik
  • AI449492
  • AV076380
  • NF-ATc
  • nf-atc
  • NF-ATC
  • NF-ATc1
  • NFAT
  • NFAT2
  • nfat2
  • nfatc
  • NFATc
  • NFATC1
  • Nfatcb
  • NFATmac
  • si:ch211-198c22.2
Epitope
AA 428-716
28
25
14
13
13
7
6
6
6
5
5
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
142
34
30
2
1
1
1
Host
Mouse
96
45
1
Clonality
Monoclonal
Conjugate
This NFATC1 antibody is un-conjugated
5
4
4
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
113
52
38
12
11
8
7
6
5
3
2
2
2
2
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN336112
$ 412.50
Plus shipping costs $45.00

Top referenced anti-NFATC1 antibody for Enzyme Immunoassay

  • Human Monoclonal NFATC1 Primary Antibody for EIA, IHC (p) - ABIN336112 (2 Publications): Akimzhanov, Krenacs, Schlegel, Klein-Hessling, Bagdi, Stelkovics, Kondo, Chuvpilo, Wilke, Avots, Gattenlöhner, Müller-Hermelink, Palmetshofer, Serfling: Epigenetic changes and suppression of the nuclear factor of activated T cell 1 (NFATC1) promoter in human lymphomas with defects in immunoreceptor signaling. in The American journal of pathology 2008 (PubMed)

Similar anti-NFATC1 Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 3 Antibodies
ELISA 53 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 7 Antibodies
Functional Studies (Func) 2 Antibodies
Gel Shift (GS) 3 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 39 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Western Blotting (WB) 116 Antibodies

General

Antigen Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) Antibodies
Epitope AA 428-716
(28), (25), (14), (13), (13), (7), (6), (6), (6), (5), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(142), (34), (30), (2), (1), (1), (1)
Host Mouse
(96), (45), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFATC1 antibody is un-conjugated
(5), (4), (4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(113), (52), (38), (12), (11), (8), (7), (6), (5), (3), (2), (2), (2), (2)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-NFATC1 Antibody

Target Details NFATC1 Application Details Handling References for anti-NFATC1 Antibody (ABIN336112) Images
Specificity The antibody recognizes Human NFATc1. Other species not tested.
Characteristics Synonyms: NF-ATc1, NFATC1, NFATC, Nuclear factor of activated T-cells, cytoplasmic 1
Purification Protein-G affinity chromatography
Immunogen Recombinant human NFATc1 (aa 428-716) purified from E. coli
Clone AT1C3
Isotype IgG2a

Target Details NFATC1

Product Details anti-NFATC1 Antibody Application Details Handling References for anti-NFATC1 Antibody (ABIN336112) Images back to top
Antigen
Alternative Name NFAT2 / NFATC (NFATC1 Antibody Abstract)
Background Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATc1) is a component of the nuclear factor of activated T cells DNA-binding transcription complex which consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. This protein plays a main role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription and functions as a major molecular target for the immunosuppressive drugs such as cyclosporine A. NFATc1 is expressed in most human primary lymphocytes and mature human T- and B-cell neoplasms.Synonyms: NF-ATc1, NFATC, NFATC1, Nuclear factor of activated T-cells, cytoplasmic 1
Gene ID 4772
UniProt O95644
Pathways RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFATC1 Antibody Target Details NFATC1 Handling References for anti-NFATC1 Antibody (ABIN336112) Images back to top
Application Notes ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This NFATC1 antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-NFATC1 Antibody Target Details NFATC1 Application Details References for anti-NFATC1 Antibody (ABIN336112) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, containing 0.09 % Sdium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-NFATC1 Antibody (ABIN336112)

Product Details anti-NFATC1 Antibody Target Details NFATC1 Application Details Handling Images back to top
Background publications

Akimzhanov, Krenacs, Schlegel, Klein-Hessling, Bagdi, Stelkovics, Kondo, Chuvpilo, Wilke, Avots, Gattenlöhner, Müller-Hermelink, Palmetshofer, Serfling: "Epigenetic changes and suppression of the nuclear factor of activated T cell 1 (NFATC1) promoter in human lymphomas with defects in immunoreceptor signaling." in: The American journal of pathology, Vol. 172, Issue 1, pp. 215-24, 2008

Asagiri, Sato, Usami, Ochi, Nishina, Yoshida, Morita, Wagner, Mak, Serfling, Takayanagi: "Autoamplification of NFATc1 expression determines its essential role in bone homeostasis." in: The Journal of experimental medicine, Vol. 202, Issue 9, pp. 1261-9, 2005

Images

Product Details anti-NFATC1 Antibody Target Details NFATC1 Application Details Handling References for anti-NFATC1 Antibody (ABIN336112) back to top
Supplier Images
Western Blotting (WB) image for anti-NFATC1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1) (AA 428-716) (ABIN336112) anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (AA 428-716) antibody
Immunohistochemistry (IHC) image for anti-NFATC1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1) (AA 428-716) (ABIN336112) anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (AA 428-716) antibody (Image 2)
Western Blotting (WB) image for anti-NFATC1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1) (AA 428-716) (ABIN336112) anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) (AA 428-716) antibody (Image 3)