|Antigen||Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) Antibodies|
|Epitope||AA 428-716 Alternatives|
|Conjugate||This NFATC1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
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Product Details anti-NFATC1 AntibodyTarget Details NFATC1 Application Details Handling References for anti-NFATC1 antibody (ABIN336112) Images
|Specificity||The antibody recognizes Human NFATc1. Other species not tested.|
|Characteristics||Synonyms: NF-ATc1, NFATC1, NFATC, Nuclear factor of activated T-cells, cytoplasmic 1|
|Purification||Protein-G affinity chromatography|
|Immunogen||Recombinant human NFATc1 (aa 428-716) purified from E. coli|
Target Details NFATC1Product Details anti-NFATC1 Antibody Application Details Handling References for anti-NFATC1 antibody (ABIN336112) Images back to top
|Alternative Name||NFAT2 / NFATC (NFATC1 Antibody Abstract)|
|Background||Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATc1) is a component of the nuclear factor of activated T cells DNA-binding transcription complex which consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. This protein plays a main role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription and functions as a major molecular target for the immunosuppressive drugs such as cyclosporine A. NFATc1 is expressed in most human primary lymphocytes and mature human T- and B-cell neoplasms.Synonyms: NF-ATc1, NFATC, NFATC1, Nuclear factor of activated T-cells, cytoplasmic 1|
|Pathways||RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-NFATC1 Antibody Target Details NFATC1 Handling References for anti-NFATC1 antibody (ABIN336112) Images back to top
ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This NFATC1 antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-NFATC1 Antibody Target Details NFATC1 Application Details References for anti-NFATC1 antibody (ABIN336112) Images back to top
|Buffer||PBS, pH 7.4, containing 0.09 % Sdium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-NFATC1 antibody (ABIN336112)Product Details anti-NFATC1 Antibody Target Details NFATC1 Application Details Handling Images back to top
Akimzhanov, Krenacs, Schlegel, Klein-Hessling, Bagdi, Stelkovics, Kondo, Chuvpilo, Wilke, Avots, Gattenlöhner, Müller-Hermelink, Palmetshofer, Serfling: "Epigenetic changes and suppression of the nuclear factor of activated T cell 1 (NFATC1) promoter in human lymphomas with defects in immunoreceptor signaling." in: The American journal of pathology, Vol. 172, Issue 1, pp. 215-24, 2008
Asagiri, Sato, Usami, Ochi, Nishina, Yoshida, Morita, Wagner, Mak, Serfling, Takayanagi: "Autoamplification of NFATc1 expression determines its essential role in bone homeostasis." in: The Journal of experimental medicine, Vol. 202, Issue 9, pp. 1261-9, 2005
ImagesProduct Details anti-NFATC1 Antibody Target Details NFATC1 Application Details Handling References for anti-NFATC1 antibody (ABIN336112) back to top