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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||96 Antibodies||16 Antibodies||14 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies||2 Antibodies||2 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies||2 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||14 Antibodies||4 Antibodies||4 Antibodies|
|Immunohistochemistry (IHC)||39 Antibodies||11 Antibodies||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies||11 Antibodies|
|Immunofluorescence (IF)||7 Antibodies|
|Antigen||Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) Antibodies|
|Epitope||AA 197-304 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This NFATC1 antibody is conjugated to Alexa Fluor 488 Alternatives|
Flow Cytometry (FACS), Mass Cytometry (CyTOF)
|7 references available|
|Supplier||Log in to see|
Product Details anti-NFATC1 AntibodyTarget Details NFATC1 Application Details Handling References for anti-NFATC1 Antibody (ABIN2657092) Images
|Purification||The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.|
Target Details NFATC1Product Details anti-NFATC1 Antibody Application Details Handling References for anti-NFATC1 Antibody (ABIN2657092) Images back to top
|Alternative Name||NFATc1 (NFATC1 Antibody Abstract)|
|Background||The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. The protein complex consists of NFAT1, NFAT2 (NFATc1 or NFATc), NFAT3, and NFAT4. All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response. NFAT proteins are predominantly expressed in cells of the immune system but are also expressed in skeletal muscle, keratinocytes and adipocytes, regulating cell differentiation programs in these cells. In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.|
|Pathways||RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-NFATC1 Antibody Target Details NFATC1 Handling References for anti-NFATC1 Antibody (ABIN2657092) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-NFATC1 Antibody Target Details NFATC1 Application Details References for anti-NFATC1 Antibody (ABIN2657092) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Protect from prolonged exposure to light. Do not freeze.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C|
References for anti-NFATC1 Antibody (ABIN2657092)Product Details anti-NFATC1 Antibody Target Details NFATC1 Application Details Handling Images back to top
Brandt, Liman, Bendfeldt et al.: "Whole blood flow cytometric measurement of NFATc1 and IL-2 expression to analyze cyclosporine A-mediated effects in T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 77, Issue 7, pp. 607-13, 2010 (PubMed).
Zhao, Wang, Liu et al.: "NFATc1: functions in osteoclasts." in: The international journal of biochemistry & cell biology, Vol. 42, Issue 5, pp. 576-9, 2010 (PubMed).
Serfling, Berberich-Siebelt, Avots: "NFAT in lymphocytes: a factor for all events?" in: Science's STKE : signal transduction knowledge environment, Vol. 2007, Issue 398, pp. pe42, 2007 (PubMed).
Hogan, Chen, Nardone et al.: "Transcriptional regulation by calcium, calcineurin, and NFAT." in: Genes & development, Vol. 17, Issue 18, pp. 2205-32, 2003 (PubMed).
Timmerman, Healy, Ho et al.: "Redundant expression but selective utilization of nuclear factor of activated T cells family members." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2735-40, 1997 (PubMed).
Northrop, Ullman, Crabtree: "Characterization of the nuclear and cytoplasmic components of the lymphoid-specific nuclear factor of activated T cells (NF-AT) complex." in: The Journal of biological chemistry, Vol. 268, Issue 4, pp. 2917-23, 1993 (PubMed).
Hoey, Sun, Williamson et al.: "Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins." in: Immunity, Vol. 2, Issue 5, pp. 461-72, 1995 (PubMed).
ImagesProduct Details anti-NFATC1 Antibody Target Details NFATC1 Application Details Handling References for anti-NFATC1 Antibody (ABIN2657092) back to top