anti-Human NTRK3 antibody for Immunocytochemistry

Recommended NTRK3 Antibody (supplied by: Log in to see )

Antigen
Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) Antibodies
  • TRKC
  • gp145(trkC)
  • AW125844
  • Ntrk3_tv3
  • TrkC
  • trkC
  • neurotrophic tyrosine kinase, receptor, type 3
  • NTRK3
  • LOC100227403
  • Ntrk3
Epitope
AA 50-150
38
18
14
13
10
8
8
8
8
5
5
5
4
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
173
87
50
5
3
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
116
66
16
13
6
Clonality
Polyclonal
Conjugate
This NTRK3 antibody is un-conjugated
9
5
5
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
165
87
78
27
23
13
7
2
2
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN409292
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1499842 ICC IF IHC (p) WB Mouse IgG1 Log in to see 24B3
1 ABIN4362589 ICC IF IHC IHC (p) WB Mouse IgG1 Transcript Variant 3 Log in to see 2B8
1 ABIN5080309 ICC IF Rabbit IgG Log in to see Polyclonal
1 ABIN5080310 ICC IF Rabbit IgG Log in to see Polyclonal
1 ABIN4362592 ICC IF IHC IHC (p) Rabbit AA 300-400 Log in to see Polyclonal
1 ABIN4362591 ICC IF IHC IHC (p) Rabbit AA 300-400 Log in to see Polyclonal
1 ABIN5014005 ICC IHC IP WB Rabbit IgG AA 32-429 Log in to see Polyclonal

Similar anti-NTRK3 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Interaction (receptor-ligand) (BI (rec-lig)) 1 Antibodies 1 Antibodies
ELISA 8 Antibodies 25 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 3 Antibodies
Immunofluorescence (IF) 1 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 47 Antibodies

General

Antigen Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) Antibodies
Epitope AA 50-150
(38), (18), (14), (13), (10), (8), (8), (8), (8), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(173), (87), (50), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(116), (66), (16), (13), (6)
Clonality
Polyclonal
Conjugate This NTRK3 antibody is un-conjugated
(9), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
(165), (87), (78), (27), (23), (13), (7), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-NTRK3 Antibody

Target Details NTRK3 Application Details Handling Images
Purification Unpurified
Immunogen A synthetic peptide made to the extracellular domain of the human TrkC protein (within residues 50-150). [UniProt Q16288].

Target Details NTRK3

Product Details anti-NTRK3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name TrkC (NTRK3 Antibody Abstract)
Background Gene Symbol: NTRK3
Gene ID 4916
UniProt Q16288, Q6VNS1, Q03351
Research Area Cancer
Pathways RTK Signaling, Neurotrophin Signaling Pathway, Regulation of Cell Size

Application Details

Product Details anti-NTRK3 Antibody Target Details NTRK3 Handling Images back to top
Application Notes Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:1000, Immunohistochemistry-Paraffin 1:200This TrkC antibody is useful for Immunohistochemistry-Paraffin and Immunocytochemistry/ Immunofluorescence.In ICC/IF, membrane staining was observed in neuro2a cells. In IHC-P, staining was observed in the membrane of mouse brain and spinal cord. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunocytochemistry Protocol specific for TrkC antibody Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.Immunohistochemistry Protocol specific for TrkC antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only

Handling

Product Details anti-NTRK3 Antibody Target Details NTRK3 Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitute with 0.1 mL sterilized water to desired concentration. Centrifuge to remove any insoluble material.
Buffer Whole antisera
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Images

Product Details anti-NTRK3 Antibody Target Details NTRK3 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) anti-Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) (AA 50-150) antibody
Immunohistochemistry (IHC) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) anti-Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) (AA 50-150) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) anti-Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) (AA 50-150) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) anti-Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) (AA 50-150) antibody (Image 4)
Immunohistochemistry (IHC) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) anti-Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) (AA 50-150) antibody (Image 5)
Immunohistochemistry (IHC) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) anti-Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) (AA 50-150) antibody (Image 6)
Immunohistochemistry (IHC) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) anti-Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3) (AA 50-150) antibody (Image 7)
Immunofluorescence (IF) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) Immunocytochemistry/Immunofluorescence: TrkC Antibody [ABIN4092929] - IF on rat DRG a...
Immunofluorescence (IF) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) Immunocytochemistry/Immunofluorescence: TrkC Antibody [ABIN4092929] - Rat DRG at 1 : ...
Immunofluorescence (IF) image for anti-NTRK3 antibody (Neurotrophic tyrosine Kinase, Receptor, Type 3) (AA 50-150) (ABIN409292) Immunocytochemistry/Immunofluorescence: TrkC Antibody [ABIN4092929] - TrkC antibody w...