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anti-Dog (Canine) PAK4 antibody for Western Blotting

Recommended PAK4 Antibody (supplied by: Log in to see )

Antigen
P21-Activated Kinase 4 (PAK4) Antibodies
  • 5730488L07Rik
  • AW555722
  • mKIAA1142
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
163
58
57
14
3
2
1
1
Host
Mouse
141
24
2
Clonality
Monoclonal
Conjugate
This PAK4 antibody is un-conjugated
11
11
10
5
5
5
3
3
3
3
3
3
3
3
2
Application
Western Blotting (WB)
121
65
40
32
20
18
6
6
3
1
1
Supplier
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Catalog No. ABIN492621
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011806369 ABIN1585668 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal

Similar anti-PAK4 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine)
Western Blotting (WB) 120 Antibodies 34 Antibodies 28 Antibodies 2 Antibodies
Immunoprecipitation (IP) 6 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies 11 Antibodies 10 Antibodies
Immunohistochemistry (IHC) 39 Antibodies 11 Antibodies 8 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 32 Antibodies 21 Antibodies 32 Antibodies
Immunofluorescence (IF) 6 Antibodies

General

Antigen P21-Activated Kinase 4 (PAK4) Antibodies
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(163), (58), (57), (14), (3), (2), (1), (1)
Host Mouse
(141), (24), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PAK4 antibody is un-conjugated
(11), (11), (10), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Western Blotting (WB)
(121), (65), (40), (32), (20), (18), (6), (6), (3), (1), (1)
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Product Details anti-PAK4 Antibody

Target Details PAK4 Application Details Handling Images
Specificity Recognizes p21 activated kinase 4 (PAK4).
Characteristics Synonyms: PAK 4, PAK-4, p21-activated kinase 4, KIAA1142, Serine/threonine-protein kinase PAK 4
Purification Subsequent Ultrafiltration and Size Exclusion Chromatography.
Components incl. positive Control
Immunogen Peptide conjugated to hemocyaninEpitope: Peptide derived from the kinase activation loop.
Clone 6C1
Isotype IgG1

Target Details PAK4

Product Details anti-PAK4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PAK4 (PAK4 Antibody Abstract)
Background P21 activated kinases (PAK) are regulated by the small GTP - binding proteins Rac and cdc42, which stimulate autophosphorylation and phosphorylation of downstream signaling proteins. PAK4 is activated upon autophosphorylation of Ser 474 in the kinase activation loop. PAK4 is frequently overexpressed in human tumor cell lines and might play an important role in oncogenesis.Synonyms: KIAA1142, PAK 4, PAK-4, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4
Gene ID 10298
UniProt O96013
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways RTK Signaling

Application Details

Product Details anti-PAK4 Antibody Target Details PAK4 Handling Images back to top
Application Notes Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SW480 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive Control Provided. Cell lysate from untreated SW480 cellsDescription: Cell lysate from untreated SW480 cells, colorectal adenocarcinoma (Human)Format: Lyophilized cell lysate from serum starved SW480 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
Restrictions For Research Use only

Handling

Product Details anti-PAK4 Antibody Target Details PAK4 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Expiry Date 12 months

Images

Product Details anti-PAK4 Antibody Target Details PAK4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PAK4 antibody (P21-Activated Kinase 4) (ABIN492621) anti-P21-Activated Kinase 4 (PAK4) antibody