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anti-Dog (Canine) PAK4 antibody for Western Blotting

Recommended PAK4 Antibody (supplied by: Log in to see )

Antigen
P21-Activated Kinase 4 (PAK4) Antibodies
  • 5730488L07Rik
  • AW555722
  • mKIAA1142
  • p21 protein (Cdc42/Rac)-activated kinase 4
  • p21-activated kinase 4
  • PAK4
  • LOC100336246
  • Pak4
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
175
87
71
17
6
6
4
3
3
2
2
1
1
1
1
Host
Mouse
149
25
2
Clonality
Monoclonal
Conjugate
This PAK4 antibody is un-conjugated
11
11
10
5
5
5
3
3
3
3
3
3
3
3
2
1
1
Application
Western Blotting (WB)
134
66
44
34
21
19
10
6
5
1
1
1
Supplier
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Catalog No. ABIN492621
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN610860 WB Rabbit AA 23-72 Log in to see Polyclonal 2
1 ABIN1585668 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1837112 WB Rabbit AA 268-317 Log in to see Polyclonal
1 ABIN1921164 IP WB Rabbit IgG AA 25-75 Log in to see Polyclonal

Top referenced anti-PAK4 antibody for Western Blotting

  • Bat Polyclonal PAK4 Primary Antibody for WB - ABIN610860 (2 Publications): Jones, Jakubowicz, Pitossi, Maurer, Hemmings: Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. in Proceedings of the National Academy of Sciences of the United States of America 1991 (PubMed)

Similar anti-PAK4 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine)
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 134 Antibodies 61 Antibodies 40 Antibodies 5 Antibodies
Immunoprecipitation (IP) 6 Antibodies 3 Antibodies 3 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies 12 Antibodies 10 Antibodies
Immunohistochemistry (IHC) 43 Antibodies 21 Antibodies 11 Antibodies 1 Antibodies

General

Antigen P21-Activated Kinase 4 (PAK4) Antibodies
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(175), (87), (71), (17), (6), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(149), (25), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PAK4 antibody is un-conjugated
(11), (11), (10), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1)
Application Western Blotting (WB)
(134), (66), (44), (34), (21), (19), (10), (6), (5), (1), (1), (1)
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Product Details anti-PAK4 Antibody

Target Details PAK4 Application Details Handling Images
Specificity Recognizes p21 activated kinase 4 (PAK4).
Characteristics Synonyms: PAK 4, PAK-4, p21-activated kinase 4, KIAA1142, Serine/threonine-protein kinase PAK 4
Purification Subsequent Ultrafiltration and Size Exclusion Chromatography.
Components incl. positive Control
Immunogen Peptide conjugated to hemocyaninEpitope: Peptide derived from the kinase activation loop.
Clone 6C1
Isotype IgG1

Target Details PAK4

Product Details anti-PAK4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PAK4 (PAK4 Antibody Abstract)
Background P21 activated kinases (PAK) are regulated by the small GTP - binding proteins Rac and cdc42, which stimulate autophosphorylation and phosphorylation of downstream signaling proteins. PAK4 is activated upon autophosphorylation of Ser 474 in the kinase activation loop. PAK4 is frequently overexpressed in human tumor cell lines and might play an important role in oncogenesis.Synonyms: KIAA1142, PAK 4, PAK-4, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4
Gene ID 10298
UniProt O96013
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways RTK Signaling

Application Details

Product Details anti-PAK4 Antibody Target Details PAK4 Handling Images back to top
Application Notes Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SW480 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive Control Provided. Cell lysate from untreated SW480 cellsDescription: Cell lysate from untreated SW480 cells, colorectal adenocarcinoma (Human)Format: Lyophilized cell lysate from serum starved SW480 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
Restrictions For Research Use only

Handling

Product Details anti-PAK4 Antibody Target Details PAK4 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Expiry Date 12 months

Images

Product Details anti-PAK4 Antibody Target Details PAK4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PAK4 antibody (P21-Activated Kinase 4) (ABIN492621) anti-P21-Activated Kinase 4 (PAK4) antibody