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anti-Rabbit PAK4 antibody for Western Blotting

Recommended PAK4 Antibody (supplied by: Log in to see )

Antigen
P21-Activated Kinase 4 (PAK4) Antibodies
  • 5730488L07Rik
  • AW555722
  • mKIAA1142
  • p21 protein (Cdc42/Rac)-activated kinase 4
  • p21-activated kinase 4
  • PAK4
  • LOC100336246
  • Pak4
Epitope
AA 23-72
28
16
15
12
11
6
6
5
5
3
3
3
2
2
2
2
1
1
1
1
1
Reactivity
Bat, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
171
84
71
16
5
5
4
2
2
2
1
1
1
Host
Rabbit
144
26
2
Clonality
Polyclonal
Conjugate
This PAK4 antibody is un-conjugated
11
11
10
5
5
5
3
3
3
3
3
3
3
3
2
1
1
Application
Western Blotting (WB)
131
64
42
34
21
18
10
6
5
1
1
1
Supplier
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Catalog No. ABIN610860
$ 551.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1585668 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN747505 IHC (p) WB Biotin Mouse IgG Log in to see Polyclonal
1 ABIN747512 IHC (p) WB HRP Mouse IgG Log in to see Polyclonal
1 ABIN1837112 WB Rabbit AA 268-317 Log in to see Polyclonal
1 ABIN747503 IF (p) IHC (p) WB Mouse IgG Log in to see Polyclonal
1 ABIN1921164 IP WB Rabbit IgG AA 25-75 Log in to see Polyclonal

Top referenced anti-PAK4 antibody for Western Blotting

  • Bat Polyclonal PAK4 Primary Antibody for WB - ABIN610860 (2 Publications): Jones, Jakubowicz, Pitossi, Maurer, Hemmings: Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. in Proceedings of the National Academy of Sciences of the United States of America 1991 (PubMed)

Similar anti-PAK4 Antibodies

Application / Reactivity Cow (Bovine) Human Mouse (Murine) Rat (Rattus) Hamster Dog (Canine) Rabbit Monkey Bat Guinea Pig Horse (Equine) Zebrafish
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 131 Antibodies 59 Antibodies 40 Antibodies 6 Antibodies 5 Antibodies 7 Antibodies 6 Antibodies 3 Antibodies 2 Antibodies 3 Antibodies 1 Antibodies 1 Antibodies
Immunoprecipitation (IP) 6 Antibodies 3 Antibodies 3 Antibodies

General

Antigen P21-Activated Kinase 4 (PAK4) Antibodies
Epitope AA 23-72
(28), (16), (15), (12), (11), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Bat, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(171), (84), (71), (16), (5), (5), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(144), (26), (2)
Clonality
Polyclonal
Conjugate This PAK4 antibody is un-conjugated
(11), (11), (10), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1)
Application Western Blotting (WB)
(131), (64), (42), (34), (21), (18), (10), (6), (5), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-PAK4 Antibody

Target Details PAK4 Application Details Handling References for anti-PAK4 Antibody (ABIN610860) Images
Specificity Rat PAK4
Predicted Reactivity Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse (100%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide located between aa23-72 of rat Pak4 (B5DF62, NP_001099708). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig (100%), Galago, Xenopus (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Target Details PAK4

Product Details anti-PAK4 Antibody Application Details Handling References for anti-PAK4 Antibody (ABIN610860) Images back to top
Antigen
Alternative Name PAK4 (PAK4 Antibody Abstract)
Background Name/Gene ID: PAK4
Subfamily: PAK (STE20)
Family: Protein Kinase

Synonyms: PAK4, KIAA1142, p21(CDKN1A)-activated kinase 4, PAK-4, p21-activated kinase 4
Gene ID 10298
NCBI Accession NP_001099708
UniProt O96013
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways RTK Signaling

Application Details

Product Details anti-PAK4 Antibody Target Details PAK4 Handling References for anti-PAK4 Antibody (ABIN610860) Images back to top
Application Notes Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Restrictions For Research Use only

Handling

Product Details anti-PAK4 Antibody Target Details PAK4 Application Details References for anti-PAK4 Antibody (ABIN610860) Images back to top
Format Lyophilized
Reconstitution Distilled water
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.

References for anti-PAK4 Antibody (ABIN610860)

Product Details anti-PAK4 Antibody Target Details PAK4 Application Details Handling Images back to top
Product cited in:

Marte, Downward: "PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond." in: Trends in biochemical sciences, Vol. 22, Issue 9, pp. 355-8, 1997

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991

Images

Product Details anti-PAK4 Antibody Target Details PAK4 Application Details Handling References for anti-PAK4 Antibody (ABIN610860) back to top
Supplier Images
 image for anti-PAK4 antibody (P21-Activated Kinase 4) (AA 23-72) (ABIN610860) anti-P21-Activated Kinase 4 (PAK4) (AA 23-72) antibody