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anti-Human PDGFA antibody for Functional Studies

Recommended PDGFA Antibody (supplied by: Log in to see )

Antigen
Platelet-Derived Growth Factor alpha Polypeptide (PDGFA) Antibodies
  • pdgf
  • PDGF-A
  • pdgf-a
  • PDGF1
  • pdgf1
  • pdgfa
  • pdgfa-2
  • PDGFACP
  • zgc:100777
Reactivity
Human
88
38
37
2
1
1
1
Host
Rabbit
78
6
5
3
Clonality
Polyclonal
Conjugate
This PDGFA antibody is un-conjugated
7
5
4
2
2
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
65
35
31
22
16
5
4
3
3
2
2
2
2
1
1
Supplier
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Catalog No. ABIN116207
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087631686 ABIN116208 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
0.00087631686 ABIN2476090 Func IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.00087631686 ABIN2476091 Func IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 3
0.00087631686 ABIN470616 Func WB Goat IgG Log in to see Polyclonal
0.00087631686 ABIN2476092 Func IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 2

Top referenced anti-PDGFA antibody for Functional Studies

Similar anti-PDGFA Antibodies

Application / Reactivity Human
Dot Blot (DB) 3 Antibodies
ELISA 33 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Functional Studies (Func) 6 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 31 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Neutralization (Neut) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 65 Antibodies

General

Antigen Platelet-Derived Growth Factor alpha Polypeptide (PDGFA) Antibodies
Reactivity Human
(88), (38), (37), (2), (1), (1), (1)
Host Rabbit
(78), (6), (5), (3)
Clonality
Polyclonal
Conjugate This PDGFA antibody is un-conjugated
(7), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(65), (35), (31), (22), (16), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1)
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Product Details anti-PDGFA Antibody

Target Details PDGFA Application Details Handling Images
Specificity The antibody recognizes the human Platelet Derived Growth Factor AA (hPDGF-AA). Not tested in other species.
Purification Immunoaffinity chromatography using immobilized human PDGF matrix
Immunogen Highly pure (>98%) recombinant hPDGF-AA

Target Details PDGFA

Product Details anti-PDGFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name PDGFA (PDGFA Antibody Abstract)
Background Synonyms: PDGF A, PDGF alpha, PDGF subunit A, PDGF-1, PDGF-A, PDGF1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha, Platelet-derived growth factor subunit A
Gene ID 5154
NCBI Accession NP_002598
UniProt P04085
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PDGFA Antibody Target Details PDGFA Handling Images back to top
Application Notes Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-AA (5.0 ng/mL),a concentration of 0.05 - 0.07 μg/mL of this antibody is required. Immunohistochemistry: The antibody stained formalin-fixed, paraffin embedded sections of normal human skin at1.0 μg/mL overnight at 4C. (A HRP-labeled polymer detection system was used with anon-alcohol soluble AEC chromogen and a proteinase K antigen retrieval). ELISA: Indirect - To detect hPDGF-AA by indirect ELISA (using 100 μL/well antibody solution),a concentration of 0.5 - 2.0 μg/mL of this antibody is required, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hPDGF-AA. Sandwich - To detect hPDGF-AA by sandwich ELISA (using 100 μL/well antibody solution),a concentration of 0.5 - 2.0 μg/mL of this antibody is required, in conjunction with abiotinylated anti-human PDGF-AA (PP1061B) as the detection antibody at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA can be detected. Western blot: To detect hPDGF-AA by Western blot analysis this antibody can be used at a concentrationof 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hPDGF-AA is 1.5 -3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PDGFA Antibody Target Details PDGFA Application Details Images back to top
Reconstitution Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2, without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody can be stored for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.
Expiry Date 12 months