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anti-Human PDGFA antibody for Functional Studies

Recommended PDGFA Antibody (supplied by: Log in to see )

Antigen
Platelet-Derived Growth Factor alpha Polypeptide (PDGFA) Antibodies
  • pdgfa
  • pdgfa-2
  • zgc:100777
  • pdgf
  • pdgf1
  • pdgf-a
  • PDGF-A
  • PDGF1
  • PDGFACP
  • platelet-derived growth factor alpha polypeptide
  • platelet-derived growth factor alpha a
  • collagen alpha-2(I) chain-like
  • platelet derived growth factor, alpha
  • Platelet-derived growth factor subunit A-like
  • platelet-derived growth factor subunit A-like
  • PDGFA
  • pdgfaa
  • pdgfa
  • LOC100735371
  • Pdgfa
  • LOC100352388
  • LOC100519764
Reactivity
Human
71
43
42
2
1
1
1
1
1
Host
Rabbit
64
6
3
2
Clonality
Polyclonal
Conjugate
This PDGFA antibody is un-conjugated
6
4
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
45
26
23
21
15
3
3
3
2
2
2
2
1
1
Supplier
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Catalog No. ABIN116208
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN116207 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2476090 Func IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 2
1 ABIN2476092 Func IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 2

Top referenced anti-PDGFA antibody for Functional Studies

Similar anti-PDGFA Antibodies

Application / Reactivity Human
Dot Blot (DB) 1 Antibodies
ELISA 19 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Functional Studies (Func) 4 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 21 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 43 Antibodies

General

Antigen Platelet-Derived Growth Factor alpha Polypeptide (PDGFA) Antibodies
Reactivity Human
(71), (43), (42), (2), (1), (1), (1), (1), (1)
Host Rabbit
(64), (6), (3), (2)
Clonality
Polyclonal
Conjugate This PDGFA antibody is un-conjugated
(6), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(45), (26), (23), (21), (15), (3), (3), (3), (2), (2), (2), (2), (1), (1)
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Product Details anti-PDGFA Antibody

Target Details PDGFA Application Details Handling Images
Specificity Human Platelet Derived Growth Factor AA (hPDGF-AA).
Purification Affinity Chromatography using an immobilized Human PDGF-AA matrix.
Immunogen Highly pure (>98%) E.coli derived recombinant Human PDGF-AA (Cat.-No PA118).

Target Details PDGFA

Product Details anti-PDGFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name PDGFA (PDGFA Antibody Abstract)
Background PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, PDGF AB and PDGF BB. The three isoforms bind with different affinities to two receptor types, A and B, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers, the A subunit of PDGF binds only to A receptors with high affinity, whereas the B subunit can bind to both A and B receptors. Evidence suggests that PDGF may function as a neurotrophic factor. The fact that PDGF-A receptors are expressed in oligodendrocyte progenitor cells, whereas PDGF-B receptors are expressed on neurons, suggests that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system by paracrine and autocrine routes.Synonyms: PDGF A, PDGF alpha, PDGF subunit A, PDGF-1, PDGF-A, PDGF1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha, Platelet-derived growth factor subunit A
Gene ID 5154
NCBI Accession NP_002598
UniProt P04085
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling

Application Details

Product Details anti-PDGFA Antibody Target Details PDGFA Handling Images back to top
Application Notes Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity ofhPDGF-AA (3.0 ng/mL), a concentration of 0.03 - 0.05 μg/mL of this antibody is required. Immunohistochemistry: A starting dilution of 1 μg/mL is recommended for frozen sections. ELISA: To detect hPDGF-AA by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 g/ ml of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hPDGF-AA. Western Blot: To detect hPDGF-AA by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.5 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPDGF-AA is 1.5 -3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PDGFA Antibody Target Details PDGFA Application Details Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.