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anti-Human PDGFA antibody for Western Blotting

Recommended PDGFA Antibody (supplied by: Log in to see )

Antigen
Platelet-Derived Growth Factor alpha Polypeptide (PDGFA) Antibodies
  • pdgf
  • PDGF-A
  • pdgf-a
  • PDGF1
  • pdgf1
  • pdgfa
  • pdgfa-2
  • PDGFACP
  • zgc:100777
Epitope
AA 60-89, N-Term
15
13
6
4
3
2
2
1
1
1
1
1
1
1
Reactivity
Human
88
38
37
2
1
1
1
Host
Rabbit
78
6
5
3
Clonality
Polyclonal
Conjugate
This PDGFA antibody is un-conjugated
7
5
4
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
65
35
31
22
16
6
4
4
3
2
2
2
2
1
1
Supplier
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Catalog No. ABIN388459
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12445667 ABIN726155 IF (p) IHC (p) WB Rabbit IgG AA 130-180 Log in to see Polyclonal 1
0.12445667 ABIN2774382 WB Rabbit C-Term Log in to see Polyclonal
0.12445667 ABIN2706781 IHC WB Rabbit Center Log in to see Polyclonal
0.12445667 ABIN498533 IHC (p) WB Rabbit Log in to see Polyclonal
0.12445667 ABIN1173032 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 87-211 Log in to see Polyclonal
0.12445667 ABIN2493021 IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.12445667 ABIN3186361 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
0.12445667 ABIN518736 IP WB Rabbit AA 1-211, full length Log in to see Polyclonal
0.12445667 ABIN446973 IHC (p) IHC WB Rabbit Lys154 Log in to see Polyclonal
0.12445667 ABIN518735 WB Mouse AA 1-211, full length Log in to see Polyclonal
0.12445667 ABIN518737 PLA WB Rabbit AA 1-211, full length Log in to see Polyclonal
0.0011801715 ABIN258831 IHC (p) IHC WB Mouse IgG2b Log in to see MM0013-6D35
0.0011801715 ABIN116206 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0011801715 ABIN116205 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0011801715 ABIN1103534 IHC (p) Neut ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011801715 ABIN1585666 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0011801715 ABIN116207 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
0.0011801715 ABIN116208 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
0.0011801715 ABIN1922542 IHC ELISA WB FITC Rabbit IgG N-Term Log in to see Polyclonal
0.0011801715 ABIN1922541 IHC ELISA WB APC Rabbit IgG N-Term Log in to see Polyclonal

Top referenced anti-PDGFA antibody for Western Blotting

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Application / Reactivity Human
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Functional Studies (Func) 6 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 1 Antibodies
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Immunohistochemistry (IHC) 31 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
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General

Antigen Platelet-Derived Growth Factor alpha Polypeptide (PDGFA) Antibodies
Epitope AA 60-89, N-Term
(15), (13), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(88), (38), (37), (2), (1), (1), (1)
Host Rabbit
(78), (6), (5), (3)
Clonality (Clone)
Polyclonal   ( )
Conjugate This PDGFA antibody is un-conjugated
(7), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(65), (35), (31), (22), (16), (6), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-PDGFA Antibody

Target Details PDGFA Application Details Handling References for anti-PDGFA Antibody (ABIN388459) Images
Specificity This PDGFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from amino acids 60-90 of human PDGFA.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This PDGFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 AA from the N-terminal region of human PDGFA.
Clone RB01999
Isotype Ig

Target Details PDGFA

Product Details anti-PDGFA Antibody Application Details Handling References for anti-PDGFA Antibody (ABIN388459) Images back to top
Antigen
Alternative Name PDGFA (PDGFA Antibody Abstract)
Background PDGFA is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis, knockout mice die either as embryos or shortly after birth.
Molecular Weight 24043 DA
Gene ID 5154
NCBI Accession NP_002598
Research Area Cell Signaling, Stem Cells, Cell Cycle, Cell Structure
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PDGFA Antibody Target Details PDGFA Handling References for anti-PDGFA Antibody (ABIN388459) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-PDGFA Antibody Target Details PDGFA Application Details References for anti-PDGFA Antibody (ABIN388459) Images back to top
Format Liquid
Concentration 1.03 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-PDGFA Antibody (ABIN388459)

Product Details anti-PDGFA Antibody Target Details PDGFA Application Details Handling Images back to top
Background publications

Müller, Richter, Rinas: "Kinetics control preferential heterodimer formation of platelet-derived growth factor from unfolded A- and B-chains." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 18330-5, 2003

Images

Product Details anti-PDGFA Antibody Target Details PDGFA Application Details Handling References for anti-PDGFA Antibody (ABIN388459) back to top
Supplier Images
Western Blotting (WB) image for anti-PDGFA antibody (Platelet-Derived Growth Factor alpha Polypeptide) (AA 60-89) (ABIN388459) The anti-PDGFA N-term Pab (ABIN388459) is used in Western blot to detect PDGFA in HL6...
Immunohistochemistry (IHC) image for anti-PDGFA antibody (Platelet-Derived Growth Factor alpha Polypeptide) (AA 60-89) (ABIN388459) Formalin-fixed and paraffin-embedded human brain tissue reacted with PDGFA antibody (...