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|Application / Reactivity||Human||Rat (Rattus)||Mouse (Murine)|
|Western Blotting (WB)||65 Antibodies||15 Antibodies||16 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Neutralization (Neut)||2 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||18 Antibodies||9 Antibodies||8 Antibodies|
|Immunohistochemistry (IHC)||31 Antibodies||9 Antibodies||9 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||23 Antibodies||23 Antibodies||23 Antibodies|
|Immunofluorescence (IF)||1 Antibodies|
|Antigen||Platelet-Derived Growth Factor alpha Polypeptide (PDGFA) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PDGFA antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-PDGFA AntibodyTarget Details PDGFA Application Details Handling Images
|Specificity||This detects endogenous levels of PDGF-A protein. (region surrounding Lys154)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % (by SDS-PAGE)|
Target Details PDGFAProduct Details anti-PDGFA Antibody Application Details Handling Images back to top
|Alternative Name||PDGFA (PDGFA Antibody Abstract)|
|Background||PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF-AA, PDGF-AB and PDGF-BB. The three isoforms bind with different affinities to two receptor types, α and β, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers, the A subunit of PDGF binds only to α receptors with high affinity, whereas the B subunit can bind to both α and β receptors. Evidence suggests that PDGF may function as a neurotrophic factor. The fact that receptors for PDGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons suggests that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system by paracrine and autocrine routes.Synonyms: PDGF A, PDGF alpha, PDGF subunit A, PDGF-1, PDGF-A, PDGF1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha, Platelet-derived growth factor subunit A|
|Molecular Weight||approx. 21 kDa|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-PDGFA Antibody Target Details PDGFA Handling Images back to top
Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PDGFA Antibody Target Details PDGFA Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-PDGFA Antibody Target Details PDGFA Application Details Handling back to top