anti-Human PDGFB antibody for Functional Studies

Recommended PDGFB Antibody (supplied by: Log in to see )

Antigen
Platelet-Derived Growth Factor beta Polypeptide (PDGFB) Antibodies
  • PDGF-2
  • PDGF2
  • SIS
  • SSV
  • c-sis
  • pdgf2
  • sis
  • si:ch211-79m20.1
  • si:dkey-261d11.1
  • PDGF-B
  • Sis
  • platelet-derived growth factor beta polypeptide
  • platelet derived growth factor, B polypeptide
  • PDGFB
  • pdgfb
  • Pdgfb
Reactivity
Human
105
47
46
3
3
3
1
Host
Rabbit
82
22
5
3
Clonality
Polyclonal
Conjugate
This PDGFB antibody is un-conjugated
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
84
43
31
27
16
13
6
5
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN116211
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN116212 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal 1

General

Antigen Platelet-Derived Growth Factor beta Polypeptide (PDGFB) Antibodies
Reactivity Human
(105), (47), (46), (3), (3), (3), (1)
Host Rabbit
(82), (22), (5), (3)
Clonality
Polyclonal
Conjugate This PDGFB antibody is un-conjugated
(6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(84), (43), (31), (27), (16), (13), (6), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PDGFB Antibody

Target Details PDGFB Application Details Handling References for anti-PDGFB antibody (ABIN116211) Images
Specificity Recognizes Human PDGF-BB.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography employing immobilized Human PDGF-BB matrix.
Immunogen Highly pure (>98%) E.coli derived recombinant Human PDGF-BB.

Target Details PDGFB

Product Details anti-PDGFB Antibody Application Details Handling References for anti-PDGFB antibody (ABIN116211) Images back to top
Antigen
Alternative Name PDGFB (PDGFB Antibody Abstract)
Background Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B, the dimers being connected by disulfide bonds. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.Synonyms: Becaplermin, PDGF B, PDGF beta, PDGF subunit B, PDGF-2, PDGF-B, PDGF2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta, Platelet-derived growth factor subunit B, SIS, c-Sis
Gene ID 5155
NCBI Accession NP_002599
UniProt P01127
Research Area Cytokines, Receptors, Inflammation
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling

Application Details

Product Details anti-PDGFB Antibody Target Details PDGFB Handling References for anti-PDGFB antibody (ABIN116211) Images back to top
Application Notes Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman PDGF-BB (2.5 ng/mL), a concentration of 0.03-0.04 μg/mL of this antibody isrequired. ELISAIndirect: To detect Human PDGF-BB by indirect ELISA (using 100 μL/well antibody solution)a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Human PDGF-BB. Sandwich: To detect Human PDGF-BB by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Human PDGF-BB (PP1062B1 orPP1062B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human PDGF-BB. Western Blot: To detect Human PDGF-BB by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human PDGF-BB is 1.5-3.0 ng/lane, undereither reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-PDGFB Antibody Target Details PDGFB Application Details References for anti-PDGFB antibody (ABIN116211) Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.
Expiry Date 12 months

References for anti-PDGFB antibody (ABIN116211)

Product Details anti-PDGFB Antibody Target Details PDGFB Application Details Handling Images back to top
Background publications

Tan, Pal, Tan, Wong, Tam, Teo, Chong, Tan, Goh, Tang, Cheung, Tan: "Regulation of cell proliferation and migration by TAK1 via transcriptional control of von Hippel-Lindau tumor suppressor." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18047-58, 2009