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anti-Human PDGFBB antibody for Blocking Antibody

Recommended PDGFBB Antibody (supplied by: Log in to see )

Antigen
Platelet Derived Growth Factor BB (PDGFBB) Antibodies
  • c-sis
  • PDGF-2
  • PDGF2
  • SIS
  • SSV
Reactivity
Human
34
8
7
Host
Rabbit
43
3
1
Clonality
Polyclonal
Conjugate
This PDGFBB antibody is un-conjugated
6
4
1
1
1
1
Application
Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
31
30
10
8
5
4
4
2
1
1
Supplier
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Supplier Product No.
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Catalog No. ABIN1003016
$ 374.22
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN636707 Inhibition ELISA WB Rabbit Log in to see Polyclonal

Similar anti-PDGFBB Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 2 Antibodies
ELISA 23 Antibodies
Functional Studies (Func) 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunohistochemistry (IHC) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies
Neutralization (Neut) 8 Antibodies
Western Blotting (WB) 25 Antibodies

General

Antigen Platelet Derived Growth Factor BB (PDGFBB) Antibodies
Reactivity Human
(34), (8), (7)
Host Rabbit
(43), (3), (1)
Clonality
Polyclonal
Conjugate This PDGFBB antibody is un-conjugated
(6), (4), (1), (1), (1), (1)
Application Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
(31), (30), (10), (8), (5), (4), (4), (2), (1), (1)
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Product Details anti-PDGFBB Antibody

Target Details PDGFBB Application Details Handling Images
Purification Antigen affinity purified polyclol antibody
Sterility Sterile filtered
Immunogen recombint hPDGF-BB (human PDGF-BB)
Isotype IgG

Target Details PDGFBB

Product Details anti-PDGFBB Antibody Application Details Handling Images back to top
Antigen
Alternative Name Platelet-Derived Growth Factor BB (PDGFBB Antibody Abstract)
Background Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPDGF-BB (human PDGF-BB). Anti-Human PDGF-BB specific antibody was purified by affinity chromatography employing immobilized hPDGF-BB matrix.
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PDGFBB Antibody Target Details PDGFBB Handling Images back to top
Application Notes To yield one-half maximal inhibition [ND₅₀] of the biological activity of hPDGF-BB (2.5 ng/µl), a concentration of 0.03 - 0.04 µg/µl of this antibody is required. To detect hPDGF-BB by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-BB. To detect hPDGF-BB by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Human PDGF-BB (PK-AB815-63022B) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-BB. To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunihostochemistry: NA. To detect hPDGF-BB by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Human PDGF-BB (PK-AB815-63022B) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-BB. To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-PDGFBB Antibody Target Details PDGFBB Application Details Images back to top
Format Lyophilized
Concentration To yield one-half maximal inhibition [ND₅₀] of the biological activity of hPDGF-BB (2.5 ng/µl), a concentration of 0.03 - 0.04 µg/µl of this antibody is required.
Buffer A sterile-filtered antibody solution was lyophilized from PBS, pH 7.2.
Handling Advice The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening. Reconstitute in sterile water at a concentration of 0.1-1.0 mg/ml.
Storage -20 °C
Expiry Date 24 months