anti-Human PDGFBB antibody for Neutralization

Recommended PDGFBB Antibody (supplied by: Log in to see )

Antigen
Platelet Derived Growth Factor BB (PDGFBB) Antibodies
  • PDGF-2
  • PDGF2
  • SIS
  • SSV
  • c-sis
  • platelet-derived growth factor beta polypeptide
  • PDGFB
Reactivity
Human
54
17
16
3
3
1
1
1
1
1
Host
Rabbit
62
4
2
1
Clonality
Polyclonal
Conjugate
This PDGFBB antibody is un-conjugated
8
5
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), ELISA, Western Blotting (WB)
64
44
18
8
6
5
3
3
3
2
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN465589
$ 493.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1982271 Neut WB Sheep IgG AA 82-190 Log in to see Polyclonal
1 ABIN2337063 Neut IHC ELISA WB APC Rabbit IgG Log in to see Polyclonal
1 ABIN2337053 Neut IHC ELISA WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN2337054 Neut IHC ELISA WB PE Rabbit IgG Log in to see Polyclonal
1 ABIN2337064 Neut IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN2337052 Neut IHC ELISA WB FITC Rabbit IgG Log in to see Polyclonal
1 ABIN2337062 Neut IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal
1 ABIN2337061 Neut IHC ELISA WB Rabbit IgG Log in to see Polyclonal

Similar anti-PDGFBB Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 2 Antibodies
ELISA 35 Antibodies
Functional Studies (Func) 3 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunohistochemistry (IHC) 12 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Neutralization (Neut) 9 Antibodies
Western Blotting (WB) 50 Antibodies

General

Antigen Platelet Derived Growth Factor BB (PDGFBB) Antibodies
Reactivity Human
(54), (17), (16), (3), (3), (1), (1), (1), (1), (1)
Host Rabbit
(62), (4), (2), (1)
Clonality
Polyclonal
Conjugate This PDGFBB antibody is un-conjugated
(8), (5), (2), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), ELISA, Western Blotting (WB)
(64), (44), (18), (8), (6), (5), (3), (3), (3), (2), (2), (1), (1)
Supplier Log in to see

Product Details anti-PDGFBB Antibody

Target Details PDGFBB Application Details Handling Images
Specificity Human PDGF-BB
Purification Immunoaffinity purified
Immunogen E.coli derived recombinant Human PDGF-BB.

Type of Immunogen: Cell extract

Target Details PDGFBB

Product Details anti-PDGFBB Antibody Application Details Handling Images back to top
Antigen
Alternative Name PDGF-BB (PDGFBB Antibody Abstract)
Background Name/Gene ID: PDGFB
Family: PDGF

Synonyms: PDGFB, Becaplermin, PDGF-BB, PDGF-b, SSV, PDGF subunit B, PDGF-2, PDGF2, Proto-oncogene c-Sis, SIS, C-sis, PDGF, B chain
Gene ID 5155
UniProt P01127
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling

Application Details

Product Details anti-PDGFBB Antibody Target Details PDGFBB Handling Images back to top
Application Notes Approved: ELISA, IHC-P (1 μg/mL), Neut (3 - 4 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hPDGF-BB (2.5 ng/mL), a concentration of 0.03-0.04 μg/mL of this antibody is required. ELISA: To detect hPDGF-BB by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PDGF-BB (LS-C104638) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-BB. Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-PDGFBB Antibody Target Details PDGFBB Application Details Images back to top
Format Lyophilized
Reconstitution Sterile water to a concentration of 0.1-1.0 mg/mL
Concentration Lot specific
Buffer Lyophilized from PBS, pH 7.2
Handling Advice Avoid freeze/thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Images

Product Details anti-PDGFBB Antibody Target Details PDGFBB Application Details Handling back to top
Supplier Images
 image for anti-PDGFBB antibody (Platelet Derived Growth Factor BB) (ABIN465589) anti-Platelet Derived Growth Factor BB (PDGFBB) antibody
 image for anti-PDGFBB antibody (Platelet Derived Growth Factor BB) (ABIN465589) anti-Platelet Derived Growth Factor BB (PDGFBB) antibody (Image 2)
 image for anti-PDGFBB antibody (Platelet Derived Growth Factor BB) (ABIN465589) anti-Platelet Derived Growth Factor BB (PDGFBB) antibody (Image 3)
 image for anti-PDGFBB antibody (Platelet Derived Growth Factor BB) (ABIN465589) anti-Platelet Derived Growth Factor BB (PDGFBB) antibody (Image 4)
 image for anti-PDGFBB antibody (Platelet Derived Growth Factor BB) (ABIN465589) anti-Platelet Derived Growth Factor BB (PDGFBB) antibody (Image 5)