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Dog (Canine) PDGFBB ELISA Kit

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Antigen
Platelet Derived Growth Factor BB (PDGFBB) ELISA Kits
  • c-sis
  • PDGF-2
  • PDGF2
  • SIS
  • SSV
Reactivity
Dog (Canine), Human
Alternatives
Kits with alternative reactivity to:
19
7
7
5
4
3
3
3
2
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
4-1.200 pg/mL
Minimum Detection Limit
4 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN2703403
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.0012584153 ABIN3032789 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4.9-1200 pg/mL 4.9 pg/mL Log in to see
0.0012584153 ABIN994747 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate 0.5-10 ng/mL 0.5 ng/mL Log in to see
0.0012584153 ABIN994789 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate 100-2500 pg/mL 100 pg/mL Log in to see

Similar PDGFBB ELISA Kits

Application / Reactivity Human Dog (Canine)
ELISA 20 ELISA Kits 4 ELISA Kits

General

Antigen Platelet Derived Growth Factor BB (PDGFBB) ELISA Kits
Reactivity Dog (Canine), Human
Kits with alternative reactivity to:
(19), (7), (7), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 4-1.200 pg/mL
Minimum Detection Limit 4 pg/mL
Application ELISA
Supplier Log in to see

Product Details PDGFBB ELISA Kit

Target details Application Details Handling Images
Purpose Canine PDGF-BB ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Cell Culture Supernatant, Plasma, Serum
Analytical Method Quantitative
Specificity This ELISA antibody pair detects canine PDGF-BB, it also can recognize human PDGF-BB.
Sensitivity 4 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details PDGFBB ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name PDGF-BB (PDGFBB ELISA Kit Abstract)
Background Gene Names: PDGFB
Protein names: Platelet-derived growth factor subunit B (PDGF subunit B) (PDGF-2) (Platelet-derived growth factor B chain) (Platelet-derived growth factor beta polypeptide)
Gene ID 442986
UniProt Q6Q7I7
Research Area Growth Factors
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details PDGFBB ELISA Kit Target details Handling Images back to top
Application Notes Recommended Dilution for serum and plasma samples20 fold
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details PDGFBB ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

Images

Product Details PDGFBB ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for PDGFBB ELISA Kit (Platelet Derived Growth Factor BB) (ABIN2703403) Platelet Derived Growth Factor BB (PDGFBB) ELISA Kit