You are viewing an incomplete version of our website. Please click to reload the website as full version.

Human PDGFBB ELISA Kit

Recommended PDGFBB Product (supplied by: Log in to see )

Antigen
Platelet Derived Growth Factor BB (PDGFBB) ELISA Kits
  • c-sis
  • PDGF-2
  • PDGF2
  • SIS
  • SSV
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
20
9
9
7
6
6
5
4
2
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
40.9-10000 pg/mL
Minimum Detection Limit
40.9 pg/mL
Application
ELISA
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2506951
$ 433.40
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.306289 ABIN924791 Sandwich ELISA Cell Lysate, Serum, Plasma 62-2000 pg/mL 62 pg/mL Log in to see
0.306289 ABIN414900 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012155632 ABIN3032979 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.55-400 pg/mL 0.55 pg/mL Log in to see
0.0012155632 ABIN3198604 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012155632 ABIN511484 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Log in to see
0.0012155632 ABIN625073 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 24
0.0012155632 ABIN771087 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids Log in to see
0.0012155632 ABIN1029590 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012155632 ABIN366843 Sandwich ELISA Serum, Plasma, Tissue Homogenate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155632 ABIN2703403 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4-1.200 pg/mL 4 pg/mL Log in to see
0.0012155632 ABIN625074 Sandwich ELISA Tissue Lysate, Cell Lysate 1-400 pg/mL 1 pg/mL Log in to see
0.0012155632 ABIN2114770 Cell Lysate, Serum, Plasma 62-2000 pg/mL 62 pg/mL Log in to see
0.0012155632 ABIN2703402 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 10-2.500 pg/mL 10 pg/mL Log in to see
0.0012155632 ABIN2748523 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids 8-2000 pg/mL Log in to see
0.0012155632 ABIN2537658 Tissue Homogenate, Biological Fluids 4.6-300 pg/mL 4.6 pg/mL Log in to see
0.0012155632 ABIN2537968 Sandwich ELISA Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012155632 ABIN3014786 Sandwich ELISA Log in to see
0.0012155632 ABIN2950461 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012155632 ABIN2101410 Sandwich ELISA Log in to see

Top referenced PDGFBB ELISA Kit for ELISA

  • Human PDGFBB ELISA Kit for Sandwich ELISA - ABIN625073 (24 Publications): Miyahara, Nyan, Shimoda, Yamamoto, Kuroda, Shiota, Akiyoshi, Kasugai: Exploitation of a novel polysaccharide nanogel cross-linking membrane for guided bone regeneration (GBR). in Journal of tissue engineering and regenerative medicine 2011 (PubMed)

Similar PDGFBB ELISA Kits

Application / Reactivity Human
ELISA 20 ELISA Kits

General

Antigen Platelet Derived Growth Factor BB (PDGFBB) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(20), (9), (9), (7), (6), (6), (5), (4), (2), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 40.9-10000 pg/mL
Minimum Detection Limit 40.9 pg/mL
Application ELISA
Supplier Log in to see

Product Details PDGFBB ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PDGF-BB in human serum, plasma, and other biological fluids not being tested
Brand EasyTest™
Sample Type Serum, Plasma
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of human PDGF-BB
Sensitivity <1 pg/mL
Characteristics one wash
Components
  • Assay plate (12x8 coated Microwells)-1
  • Standard-2x 10μL
  • human PDGF-BB Mix-4x 9 μL
  • Assay diluent (5x concentrate)-1 x15mL
  • Wash buffer (20x concentrate)-1x25 mL
  • TMB substrate-1x12mL
  • Stop solution-1x 8mL
  • Adhensive strip-2
  • Instruction manual-1.
Material not included 1. Distilled or deionized water, 2.Precision pipettes, with disposable plastic tips, 3.Beakers, flasks, cylinders necessary for preparation of reagents, 4.Microplate washing device (multichannel pipette or automated microplate washer), 5.Microplate shaker, 6.Microplate reader capable of reading at 450 nm.

Target details

Product Details PDGFBB ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name PDGF-BB (PDGFBB ELISA Kit Abstract)
Background Human PDGF (Platelet-Derived Growth Factor) is a general term for a small group of structurally related and secreted growth factors.PDGF-BB is expressed by hepatocytes and nonresorbing osteoclasts, generating osteoblasts and bone formation. It is also produced by platelets, macrophages, and mast cells, and at sites of injury, promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new ECM, and IFG-I mediated re-epithelialization.
Molecular Weight 25 kDa
Gene ID 5155
NCBI Accession NP_002599
UniProt P01127
Research Area Growth Factors
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details PDGFBB ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Sample Volume 50 μL
Assay Time 1.5 h
Plate Strips (12 x 8)
Protocol This assay employs the fast quantitative sandwich enzyme immunoassay technique. Antibody specific for PDGF-BB has been pre-coated onto a microplate. Standards, samples and human PDGF-BB mix are pipetted into the wells and any PDGF-BB present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Reagent Preparation 1. Assay diluent: Dilute the concentrated assay diluent 1:5 with distilled water (e.g. 10 mL plus 40 mL). 2. Wash buffer: Dilute the concentrated wash buffer 1:20 with distilled water (e.g. 20 mL plus 380 mL). 3. Standard: Briefly spin standard vial before use. Add 390 μL 1x Assay Diluent to prepare a 50 ng/mL standard. Gently vortex to mix.Take 100 μL PDGF-BB standard into a tube, then add 400 μL 1x Assay Diluent to prepare a 10000 pg/mL stock standard solution.Add 150 μL 1x Assay Diluent to 7 tubes. Perform serial dilutions by adding 100 μL of each standard to the next tube and vortexing between each transfer, the last tube with 1x assay diluent is the blank as 0pg/mL. 4. human PDGF-BB Mix: Within 15 minutes prior to use, briefly spin the vial. Add 1490 μL of 1x Assay diluent to the vial and mix by pipetting. A vial mix can be used for about 30 wells.
Sample Collection Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 °C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or citrate or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 °C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Sample Preparation Levels of the target protein may vary among different specimens. Optimal dilution factors for each sample must be determined by the investigator,The dilution scheme is only suggestion: the recommended dilution for serum and plasma is 1:5.
Assay Procedure 1. All reagents must be brought to room temperature (18-25 °C) prior to use 2. Prepare all reagents, working standards,samples and mix as directed in the respective sections. 3.Determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4 °C. 4. Add 50μL of standard or sample to the designated wells, Gentlely shake/tap the plate for 5 second to mix. 5. Add 50 μL of PDGF-BB Mix into all wells, including the blank wells, cover the plate with adhensive strip. Incubate for 1 hour at room temperature with gentle shaking. 6.Decant or aspirate contents of wells. Wash wells by filling with at least 300 μL/well prepared wash buffer followed by decanting/aspirating. Soak wells in wash buffer for 30 seconds to 1 minute for each wash. Repeat wash 4 times for a total of 5 washes. After the last wash, blot plate on absorbent paper to remove residual buffer. Thorough washing at this step is very important, complete removal of liquid is required for proper performance. 7.Pipette 100 μL of TMB Substrate Solution to each well. Incubate plate for 15 minutes at room temperature in the dark with gentle shaking. 8. Add 50μL of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 9. Determine the optical density of each well within 15 minutes using a microplate reader set to 450nm. If wavelength correction is available, set to 570nm. Subtract readings at 570nm from the readings at 450nm. This subtraction will correct for optical imperfections in the plate.
Calculation of Results 1. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.2. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the target antigen concentration versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. 3. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
Assay Precision intra CV<10%, inter CV <15%
Restrictions For Research Use only

Handling

Product Details PDGFBB ELISA Kit Target details Application Details Images back to top
Buffer 0.05 % Proclin 300
Preservative ProClin
Precaution of Use The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
Storage 4 °C/-20 °C

Images

Product Details PDGFBB ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for PDGFBB ELISA Kit (Platelet Derived Growth Factor BB) (ABIN2506951) Platelet Derived Growth Factor BB (PDGFBB) ELISA Kit