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Pig (Porcine) PDGFBB ELISA Kit

Recommended PDGFBB Product (supplied by: Log in to see )

Antigen
Platelet Derived Growth Factor BB (PDGFBB) ELISA Kits
  • PDGF-2
  • PDGF2
  • SIS
  • SSV
  • c-sis
  • platelet-derived growth factor beta polypeptide
  • PDGFB
Reactivity
Human, Pig (Porcine)
Alternatives
Kits with alternative reactivity to:
23
10
9
7
5
4
3
3
2
1
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
10-2.500 pg/mL
Minimum Detection Limit
10 pg/mL
Application
ELISA
Options
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Catalog No. ABIN2703402
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN3032859 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 10.2-2500 pg/mL 10.2 pg/mL Log in to see
1 ABIN1565531 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
1 ABIN779521 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids Log in to see

Similar PDGFBB ELISA Kits

Application / Reactivity Human Pig (Porcine)
ELISA 24 ELISA Kits 4 ELISA Kits

General

Antigen Platelet Derived Growth Factor BB (PDGFBB) ELISA Kits
Reactivity Human, Pig (Porcine)
Kits with alternative reactivity to:
(23), (10), (9), (7), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 10-2.500 pg/mL
Minimum Detection Limit 10 pg/mL
Application ELISA
Supplier Log in to see

Product Details PDGFBB ELISA Kit

Target details Application Details Handling Images
Purpose Porcine PDGF-BB ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Cell Culture Supernatant, Plasma, Serum
Analytical Method Quantitative
Specificity This ELISA antibody pair detects porcine and human PDGF-BB. Other species not determined.
Sensitivity 10 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details PDGFBB ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name PDGF-BB (PDGFBB ELISA Kit Abstract)
Background Gene Names: PDGFB
Protein names: Platelet-derived growth factor subunit B (PDGF subunit B) (PDGF-2) (Platelet-derived growth factor B chain) (Platelet-derived growth factor beta polypeptide) (Fragment)
UniProt P20034
Research Area Growth Factors
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling

Application Details

Product Details PDGFBB ELISA Kit Target details Handling Images back to top
Application Notes Recommended Dilution for serum and plasma samples2-20 fold
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details PDGFBB ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

Images

Product Details PDGFBB ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for PDGFBB ELISA Kit (Platelet Derived Growth Factor BB) (ABIN2703402) Platelet Derived Growth Factor BB (PDGFBB) ELISA Kit