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|Application / Reactivity||Human|
|Dot Blot (DB)||14 Antibodies|
|Enzyme Immunoassay (EIA)||9 Antibodies|
|Flow Cytometry (FACS)||36 Antibodies|
|Functional Studies (Func)||3 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||20 Antibodies|
|Immunofluorescence (IF)||36 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||59 Antibodies|
|Immunohistochemistry (IHC)||140 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||73 Antibodies|
|Immunoprecipitation (IP)||10 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Proximity Ligation Assay (PLA)||3 Antibodies|
|Antigen||Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) Antibodies|
|Conjugate||This PDGFRA antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|5 references available|
|Supplier||Log in to see|
Product Details anti-PDGFRA AntibodyTarget Details PDGFRA Application Details Handling References for anti-PDGFRA Antibody (ABIN359906) Images
|Specificity||This antibody reacts to PDGFRA.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PDGFRA.|
Target Details PDGFRAProduct Details anti-PDGFRA Antibody Application Details Handling References for anti-PDGFRA Antibody (ABIN359906) Images back to top
|Alternative Name||CD140a / PDGFRA (PDGFRA Antibody Abstract)|
|Background||PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.Synonyms: Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, PDGF Receptor alpha, PDGF-R-alpha|
|Gene ID||5156, 9606|
|Research Area||CD Antigens, Surface Receptors of Immune Cells, Growth Factors|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-PDGFRA Antibody Target Details PDGFRA Handling References for anti-PDGFRA Antibody (ABIN359906) Images back to top
ELISA: 1/1,000. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PDGFRA Antibody Target Details PDGFRA Application Details References for anti-PDGFRA Antibody (ABIN359906) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-PDGFRA Antibody (ABIN359906)Product Details anti-PDGFRA Antibody Target Details PDGFRA Application Details Handling Images back to top
Cools, DeAngelo, Gotlib et al.: "A tyrosine kinase created by fusion of the PDGFRA and FIP1L1 genes as a therapeutic target of imatinib in idiopathic hypereosinophilic syndrome. ..." in: The New England journal of medicine, Vol. 348, Issue 13, pp. 1201-14, 2003 (PubMed).
Heinrich, Corless, Duensing et al.: "PDGFRA activating mutations in gastrointestinal stromal tumors." in: Science (New York, N.Y.), Vol. 299, Issue 5607, pp. 708-10, 2003 (PubMed).
Andrae, Molander, Smits et al.: "Platelet-derived growth factor-B and -C and active alpha-receptors in medulloblastoma cells." in: Biochemical and biophysical research communications, Vol. 296, Issue 3, pp. 604-11, 2002 (PubMed).
Ribom, Andrae, Frielingsdorf et al.: "Prognostic value of platelet derived growth factor alpha receptor expression in grade 2 astrocytomas and oligoastrocytomas." in: Journal of neurology, neurosurgery, and psychiatry, Vol. 72, Issue 6, pp. 782-7, 2002 (PubMed).
Kawagishi, Kumabe, Yoshimoto et al.: "Structure, organization, and transcription units of the human alpha-platelet-derived growth factor receptor gene, PDGFRA." in: Genomics, Vol. 30, Issue 2, pp. 224-32, 1996 (PubMed).
ImagesProduct Details anti-PDGFRA Antibody Target Details PDGFRA Application Details Handling References for anti-PDGFRA Antibody (ABIN359906) back to top