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anti-Human PDGFRA antibody for Flow Cytometry

Recommended PDGFRA Antibody (supplied by: Log in to see )

Antigen
Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) Antibodies
  • AI115593
  • APDGFR
  • CD140A
  • CD140a
  • PDGFACE
  • PDGFR
  • PDGFR-2
  • Pdgfr-2
  • PDGFR-A
  • PDGFR2
  • RHEPDGFRA
Reactivity
Human
375
174
124
4
3
3
3
3
2
1
1
1
1
1
1
Host
Mouse
330
64
24
1
1
Clonality
Monoclonal
Conjugate
This PDGFRA antibody is conjugated to FITC
29
24
24
21
19
14
5
5
5
5
5
5
5
5
5
1
1
1
Application
Flow Cytometry (FACS)
248
173
143
70
57
56
35
21
14
10
9
5
4
3
1
1
1
1
Supplier
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Catalog No. ABIN492425
$ 346.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3068567 ABIN492424 FACS IF Mouse IgG1 Log in to see 16A1
0.3068567 ABIN2660947 FACS Biotin Mouse IgG1 kappa Log in to see 16A1 6
0.3068567 ABIN2663025 FACS PE Mouse IgG1 kappa Log in to see 16A1 7
0.3068567 ABIN2664221 FACS Mouse IgG1 kappa Log in to see 16A1 6
0.3068567 ABIN2493032 FACS IHC WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.0010666476 ABIN392022 FACS IHC (p) WB Rabbit Ig AA 1048-1077, C-Term Log in to see Polyclonal 1
0.0010666476 ABIN457314 FACS Biotin Mouse IgG1 Log in to see 16A1 4
0.0010666476 ABIN457411 ICC FACS Mouse IgG1 Log in to see 16A1 4
0.0010666476 ABIN452287 FACS Biotin Mouse IgG1 Log in to see 16A1
0.0010666476 ABIN457360 FACS PE Mouse IgG1 Log in to see 16A1 4
0.0010666476 ABIN452286 FACS APC Mouse IgG1 Log in to see 16A1
0.0010666476 ABIN325544 FACS IF IHC (p) IP WB Rabbit IgG C-Term Log in to see
0.0010666476 ABIN452289 FACS IF Mouse IgG1 Log in to see 16A1
0.0010666476 ABIN2660948 FACS Biotin Mouse IgG1 kappa Log in to see 16A1 6
0.0010666476 ABIN221937 FACS APC Mouse IgG1 Log in to see
0.0010666476 ABIN490304 FACS APC Mouse IgG1 Log in to see 16A1
0.0010666476 ABIN490305 FACS PE Mouse IgG1 Log in to see 16A1
0.0010666476 ABIN490303 FACS IF Biotin Mouse IgG1 Log in to see 16A1
0.0010666476 ABIN2663026 FACS PE Mouse IgG1 kappa Log in to see 16A1 7
0.0010666476 ABIN490145 ICC FACS Mouse IgG1 Log in to see 16A1

Top referenced anti-PDGFRA antibody for Flow Cytometry

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General

Antigen Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) Antibodies
Reactivity Human
(375), (174), (124), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(330), (64), (24), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PDGFRA antibody is conjugated to FITC
(29), (24), (24), (21), (19), (14), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1)
Application Flow Cytometry (FACS)
(248), (173), (143), (70), (57), (56), (35), (21), (14), (10), (9), (5), (4), (3), (1), (1), (1), (1)
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Product Details anti-PDGFRA Antibody

Target Details PDGFRA Application Details Handling Images
Specificity This antibody reacts with CD140a antigen on Flow Cytometry.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: PDGF-R-alpha, PDGF Receptor alpha, Alpha-type platelet-derived growth factor receptor,CD140 antigen-like family member A
Purification Protein-A Agarose Chromatography of hybridoma supernatant.
Immunogen Human CD140a transfected NIH/3T3.
Clone 16A1
Isotype IgG1

Target Details PDGFRA

Product Details anti-PDGFRA Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD140a / PDGFRA (PDGFRA Antibody Abstract)
Background The platelet-derived growth-factor (PDGF) family induces a variety of cellular responses including cell proliferation, migration, differentiation, and matrix deposition. The PDGF ligands are dimeric molecules encoded by three different genes, PDGF-A, -B, -C, and the recently described PDGF-D. Two tyrosine kinase receptors, PDGFR-alpha and PDGFR-beta, are also known CD140a and CD140b respectively, become activated upon ligand binding and dimerization. The binding of effector proteins to the activated PDGFR results in the activation of multiple signal transduction pathways. PDGFR-alpha binds PDGF-A, -B and -C, whereas PDGFR-beta binds PDGF-B and -D. The pleiotropic effect of PDGFs on various cell types and the expression of PDGFR-alpha in a variety of tissues throughout embryonic development and postnatal life suggest its roles in multiple processes such as gastrulation, vasculogenesis, wound healing, and glial cell development.Synonyms: Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, PDGF Receptor alpha, PDGF-R-alpha
Gene ID 5156
UniProt P16234
Research Area CD Antigens, Surface Receptors of Immune Cells, Growth Factors
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PDGFRA Antibody Target Details PDGFRA Handling Images back to top
Application Notes Flow Cytometry: 5-10 μg/mL (final concentration). Positive Control: Human Transfectant. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Flow cytometric analysis for adherent cellsWe usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Detach the cells from culture dish by trypsinization. *Excessive trypsinization mayreduce the antigenecity. 2) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS)and 0. 1% NaN]. 3) Resuspend the cells with washing buffer (5x106 cells/mL)4) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute atRT (20~25°C). Remove supernatant by careful aspiration. 5) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3to the cell pellet after tapping. Mix well and incubate for 5 minutes at RT. 6) Add 40 µL of the FITC labeled anti-PDGF receptor alpha/CD140a antibody (Clone 16A1)(12. 5-25 µg/mL) diluted with the washing buffer. Mix well and incubate for 30 minutes atRT. 7) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at RT. Remove supernatant by careful aspiration. 8) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Positive Control: Human Transfectant.
Restrictions For Research Use only

Handling

Product Details anti-PDGFRA Antibody Target Details PDGFRA Application Details Images back to top
Concentration 50 µg/mL
Buffer PBS containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-PDGFRA Antibody Target Details PDGFRA Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-PDGFRA antibody (Platelet-Derived Growth Factor Receptor, alpha Polypeptide)  (FITC) (ABIN492425) anti-Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) antibody (FITC)