anti-Human PDGFRA antibody for Functional Studies

Recommended PDGFRA Antibody (supplied by: Log in to see )

Antigen
Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) Antibodies
  • AI115593
  • CD140a
  • Pdgfr-2
  • APDGFR
  • PDGFACE
  • CD140A
  • PDGFR-2
  • PDGFR2
  • RHEPDGFRA
  • PDGFR
  • PDGFR-A
  • platelet derived growth factor receptor alpha
  • platelet derived growth factor receptor, alpha polypeptide
  • platelet-derived growth factor receptor, alpha polypeptide L homeolog
  • platelet-derived growth factor receptor, alpha polypeptide
  • PDGFRA
  • Pdgfra
  • pdgfra.L
Reactivity
Human
482
256
160
7
6
6
6
6
4
3
3
3
2
2
1
1
1
1
Host
Mouse
358
149
49
3
1
Clonality
Monoclonal
Conjugate
This PDGFRA antibody is un-conjugated
36
31
26
26
22
14
13
12
7
7
7
7
6
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
2
1
1
1
Application
Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
326
194
193
134
110
67
49
44
15
14
12
9
9
3
3
2
1
1
1
1
1
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Catalog No. ABIN260348
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2688972 Func FACS IP Mouse IgG2a, kappa Log in to see alphaR1 1
1 ABIN640562 Func IHC (p) ELISA WB Mouse Log in to see

General

Antigen Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) Antibodies
Reactivity Human
(482), (256), (160), (7), (6), (6), (6), (6), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(358), (149), (49), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PDGFRA antibody is un-conjugated
(36), (31), (26), (26), (22), (14), (13), (12), (7), (7), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (2), (1), (1), (1)
Application Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(326), (194), (193), (134), (110), (67), (49), (44), (15), (14), (12), (9), (9), (3), (3), (2), (1), (1), (1), (1), (1)
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Product Details anti-PDGFRA Antibody

Target Details PDGFRA Application Details Handling Images
Specificity This antibody is specific for PDGF receptor alpha. The ND50 was determined as 0.2-0.6 μg/mL for blocking 10 ng/mL of PDGF-AA biological activity on a quiescent confluent cell culture of human WS1 cells. PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cellsurface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA. Both PDGF receptor alpha and beta are members of the class III subfamily of receptor tyrosine kinases.
Purification Protein G purified
Immunogen Purified recombinant human platelet-derived growth factor (PDGF) receptor alpha (extracellular domain), derived from the insect cell line Sf21.
Clone 35248 (57-20)
Isotype IgG1

Target Details PDGFRA

Product Details anti-PDGFRA Antibody Application Details Handling Images back to top
Antigen
Alternative Name PDGF R alpha (PDGFRA Antibody Abstract)
Background Gene Symbol: PDGFRA
Gene ID 5156
UniProt P16234
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling

Application Details

Product Details anti-PDGFRA Antibody Target Details PDGFRA Handling Images back to top
Application Notes Western Blot 1 μg/mL-2 μg/mL, ELISA 0.5 μg/mL-1 μg/mL, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 10 μg/mL-20 μg/mL, Functional
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-PDGFRA Antibody Target Details PDGFRA Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.2) and 1.0 % Trehalose
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.