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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||9 Antibodies||3 Antibodies||3 Antibodies|
|Cytometry by Time of Flight (CyTOF)||2 Antibodies|
|Western Blotting (WB)||252 Antibodies||149 Antibodies||74 Antibodies|
|Proximity Ligation Assay (PLA)||3 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|In vitro Assay (in vitro)||1 Antibodies|
|Immunoprecipitation (IP)||12 Antibodies||5 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||82 Antibodies||60 Antibodies||40 Antibodies|
|Immunohistochemistry (IHC)||84 Antibodies||145 Antibodies|
|Antigen||Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PDGFRA antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-PDGFRA AntibodyTarget Details PDGFRA Application Details Handling Images
|Specificity||Specific for PDGFR, alpha and does not react with PDGFR, beta. Cellular Localization Cell membrane.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Protein A Chromatography.|
|Immunogen||A synthetic peptide derived from the C-terminus of the precursor form of Human PDGFR alpha.|
Target Details PDGFRAProduct Details anti-PDGFRA Antibody Application Details Handling Images back to top
|Alternative Name||CD140a / PDGFRA (PDGFRA Antibody Abstract)|
|Background||PDGFR (Platelet Derived Growth Factor Receptor) exhibits two different isoforms, alpha and beta coded by two different genes. They possess five immunoglobulin like domains that are involved in ligand binding. Two tyrosine kinase domains are separated by a kinase insert to which PI-3 kinase can bind. PDGF AA can bind autophosphorylate and activate only PDGFR, alpha while PDGF BB can bind and activate to both PDGFR, alpha and beta.Synonyms: Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, PDGF Receptor alpha, PDGF-R-alpha|
|Research Area||CD Antigens, Surface Receptors of Immune Cells, Growth Factors|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling|
Application DetailsProduct Details anti-PDGFRA Antibody Target Details PDGFRA Handling Images back to top
ELISA. Immunohistochemistry on Paraffin Sections: Use Ab at 5-10 μg/mL for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive Control: Ovariancarcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PDGFRA Antibody Target Details PDGFRA Application Details Images back to top
|Buffer||PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 0.2 % BSA as stabilizer, before the addition of Glycerol to 40 %.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
ImagesProduct Details anti-PDGFRA Antibody Target Details PDGFRA Application Details Handling back to top