anti-Mouse (Murine) PDGFRA antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended PDGFRA Antibody (supplied by: Log in to see )

Antigen
Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) Antibodies
  • AI115593
  • CD140a
  • Pdgfr-2
  • APDGFR
  • PDGFACE
  • CD140A
  • PDGFR-2
  • PDGFR2
  • RHEPDGFRA
  • PDGFR
  • PDGFR-A
  • platelet-derived growth factor receptor, alpha polypeptide
  • platelet derived growth factor receptor, alpha polypeptide
  • platelet-derived growth factor receptor alpha
  • PDGFRA
  • LOC100218386
  • Pdgfra
  • pdgfra
  • LOC100343006
Reactivity
Mouse (Murine)
479
255
159
7
6
6
6
6
4
3
3
3
2
2
1
1
1
1
Host
Rat
355
149
48
4
1
Clonality
Monoclonal
Conjugate
This PDGFRA antibody is un-conjugated
36
31
27
26
22
14
13
12
7
7
7
7
6
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
2
1
1
1
Application
Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
322
193
192
133
111
67
49
43
14
14
12
9
9
3
3
2
1
1
1
1
Options
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Catalog No. ABIN967626
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Top referenced anti-PDGFRA antibody for Immunohistochemistry (Formalin-fixed Sections)

Similar anti-PDGFRA Antibodies

Application / Reactivity Mouse (Murine)
ELISA 64 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 41 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 67 Antibodies
Immunohistochemistry (IHC) 84 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 15 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 60 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 149 Antibodies

General

Antigen Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) Antibodies
Reactivity Mouse (Murine)
(479), (255), (159), (7), (6), (6), (6), (6), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rat
(355), (149), (48), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PDGFRA antibody is un-conjugated
(36), (31), (27), (26), (22), (14), (13), (12), (7), (7), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (2), (1), (1), (1)
Application Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(322), (193), (192), (133), (111), (67), (49), (43), (14), (14), (12), (9), (9), (3), (3), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-PDGFRA Antibody

Target Details PDGFRA Application Details Handling ProductDetails: References for anti-PDGFRA antibody (ABIN967626) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse PDGF Receptor alpha chain
Clone APA5
Isotype IgG2a, kappa

Target Details PDGFRA

Product Details anti-PDGFRA Antibody Application Details Handling ProductDetails: References for anti-PDGFRA antibody (ABIN967626) Images back to top
Antigen
Alternative Name CD140a (PDGFRA Antibody Abstract)
Background The APA5 antibody reacts with Platelet-Derived Growth Factor (PDGF) Receptor alpha chain (CD140a, PDGFR-a), a receptor tyrosine kinase which is widely expressed on cells of mesenchymal origin in the embryo and adult and on several other cell types during embryonic development, but not on hematopoietic cells. PDGFR-a binds to PDGF A and B chains, in contrast to PDGFR-b, which binds only to the PDGF B chain. Biologically active PDGF is a disulphide-linked dimer, forming the AA, AB, and BB isoforms. Binding to PDGF Receptor induces the formation of receptor dimers (aa, ab, or bb), autophosphorylation, and internalization. The mAb APA5 has been demonstrated to block binding of PDGF-AA to PDGFR-a-expressing cells in vitro and to block some PDGF-mediated developmental events in vivo.
Synonyms: PDGF Receptor alpha chain
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling

Application Details

Product Details anti-PDGFRA Antibody Target Details PDGFRA Handling ProductDetails: References for anti-PDGFRA antibody (ABIN967626) Images back to top
Application Notes This antibody has been tested by immunofluorescent staining (Less or equal than 1 µg/million cells) with flow cytometric analysis and immunohistochemical staining of acetone-fixed frozen sections (0.5 - 2.5 µg/ml) to assure specificity and reactivity. Other reported applications include western blot analysis, ELISA, immunohistochemical staining of paraffin-embedded sections, inhibition of ligand binding, and in vivo blocking of PDGF action.
Restrictions For Research Use only

Handling

Product Details anti-PDGFRA Antibody Target Details PDGFRA Application Details ProductDetails: References for anti-PDGFRA antibody (ABIN967626) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

ProductDetails: References for anti-PDGFRA antibody (ABIN967626)

Product Details anti-PDGFRA Antibody Target Details PDGFRA Application Details Handling Images back to top
Product cited in:

Fruttiger, Calver, Krüger, Mudhar, Michalovich, Takakura, Nishikawa, Richardson: "PDGF mediates a neuron-astrocyte interaction in the developing retina." in: Neuron, Vol. 17, Issue 6, pp. 1117-31, 1997

Takakura, Yoshida, Kunisada, Nishikawa, Nishikawa: "Involvement of platelet-derived growth factor receptor-alpha in hair canal formation." in: The Journal of investigative dermatology, Vol. 107, Issue 5, pp. 770-7, 1996

Heldin: "Structural and functional studies on platelet-derived growth factor." in: The EMBO journal, Vol. 11, Issue 12, pp. 4251-9, 1992