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anti-Dog (Canine) Phospholipase C gamma 1 antibody for Immunofluorescence

Recommended Phospholipase C gamma 1 Antibody (supplied by: Log in to see )

Antigen
phospholipase C, gamma 1 (PLCG1) Antibodies
  • AI894140
  • Cded
  • nckap3
  • NCKAP3
  • Phosphb
  • PLC'1
  • Plc-1
  • Plc-gamma1
  • PLC-II
  • plc-ii
  • plc1
  • PLC1
  • plc148
  • PLC148
  • PLCbeta1
  • Plcg-1
  • plcgamma1
  • PLCgamma1
  • PPLCA
  • RATPHOSPHB
Epitope
AA 82-100
50
41
13
13
9
9
8
8
6
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
216
131
124
11
8
4
3
1
Host
Mouse
192
28
Clonality
Monoclonal
Conjugate
This Phospholipase C gamma 1 antibody is un-conjugated
5
5
4
4
3
3
3
3
3
3
3
3
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
157
56
49
34
30
27
25
6
4
4
2
1
1
1
1
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Catalog No. ABIN967699
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Top referenced anti-Phospholipase C gamma 1 antibody for Immunofluorescence

Similar anti-Phospholipase C gamma 1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Chicken
Western Blotting (WB) 155 Antibodies 82 Antibodies 76 Antibodies 3 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Intracellular Staining (ICS) 2 Antibodies 2 Antibodies 4 Antibodies 2 Antibodies 2 Antibodies
Immunoprecipitation (IP) 22 Antibodies 4 Antibodies 4 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies 16 Antibodies 16 Antibodies
Immunohistochemistry (IHC) 57 Antibodies

General

Antigen phospholipase C, gamma 1 (PLCG1) Antibodies
Epitope AA 82-100
(50), (41), (13), (13), (9), (9), (8), (8), (6), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(216), (131), (124), (11), (8), (4), (3), (1)
Host Mouse
(192), (28)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Phospholipase C gamma 1 antibody is un-conjugated
(5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(157), (56), (49), (34), (30), (27), (25), (6), (4), (4), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Phospholipase C gamma 1 Antibody

Target Details Phospholipase C gamma 1 Application Details Handling References for anti-Phospholipase C gamma 1 Antibody (ABIN967699) Images
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Cow PLCgamma1 aa. 82-100
Clone 10-PLCgamma
Isotype IgG1

Target Details Phospholipase C gamma 1

Product Details anti-Phospholipase C gamma 1 Antibody Application Details Handling References for anti-Phospholipase C gamma 1 Antibody (ABIN967699) Images back to top
Antigen
Alternative Name Phospholipase C gamma 1 (PLCG1 Antibody Abstract)
Background The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLCg is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLCg1, which is ubiquitously expressed, and 2) PLCg2, found primarily in the lymphoid system. PLCg is essential for growth factor-induced cell motility and mitogenesis. PLCg1 null mice exhibit retarded embryonic growth and lethality in midgestation. Overexpression of PLCg is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus regulation of PLCg activity by growth factors is involved in cell growth and transformation.
The 10/PLCgamma monoclonal antibody recognizes PLCg1, regardless of phosphorylation status. It does not cross-react with PLCg2.
Molecular Weight 148 kDa
Pathways RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Handling References for anti-Phospholipase C gamma 1 Antibody (ABIN967699) Images back to top
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details References for anti-Phospholipase C gamma 1 Antibody (ABIN967699) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-Phospholipase C gamma 1 Antibody (ABIN967699)

Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details Handling Images back to top
Product cited in:

Vossmeyer, Hofmann, Löster, Reutter, Danker: "Phospholipase Cgamma binds alpha1beta1 integrin and modulates alpha1beta1 integrin-specific adhesion." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4636-43, 2002

Dayanir, Meyer, Lashkari, Rahimi: "Identification of tyrosine residues in vascular endothelial growth factor receptor-2/FLK-1 involved in activation of phosphatidylinositol 3-kinase and cell proliferation." in: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 17686-92, 2001

Harder, Kuhn: "Selective accumulation of raft-associated membrane protein LAT in T cell receptor signaling assemblies." in: The Journal of cell biology, Vol. 151, Issue 2, pp. 199-208, 2000

Chen, Murphy-Ullrich, Wells: "A role for gelsolin in actuating epidermal growth factor receptor-mediated cell motility." in: The Journal of cell biology, Vol. 134, Issue 3, pp. 689-98, 1996

Obermeier, Tinhofer, Grunicke, Ullrich: "Transforming potentials of epidermal growth factor and nerve growth factor receptors inversely correlate with their phospholipase C gamma affinity and signal activation." in: The EMBO journal, Vol. 15, Issue 1, pp. 73-82, 1996

Images

Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details Handling References for anti-Phospholipase C gamma 1 Antibody (ABIN967699) back to top
Supplier Images
Western Blotting (WB) image for anti-Phospholipase C gamma 1 antibody (phospholipase C, gamma 1) (AA 82-100) (ABIN967699) Western blot analysis of Phospholipase Cgamma1 on a A431 cell lysate (Human epithelia...
Immunofluorescence (IF) image for anti-Phospholipase C gamma 1 antibody (phospholipase C, gamma 1) (AA 82-100) (ABIN967699) Immunofluorescence staining of MCF7 cells (Human breast adenocarcinoma, ATCC HTB-22).
 image for anti-Phospholipase C gamma 1 antibody (phospholipase C, gamma 1) (AA 82-100) (ABIN967699) anti-phospholipase C, gamma 1 (PLCG1) (AA 82-100) antibody (Image 3)
Western Blotting (WB) image for anti-Phospholipase C gamma 1 antibody (phospholipase C, gamma 1) (AA 82-100) (ABIN967699) anti-phospholipase C, gamma 1 (PLCG1) (AA 82-100) antibody (Image 4)