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anti-Human Phospholipase C gamma 1 antibody for Intracellular Staining

Recommended Phospholipase C gamma 1 Antibody (supplied by: Log in to see )

Antigen
phospholipase C, gamma 1 (PLCG1) Antibodies
  • AI894140
  • Cded
  • nckap3
  • NCKAP3
  • Phosphb
  • PLC'1
  • Plc-1
  • Plc-gamma1
  • PLC-II
  • plc-ii
  • plc1
  • PLC1
  • plc148
  • PLC148
  • PLCbeta1
  • Plcg-1
  • plcgamma1
  • PLCgamma1
  • PPLCA
  • RATPHOSPHB
Epitope
Tyr783, pTyr783
49
41
13
13
9
8
8
8
6
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
216
132
125
11
8
5
4
1
Host
Mouse
192
28
Clonality
Monoclonal
Conjugate
This Phospholipase C gamma 1 antibody is conjugated to Alexa Fluor 647
5
4
4
4
3
3
3
3
3
3
3
3
Application
Intracellular Staining (ICS)
158
57
49
34
31
27
26
6
4
3
2
1
1
1
1
Supplier
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Catalog No. ABIN1177236
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008676636 ABIN1177237 ICS Alexa Fluor 488 Mouse IgG1 Tyr783, pTyr783 Log in to see 27-PLC
0.0008676636 ABIN1176864 ICS Alexa Fluor 647 Mouse IgG1 N-Term Log in to see 10-PLCgamma
0.0008676636 ABIN1176865 ICS Alexa Fluor 488 Mouse IgG1 N-Term Log in to see 10-PLCgamma

Top referenced anti-Phospholipase C gamma 1 antibody for Intracellular Staining

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General

Antigen phospholipase C, gamma 1 (PLCG1) Antibodies
Epitope Tyr783, pTyr783
(49), (41), (13), (13), (9), (8), (8), (8), (6), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(216), (132), (125), (11), (8), (5), (4), (1)
Host Mouse
(192), (28)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Phospholipase C gamma 1 antibody is conjugated to Alexa Fluor 647
(5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3)
Application Intracellular Staining (ICS)
(158), (57), (49), (34), (31), (27), (26), (6), (4), (3), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Phospholipase C gamma 1 Antibody

Target Details Phospholipase C gamma 1 Application Details Handling References for anti-Phospholipase C gamma 1 Antibody (ABIN1177236) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated peptide including the tyrosine 783 of human PLCgamma1
Clone 27-PLC
Isotype IgG1

Target Details Phospholipase C gamma 1

Product Details anti-Phospholipase C gamma 1 Antibody Application Details Handling References for anti-Phospholipase C gamma 1 Antibody (ABIN1177236) Images back to top
Antigen
Alternative Name PLC-gamma 1 (PLCG1 Antibody Abstract)
Background The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLCg is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLCg1, which is ubiquitously expressed, and 2) PLCg2, found primarily in the lymphoid system. PLCg is essential for growth factor-induced cell motility and mitogenesis. PLCg1-null mice exhibit retarded embryonic growth and lethality in midgestation. In addition, PDGF stimulation leads to phosphorylation of PLCg1 at Tyr 783 and activation of hydrolyzing activity. Overexpression of PLCg is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus, regulation of PLCg activity by growth factors is involved in cell growth and transformation. The 27/PLC antibody recognizes PLCg1 phosphorylated at Y783. The fluorochrome-conjugated formats have been evaluated using a human model system. However, the unconjugated form of this antibody (Cat. no. 612464) has been shown to react with human, mouse, and rat cell lysates by western blot.
Pathways RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Handling References for anti-Phospholipase C gamma 1 Antibody (ABIN1177236) Images back to top
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details References for anti-Phospholipase C gamma 1 Antibody (ABIN1177236) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References for anti-Phospholipase C gamma 1 Antibody (ABIN1177236)

Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details Handling Images back to top
Product cited in:

Yu, Fukami, Itoh, Takenawa: "Phosphorylation of phospholipase Cgamma1 on tyrosine residue 783 by platelet-derived growth factor regulates reorganization of the cytoskeleton." in: Experimental cell research, Vol. 243, Issue 1, pp. 113-22, 1998