anti-Human Phospholipase C gamma 1 antibody for Immunofluorescence

Recommended Phospholipase C gamma 1 Antibody (supplied by: Log in to see )

Antigen
phospholipase C, gamma 1 (PLCG1) Antibodies
  • PLC'1
  • PLCbeta1
  • Phosphb
  • RATPHOSPHB
  • plc1
  • nckap3
  • plc-ii
  • plc148
  • plcgamma1
  • NCKAP3
  • PLC-II
  • PLC1
  • PLC148
  • PLCgamma1
  • AI894140
  • Cded
  • Plc-1
  • Plc-gamma1
  • Plcg-1
  • PPLCA
  • phospholipase C, beta 1 (phosphoinositide-specific)
  • phospholipase C, gamma 1
  • Plcb1
  • plcg1
  • PLCG1
  • Plcg1
  • LOC100349958
Epitope
AA 82-100
73
44
15
14
13
9
7
6
5
5
5
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
307
194
186
40
17
10
6
4
4
3
2
1
1
1
Host
Mouse
217
92
Clonality
Monoclonal
Conjugate
This Phospholipase C gamma 1 antibody is un-conjugated
8
8
7
7
6
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
237
96
91
46
43
41
38
26
20
4
4
4
2
2
1
1
1
Options
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Catalog No. ABIN967699
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.176395 ABIN1532393 IF ELISA Rabbit IgG Log in to see Polyclonal 2
11.176395 ABIN562265 IF PLA ELISA WB Mouse IgG1 kappa AA 1192-1291, partial Log in to see 2A2 1
11.176395 ABIN2705140 IC IF WB Rabbit C-Term, pTyr1253 Log in to see Polyclonal
11.176395 ABIN2705141 IC IF WB Rabbit C-Term Log in to see Polyclonal
11.176395 ABIN1870509 IF WB Rabbit IgG pTyr771 Log in to see Polyclonal
11.176395 ABIN4346122 ELISA ICC IF IHC IHC (p) IP WB Mouse IgM Log in to see 2C11
11.176395 ABIN4346124 ICC IF IHC IHC (p) Rabbit Log in to see Polyclonal
11.176395 ABIN4346125 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
11.176395 ABIN3020016 IF WB Rabbit pTyr771 Log in to see Polyclonal
11.176395 ABIN349181 IF Rabbit IgG pTyr771 Log in to see Polyclonal
11.176395 ABIN349182 IF Rabbit IgG pTyr771 Log in to see Polyclonal
1 ABIN401590 IF WB Rabbit pTyr771 Log in to see Polyclonal 3
1 ABIN362456 IF Rabbit IgG pTyr771 Log in to see Polyclonal 2
1 ABIN393550 IF WB Mouse IgG1 kappa AA 1192-1292 Log in to see 2A2
1 ABIN2888691 IF ELISA Rabbit IgG AA 1221-1270 Log in to see Polyclonal
1 ABIN597107 IF Rabbit IgG pTyr771 Log in to see Polyclonal
1 ABIN296076 FACS IF ELISA WB Rabbit IgG pTyr783 Log in to see Polyclonal
1 ABIN3186502 ELISA IF WB Rabbit IgG Thr202, Thr236 Log in to see Polyclonal
1 ABIN4346129 CyTOF ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 3C2D11
1 ABIN4346130 CyTOF ELISA FACS ICC IF IHC IHC (p) WB Mouse Log in to see 3H1C10

General

Antigen phospholipase C, gamma 1 (PLCG1) Antibodies
Epitope AA 82-100
(73), (44), (15), (14), (13), (9), (7), (6), (5), (5), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(307), (194), (186), (40), (17), (10), (6), (4), (4), (3), (2), (1), (1), (1)
Host Mouse
(217), (92)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Phospholipase C gamma 1 antibody is un-conjugated
(8), (8), (7), (7), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(237), (96), (91), (46), (43), (41), (38), (26), (20), (4), (4), (4), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Phospholipase C gamma 1 Antibody

Target Details Phospholipase C gamma 1 Application Details Handling References for anti-Phospholipase C gamma 1 antibody (ABIN967699) Images
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Cow PLCgamma1 aa. 82-100
Clone 10-PLCgamma
Isotype IgG1

Target Details Phospholipase C gamma 1

Product Details anti-Phospholipase C gamma 1 Antibody Application Details Handling References for anti-Phospholipase C gamma 1 antibody (ABIN967699) Images back to top
Antigen
Alternative Name Phospholipase C gamma 1 (PLCG1 Antibody Abstract)
Background The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLCg is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLCg1, which is ubiquitously expressed, and 2) PLCg2, found primarily in the lymphoid system. PLCg is essential for growth factor-induced cell motility and mitogenesis. PLCg1 null mice exhibit retarded embryonic growth and lethality in midgestation. Overexpression of PLCg is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus regulation of PLCg activity by growth factors is involved in cell growth and transformation.
The 10/PLCgamma monoclonal antibody recognizes PLCg1, regardless of phosphorylation status. It does not cross-react with PLCg2.
Molecular Weight 148 kDa
Pathways RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Handling References for anti-Phospholipase C gamma 1 antibody (ABIN967699) Images back to top
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details References for anti-Phospholipase C gamma 1 antibody (ABIN967699) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-Phospholipase C gamma 1 antibody (ABIN967699)

Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details Handling Images back to top
Product cited in:

Vossmeyer, Hofmann, Löster, Reutter, Danker: "Phospholipase Cgamma binds alpha1beta1 integrin and modulates alpha1beta1 integrin-specific adhesion." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4636-43, 2002

Dayanir, Meyer, Lashkari, Rahimi: "Identification of tyrosine residues in vascular endothelial growth factor receptor-2/FLK-1 involved in activation of phosphatidylinositol 3-kinase and cell proliferation." in: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 17686-92, 2001

Harder, Kuhn: "Selective accumulation of raft-associated membrane protein LAT in T cell receptor signaling assemblies." in: The Journal of cell biology, Vol. 151, Issue 2, pp. 199-208, 2000

Chen, Murphy-Ullrich, Wells: "A role for gelsolin in actuating epidermal growth factor receptor-mediated cell motility." in: The Journal of cell biology, Vol. 134, Issue 3, pp. 689-98, 1996

Obermeier, Tinhofer, Grunicke, Ullrich: "Transforming potentials of epidermal growth factor and nerve growth factor receptors inversely correlate with their phospholipase C gamma affinity and signal activation." in: The EMBO journal, Vol. 15, Issue 1, pp. 73-82, 1996

Images

Product Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details Handling References for anti-Phospholipase C gamma 1 antibody (ABIN967699) back to top
Supplier Images
Western Blotting (WB) image for anti-phospholipase C, gamma 1 (PLCG1) (AA 82-100) antibody (ABIN967699) Western blot analysis of Phospholipase Cgamma1 on a A431 cell lysate (Human epithelia...
Immunofluorescence (IF) image for anti-phospholipase C, gamma 1 (PLCG1) (AA 82-100) antibody (ABIN967699) Immunofluorescence staining of MCF7 cells (Human breast adenocarcinoma, ATCC HTB-22).
 image for anti-phospholipase C, gamma 1 (PLCG1) (AA 82-100) antibody (ABIN967699) anti-phospholipase C, gamma 1 (PLCG1) (AA 82-100) antibody (Image 3)
Western Blotting (WB) image for anti-phospholipase C, gamma 1 (PLCG1) (AA 82-100) antibody (ABIN967699) anti-phospholipase C, gamma 1 (PLCG1) (AA 82-100) antibody (Image 4)