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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||202 Antibodies||105 Antibodies||99 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Intracellular Staining (ICS)||4 Antibodies||2 Antibodies||2 Antibodies|
|Immunoprecipitation (IP)||23 Antibodies||5 Antibodies||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||33 Antibodies||20 Antibodies||19 Antibodies|
|Immunohistochemistry (IHC)||67 Antibodies||44 Antibodies||42 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||34 Antibodies||34 Antibodies||34 Antibodies|
|Antigen||phospholipase C, gamma 1 (PLCG1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Phospholipase C gamma 1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Phospholipase C gamma 1 AntibodyTarget Details Phospholipase C gamma 1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of PLC γ1 protein.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % (by SDS-PAGE)|
Target Details Phospholipase C gamma 1Product Details anti-Phospholipase C gamma 1 Antibody Application Details Handling Images back to top
|Alternative Name||PLCG1 (PLCG1 Antibody Abstract)|
|Background||Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC ε. PLC γ1 is widely distributed in bronchiolar epithelium, type I and II pneumocytes and fibroblasts of the interstitial tissue. Actinregulatory protein Villin is tyrosine phosphorylated and associates with PLC γ1 in the brush border of intestinal epithelial cells. Villin regulates PLC γ1 activity by modifying its own ability to bind phosphatidylinositol 4,5-biphosphate. PLC γ1 binds α1β1 Integrin and modulates α1β1 Integrin-specific adhesion. PLC γ1 and Ca2+ play a direct role in VEGF-regulated endothelial growth, however this signaling pathway is not linked to FGF-mediated effects in primary endothelial cells.Synonyms: 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1, PLC gamma1 Phospholipase C-II, PLC-148, PLC-II, PLC-gamma-1, PLC1, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-gamma-1|
|Molecular Weight||approx. 155 kDa|
|Pathways||RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Handling Images back to top
Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-Phospholipase C gamma 1 Antibody Target Details Phospholipase C gamma 1 Application Details Handling back to top