anti-Human Phospholipase D2 antibody for Western Blotting

Recommended Phospholipase D2 Antibody (supplied by: Log in to see )

Antigen
Phospholipase D2 (PLD2) Antibodies
  • PLD2
  • Pldc
  • phospholipase D2
  • PLD2
  • pld2
  • CpipJ_CPIJ013122
  • VDBG_00892
  • Pld2
Epitope
Middle Region
16
15
14
7
3
2
2
2
1
1
1
Reactivity
Cow (Bovine), Horse (Equine), Human, Pig (Porcine), Rat (Rattus)
57
28
17
8
4
2
2
2
2
1
Host
Rabbit
48
9
Clonality
Polyclonal
Conjugate
This Phospholipase D2 antibody is un-conjugated
2
2
2
2
2
2
Application
Western Blotting (WB)
52
30
24
11
4
4
1
1
Options
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Catalog No. ABIN2777719
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.906538 ABIN2780445 WB Rabbit N-Term Log in to see Polyclonal 1
2.906538 ABIN954193 EIA IHC (p) WB Rabbit Ig AA 9-39, N-Term Log in to see Polyclonal 4
2.906538 ABIN1532165 IHC ELISA WB Rabbit IgG pTyr169 Log in to see Polyclonal
2.906538 ABIN2706804 WB Rabbit pTyr169 Log in to see Polyclonal
2.906538 ABIN562269 ELISA WB Mouse IgG2a kappa AA 834-933, partial Log in to see 1C5 1
2.906538 ABIN2457997 ELISA WB Rabbit Log in to see Polyclonal
2.906538 ABIN2458301 ELISA WB Rabbit Log in to see Polyclonal
2.906538 ABIN658675 WB Rabbit Ig AA 10-39, N-Term Log in to see Polyclonal
2.906538 ABIN4904788 WB Rabbit IgG Log in to see Polyclonal
2.906538 ABIN927421 WB Rabbit Middle Region Log in to see Polyclonal
2.906538 ABIN927701 WB Rabbit N-Term Log in to see Polyclonal
2.906538 ABIN5542411 ELISA FACS ICC IHC WB Mouse IgG1 AA 834-933 Log in to see 7E4D9
2.906538 ABIN5542412 ELISA FACS ICC IHC WB Mouse IgG1 AA 834-933 Log in to see 8G1G2
2.906538 ABIN1847810 IHC ELISA WB Rabbit Internal Region, pTyr169 Log in to see Polyclonal
1 ABIN393480 ELISA WB Mouse IgG2a kappa AA 834-934 Log in to see 1C5 1
1 ABIN463031 WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN462790 WB Rabbit IgG AA 467-516 Log in to see Polyclonal
1 ABIN2568438 IHC WB Rabbit IgG pTyr169 Log in to see Polyclonal
1 ABIN4880952 ELISA FACS ICC IHC WB Mouse IgG1 AA 834-933 Log in to see 7E4D9
1 ABIN4880953 ELISA FACS WB Mouse IgG1 AA 834-933 Log in to see 8G1G2

General

Antigen Phospholipase D2 (PLD2) Antibodies
Epitope Middle Region
(16), (15), (14), (7), (3), (2), (2), (2), (1), (1), (1)
Reactivity Cow (Bovine), Horse (Equine), Human, Pig (Porcine), Rat (Rattus)
(57), (28), (17), (8), (4), (2), (2), (2), (2), (1)
Host Rabbit
(48), (9)
Clonality
Polyclonal
Conjugate This Phospholipase D2 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(52), (30), (24), (11), (4), (4), (1), (1)
Pubmed 1 reference available
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Product Details anti-Phospholipase D2 Antibody

Target Details Phospholipase D2 Application Details Handling References for anti-Phospholipase D2 antibody (ABIN2777719) Images
Blocking Peptide Blocking peptide for this product available: ABIN981844
Sequence DLHYRLTDLG DSSESAASQP PTPRPDSPAT PDLSHNQFFW LGKDYSNLIT
Predicted Reactivity Cow: 100%, Horse: 92%, Human: 100%, Pig: 92%, Rat: 92%
Characteristics This is a rabbit polyclonal antibody against PLD2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human PLD2

Target Details Phospholipase D2

Product Details anti-Phospholipase D2 Antibody Application Details Handling References for anti-Phospholipase D2 antibody (ABIN2777719) Images back to top
Antigen
Alternative Name PLD2 (PLD2 Antibody Abstract)
Background Phosphatidylcholine (PC)-specific phospholipases D (PLDs, EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.Phosphatidylcholine (PC)-specific phospholipases D (PLDs, EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-14 AF033850.1 61-74 15-614 BC015033.1 1-600 615-1279 BC056871.1 549-1213 1280-1830 BC015033.1 1266-1816 1831-1995 BC056871.1 1765-1929 1996-3489 BC015033.1 1982-3475
Alias Symbols: -
Protein Interaction Partner: UBC, RPS6KB1, CUL3, AQP3, RPTOR, PEA15, PIP5K1A, STXBP1, DPYSL2, BIN1, MTOR, CAV1, AMPH, OPRM1, ALDOC, GM2A, PRKCD, PRKCA, PLCG1, LCK, GAPDH, FYN, EGFR, CSK, ALDOA, ARF1, ACTB, ACTN1, CAV2, SRC,
Protein Size: 933
Molecular Weight 106 kDa
Gene ID 5338
NCBI Accession NM_002663, NP_002654
UniProt O14939
Pathways RTK Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-Phospholipase D2 Antibody Target Details Phospholipase D2 Handling References for anti-Phospholipase D2 antibody (ABIN2777719) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 933 AA

Restrictions For Research Use only

Handling

Product Details anti-Phospholipase D2 Antibody Target Details Phospholipase D2 Application Details References for anti-Phospholipase D2 antibody (ABIN2777719) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Phospholipase D2 antibody (ABIN2777719)

Product Details anti-Phospholipase D2 Antibody Target Details Phospholipase D2 Application Details Handling Images back to top
Background publications

Gorshkova, He, Berdyshev, Usatuyk, Burns, Kalari, Zhao, Pendyala, Garcia, Pyne, Brindley, Natarajan: "Protein kinase C-epsilon regulates sphingosine 1-phosphate-mediated migration of human lung endothelial cells through activation of phospholipase D2, protein kinase C-zeta, and Rac1." in: The Journal of biological chemistry, Vol. 283, Issue 17, pp. 11794-806, 2008

Images

Product Details anti-Phospholipase D2 Antibody Target Details Phospholipase D2 Application Details Handling References for anti-Phospholipase D2 antibody (ABIN2777719) back to top
Supplier Images
Western Blotting (WB) image for anti-Phospholipase D2 (PLD2) (Middle Region) antibody (ABIN2777719) anti-Phospholipase D2 (PLD2) (Middle Region) antibody
Western Blotting (WB) image for anti-Phospholipase D2 (PLD2) (Middle Region) antibody (ABIN2777719) anti-Phospholipase D2 (PLD2) (Middle Region) antibody (Image 2)