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anti-Rat (Rattus) Phospholipase D2 antibody for Western Blotting

Recommended Phospholipase D2 Antibody (supplied by: Log in to see )

Antigen
Phospholipase D2 (PLD2) Antibodies
  • PLD2
  • Pldc
Epitope
Middle Region
23
17
12
2
2
2
2
2
2
1
1
Reactivity
Cow (Bovine), Human, Rat (Rattus)
52
17
10
5
2
1
Host
Rabbit
47
5
Clonality
Polyclonal
Conjugate
This Phospholipase D2 antibody is un-conjugated
2
2
2
2
2
2
Application
Western Blotting (WB)
45
28
22
7
1
1
1
Supplier
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Catalog No. ABIN2777719
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN1532165 IHC ELISA WB Rabbit IgG pTyr169 Log in to see Polyclonal
0.13341247 ABIN2706804 WB Rabbit pTyr169 Log in to see Polyclonal
0.13341247 ABIN2498981 IHC ELISA WB Rabbit IgG pTyr169 Log in to see Polyclonal
0.13341247 ABIN927421 WB Rabbit Middle Region Log in to see Polyclonal
0.13341247 ABIN1847810 IHC ELISA WB Rabbit Internal Region, pTyr169 Log in to see Polyclonal
0.0011728023 ABIN2568438 IHC WB Rabbit IgG pTyr169 Log in to see Polyclonal
0.0011728023 ABIN2498980 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN2149253 IHC ELISA WB Rabbit IgG pTyr169 Log in to see Polyclonal
0.0011728023 ABIN1578922 IHC ELISA WB Rabbit pTyr169 Log in to see Polyclonal

Top referenced anti-Phospholipase D2 antibody for Western Blotting

  • Cow (Bovine) Polyclonal Phospholipase D2 Primary Antibody for WB - ABIN2777719 (1 Publications): Gorshkova, He, Berdyshev, Usatuyk, Burns, Kalari, Zhao, Pendyala, Garcia, Pyne, Brindley, Natarajan: Protein kinase C-epsilon regulates sphingosine 1-phosphate-mediated migration of human lung endothelial cells through activation of phospholipase D2, protein kinase C-zeta, and Rac1. in The Journal of biological chemistry 2008 (PubMed)

Similar anti-Phospholipase D2 Antibodies

Application / Reactivity Human Cow (Bovine) Rat (Rattus)
Western Blotting (WB) 46 Antibodies 5 Antibodies 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunohistochemistry (IHC) 22 Antibodies 6 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
ELISA 28 Antibodies 6 Antibodies

General

Antigen Phospholipase D2 (PLD2) Antibodies
Epitope Middle Region
(23), (17), (12), (2), (2), (2), (2), (2), (2), (1), (1)
Reactivity Cow (Bovine), Human, Rat (Rattus)
(52), (17), (10), (5), (2), (1)
Host Rabbit
(47), (5)
Clonality
Polyclonal
Conjugate This Phospholipase D2 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(45), (28), (22), (7), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Phospholipase D2 Antibody

Target Details Phospholipase D2 Application Details Handling References for anti-Phospholipase D2 Antibody (ABIN2777719) Images
Blocking Peptide Blocking peptide for this product available: ABIN981844
Sequence DLHYRLTDLG DSSESAASQP PTPRPDSPAT PDLSHNQFFW LGKDYSNLIT
Predicted Reactivity Bovine : 100 %, Human : 100 %, Pig : 90 %, Rat : 80 %
Characteristics This is a rabbit polyclonal antibody against PLD2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human PLD2

Target Details Phospholipase D2

Product Details anti-Phospholipase D2 Antibody Application Details Handling References for anti-Phospholipase D2 Antibody (ABIN2777719) Images back to top
Antigen
Alternative Name PLD2 (PLD2 Antibody Abstract)
Background Phosphatidylcholine (PC)-specific phospholipases D (PLDs, EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.Phosphatidylcholine (PC)-specific phospholipases D (PLDs, EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.
Molecular Weight 106 kDa
Gene ID 5338
NCBI Accession NP_002654, NM_002663
UniProt O14939
Pathways RTK Signaling

Application Details

Product Details anti-Phospholipase D2 Antibody Target Details Phospholipase D2 Handling References for anti-Phospholipase D2 Antibody (ABIN2777719) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 933 AA

Restrictions For Research Use only

Handling

Product Details anti-Phospholipase D2 Antibody Target Details Phospholipase D2 Application Details References for anti-Phospholipase D2 Antibody (ABIN2777719) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Phospholipase D2 Antibody (ABIN2777719)

Product Details anti-Phospholipase D2 Antibody Target Details Phospholipase D2 Application Details Handling Images back to top
Background publications

Gorshkova, He, Berdyshev, Usatuyk, Burns, Kalari, Zhao, Pendyala, Garcia, Pyne, Brindley, Natarajan: "Protein kinase C-epsilon regulates sphingosine 1-phosphate-mediated migration of human lung endothelial cells through activation of phospholipase D2, protein kinase C-zeta, and Rac1." in: The Journal of biological chemistry, Vol. 283, Issue 17, pp. 11794-806, 2008

Images

Product Details anti-Phospholipase D2 Antibody Target Details Phospholipase D2 Application Details Handling References for anti-Phospholipase D2 Antibody (ABIN2777719) back to top
Supplier Images
Western Blotting (WB) image for anti-Phospholipase D2 antibody (PLD2) (Middle Region) (ABIN2777719) anti-Phospholipase D2 (PLD2) (Middle Region) antibody
Western Blotting (WB) image for anti-Phospholipase D2 antibody (PLD2) (Middle Region) (ABIN2777719) anti-Phospholipase D2 (PLD2) (Middle Region) antibody (Image 2)